In the light of recent advances in sequencing, which is becoming increasingly available, the microarray technology has partly assumed a complementary and application role. The last two decades, however, brought into application a genuine microarray approaches and technologies, especially population (VIPTM) chips, enabling one to costeffectively screen populations of tens of thousands of individuals (from humans to microorganisms) and thus effectively generate solutions for personalized medicine as well as multi-patient studies and therapies of neurodegenerative diseases. The paper summarizes basic and advanced contemporary microarray platforms and biomarkers useful for the research and diagnostics of neurodegenerative diseases including the microarray state of the art population (VIPTM) chips. Since the development and function of the nervous system is highly dependent on the products of gene expression, individual genotype and phenotype, the described diagnostic tools will likely contribute to a highly demanded elucidation of the molecular mechanisms and therapeutic solutions for neurodegenerative diseases.
- Klíčová slova
- Ames test,
- MeSH
- celulosa oxidovaná MeSH
- farmaceutické pomocné látky MeSH
- léčivé přípravky MeSH
- lidé MeSH
- nutrigenomika MeSH
- poškození DNA MeSH
- rychlé screeningové testy MeSH
- testy genotoxicity MeSH
- vysoce účinné nukleotidové sekvenování MeSH
- zdravotnické prostředky MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
In the light of recent advances in sequencing, which is becoming increasingly available, the microarray technology has partly assumed a complementary and application role. The last two decades, however, brought into application a genuine microarray approaches and technologies, especially population (VIPTM) chips, enabling one to costeffectively screen populations of tens of thousands of individuals (from humans to microorganisms) and thus effectively generate solutions for personalized medicine as well as multi-patient studies and therapies of neurodegenerative diseases. The paper summarizes basic and advanced contemporary microarray platforms and biomarkers useful for the research and diagnostics of neurodegenerative diseases including the microarray state of the art population (VIPTM) chips. Since the development and function of the nervous system is highly dependent on the products of gene expression, individual genotype and phenotype, the described diagnostic tools will likely contribute to a highly demanded elucidation of the molecular mechanisms and therapeutic solutions for neurodegenerative diseases.
- Klíčová slova
- biočipová analýza,
- MeSH
- Alzheimerova nemoc diagnóza MeSH
- biologické markery MeSH
- DNA sondy MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé MeSH
- mikročipová analýza MeSH
- molekulární biologie MeSH
- nádory mozku diagnóza MeSH
- nemoci nervového systému diagnóza MeSH
- neurodegenerativní nemoci diagnóza MeSH
- Parkinsonova nemoc diagnóza MeSH
- plošný screening MeSH
- polymerázová řetězová reakce MeSH
- schizofrenie diagnóza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Composite membranes containing molecular sieve particles embedded in a polyimide matrix are promising due to their increased permeability and high selectivity in gas separation processes. Determination of permeability of dense membranes is time-consuming and the resulting values are loaded with experimental errors. The impact of uncertainty in various quantities on the reliability of the permeability values measured by the constant volume/ variable pressure method was analyzed. The total uncertainty of the measurements on polyimide/Silicalite-1 membranes is 7–13 %, the errors in membrane thickness and permeation coefficient being the main contributing factors.
