Synchronic measurements of spontaneous ultra-weak light emission from germinating wheat seedlings both in Brazil and after transportation to Japan, and with a simultaneous series of germinations with local seedlings in the Czech Republic, are presented. A series of tests was also performed with samples returned from Japan to Brazil and results compared with those from undisturbed Brazilian seedlings. Native seedlings presented semi-circadian rhythms of emission which correlated with the gravimetric tidal acceleration at their locality, as did seeds which had been transported from Brazil to Japan, and then returned to Brazil. Here, however, there were very small disturbances within the periodicity of emissions, perhaps as a result of similar tidal profiles at locations whose longitudes are 180° apart, as in this case, different from previous results obtained in Brazil-Germany tests with other longitude shift. This feature of the Brazil and Japan locations may have minimized the requirement for the acclimatization of the transported seed to their new location.

• MeSH
• cestování letadlem MeSH
• cirkadiánní rytmus * MeSH
• fotony * MeSH
• gravitace * MeSH
• klíčení * MeSH
• pšenice fyziologie   MeSH
• semenáček fyziologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

The surface plasmon resonance (SPR) biosensor system with dispersionless microfluidics for the direct and label-free detection of a soluble vascular endothelial growth factor receptor (sVEGFR-1) is described. The detection approach takes advantage of an affinity interaction between sVEGFR-1 and its ligand, vascular endothelial growth factor (VEGF-A), which is covalently immobilized on the surface of the SPR sensor. The ability of the immobilized VEGF-A to specifically bind the sVEGFR-1 receptor is demonstrated in a buffer. The detection of sVEGFR-1 in 2% human blood plasma is carried out by using the sequential injection approach. The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use.

• MeSH
• biologické markery krev   MeSH
• biosenzitivní techniky metody   MeSH
• lidé MeSH
• myelodysplastické syndromy krev   diagnóza   metabolismus   MeSH
• povrchová plasmonová rezonance metody   MeSH
• receptor 1 pro vaskulární endoteliální růstový faktor krev   metabolismus   MeSH
• vaskulární endoteliální růstový faktor A krev   metabolismus   MeSH
• vazba proteinů MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH