Human's fetal hepatocytes (HFH) were intrasplenic transplanted white non-pedigree rats with acute liver failure (ALF) challenged by single per oral administration of hepatotropic toxin diluted in oil ССl4 at a dose 10 ml/kg (volumetric correlation 1:1) (10 mL/kg body weight as a 1:1 mixture of CCl4 and mineral oil). Transplantation had positive effect on all biochemical blood parameters of the studying animals. Morphologic study showed that reparative-restorative processes were arising in hepatic parenchyma after administration of HFH into splenic pulp of rats with model of ALF on days 14-21. Substantial and main factor in restoration of parenchyma was restoration of micro topographic interrelations in acinus as well as polyploidy of hepatic cells expressed in increase of hepatocytes' nuclei sizes and hypertrophy of cells themselves. It is an indirect confirmation of engraftment of HFH in liver of rats with model of ALF.
Treatment results of postoperative peritonitis (PP) in 89 patients have been analyzed. All repeated laparoscopy interventions in early postoperative period both after laparoscopic and conventional laparotomy interventions are combined under term "re-laparoscopy". The patients were divided into two groups depending on a kind of repeated operative intervention. The 1st group formed 62 (69.7%) patients with PP sustained re-laparotomy in postoperative period. The 2nd main group joined 27 (30.3%) patients experienced beginning interventions with video endoscopy study. Repeated laparoscopy intervention in PP allowed diagnosing a postoperative intraabdominal complication in 85.4% patients, 55.2% of them avoided laparotomy due to application of laparoscopy methods, 30% of them avoided re-laparotomy. Indices of lethality in patients underwent laparoscopy (3 of 27 patients died) were reliably lower compared (χ2= 8.62, df=1, р=0.003) to patients in control group (43 of 62 patients died); the result was determined with an earlier low invasive intervention as well as by lower level of its surgical aggression.
- MeSH
- chirurgie operační MeSH
- dospělí MeSH
- laparoskopie * využití MeSH
- lidé středního věku MeSH
- lidé MeSH
- peritonitida * chirurgie MeSH
- pooperační období MeSH
- reoperace MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- výsledky a postupy - zhodnocení (zdravotní péče) MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
Small and large intestines' injuries in the structure of the abdominal traumas make 6-25% according to various studies. The surgeons have a difficult task of carrying the medical aid for this type of patients because there is still no the common classification and treating tactics for the cure of small and large intestines' injuries. We observed cases of 167 patients with small and large intestines' injuries treated in RRCEM (Republican Research Centre of Emergency Medicine, Uzbekistan, Tashkent) from 2001 till 2009. According to the type of the traumas the patients were classified as following: penetrating cut-stab wounds - 115 (68.6%), closed abdominal injury - 50 (30%) and gunshot wounds in 2 (1.4%) cases. 70% of the patients with this pathology were admitted in the shock condition. The different complications were noted in 50 (30%) patients after the operation. The hardest group of complications is intestine sutures' failure: in 4 cases it was intestine sutures' failure and 3 patients had the failure of anastomosis sutures. Large and small intestine injuries' complications led to lethal result for 4(2%) patients.
99 patients with diagnosis of diffuse peritonitis (DP) were examined. There were 64 (64.6%) males and 35 (35.4%) females, an average age was 44.07±19.15 (±SD), range - 15-83 years. Systemic inflammatory reaction manifesting in clinic of DP and abdominal sepsis were provoked first of all by Klebsiella spp. (20, 20.2%) and Ваcteroides fragilis (24, 24.2%) possessing the greatest virulence compared with other intestinal bacteria. Exudates of the died patients differed from the survived ones by prevalence of gram-negative microflora (60.6%) in association with gram-positive microflora (24.2%) and anaerobes (48.5%). Though specific weight of these microorganisms was higher by 3.07, 1.45 and 2.46 times from survived patients.
- MeSH
- dospělí MeSH
- exsudáty a transsudáty * mikrobiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mikrobiologické techniky metody MeSH
- mladiství MeSH
- mladý dospělý MeSH
- peritonitida * mikrobiologie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- sepse mikrobiologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
Two-staged investigations were done in patients at the age from 19 to 85 who were treated in Republican Research Centre of Emergency Medicine from 2003 to 2009. At the first stage for the creating the estimation scale for severity of condition and prognosis of peritonitis (ESSCPP) it was done the retrospective analysis of 422 history cases of the patients with GP (156 died and 266 survived patients). The modeling of heaviness condition of patients with diagnosis GP was made with the use of the logistic regressive model by condition “survived/died”. At the second stage the prospective investigation of ESSCPP in 72 patients with GP had been carried. The estimation of clinic-laboratory parameters, functional status of bowels and prevalence of the purulent process in the abdominal cavity at the first step had been retrospectively done in 422 patients according to 13 independent variables. The result of the prospective comparative data of ROC-analysis of ESSCPP with APACHE II and MPI scales showed that it had the high diagnostic significance in the biggest area under ROC-curve (AUC=0.946±0.013).
- MeSH
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- matematika * MeSH
- mladý dospělý MeSH
- peritonitida * patofyziologie MeSH
- prognóza * MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- stupeň závažnosti nemoci MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- senioři nad 80 let MeSH
- senioři MeSH
