In the interests of satisfying the dental services demands of German citizens area-wide, constant, and thoughtful planning of supply and demand is essential. With an anonymous online survey of 375 dentists a pairwise comparison of 9 factors extracted as relevant from the existing scientific literature were analyzed with an analytic hierarchy process (AHP) and ranked considering the various business types. In general, 5 local environmental factors have a dominant impact on founders' decision in German dentistry. In order: environment for the family, quality of life in private environment, real income, location of the practice, infrastructure. Real income is in first place (p = 0.287) for dentists who want to start a new single practice. For preferring a new community practice, it is on third place (p = 0.177) and for dentists who favor a takeover a single practice (p = 0.130) or joining a community practice (p = 0.096) or employment (p = 0.111) it is fourth place. For this purpose, the location of the practice is of greater priority than the real income for dentists who prefer not to start a new practice. The AHP method is a way to picture a priority list out of all relevant factors for setting up of a dental practice.
- MeSH
- analytický hierarchický proces * MeSH
- kvalita života MeSH
- lidé MeSH
- průzkumy a dotazníky MeSH
- zaměstnanost MeSH
- zubní lékaři * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Lactobacillus, Bifidobacterium and Enterococcus cultures are increasingly used as probiotics for humans and pigs. The aim of this study was to investigate if co-cultivation of porcine and human neutrophils with probiotics can lead to increased apoptosis in vitro. Ten adult Large white pigs and 10 healthy human donors were used in this study. Neutrophils were isolated by dextran sedimentation and cultivated with and without the lactic acid bacteria Bifidobacterium bifidum, Lactobacillus rhamnosus, and Enterococcus faecium for 2, 4, 24 and 48 h. Early and late apoptosis was measured using flow cytometry, and cell lysis was detected based on lactate dehydrogenase activity (LDH). A significant (P < 0.05; P < 0.01) increase in apoptotic neutrophils and LDH was observed at 24 h and 48 h in vitro. All probiotics exerted their greatest effects on the early apoptosis of porcine neutrophils, while the effects of L. rhamnosus were most pronounced on late apoptosis and those of B. bifidumon LDH release of human neutrophils. The increased neutrophil apoptosis caused by probiotic bacteria can be beneficial for more efficient efferocytosis and faster resolution of inflammation and tissue regeneration. In conclusion, we have demonstrated that the interaction of B. bifidum, L. rhamnosus,and E. faecium with human and porcine neutrophils leads to their apoptosis.
- MeSH
- apoptóza MeSH
- Bifidobacterium bifidum fyziologie imunologie MeSH
- Enterococcus faecium fyziologie imunologie MeSH
- Lacticaseibacillus rhamnosus fyziologie imunologie MeSH
- lidé MeSH
- neutrofily MeSH
- probiotika * aplikace a dávkování farmakologie metabolismus MeSH
- průtoková cytometrie metody MeSH
- Sus scrofa MeSH
- techniky in vitro MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
