Kazuistika pojednává o pacientce s dlouhodobě nedostatečně kompenzovaným diabetem 2. typu, u které bylo dosaženo úspěchu v léčbě nasazením nové fixní kombinace bazálního inzulínu a GLP-1 agonisty.
A case describes a patient with a prolonged inadequate compensation of type 2 diabetes, in whom has success new therapy with fixed combination of insulin degludec and liraglutide.
- MeSH
- diabetes mellitus 2. typu * farmakoterapie MeSH
- dlouhodobě působící inzulin aplikace a dávkování metabolismus MeSH
- fixní kombinace léků MeSH
- glykovaný hemoglobin účinky léků MeSH
- hmotnostní úbytek účinky léků MeSH
- lidé středního věku MeSH
- lidé MeSH
- liraglutid aplikace a dávkování metabolismus MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- MeSH
- aneurysma břišní aorty komplikace diagnostické zobrazování MeSH
- aorta abdominalis diagnostické zobrazování MeSH
- bolesti břicha etiologie MeSH
- cévní píštěle diagnostické zobrazování etiologie MeSH
- lidé MeSH
- multidetektorová počítačová tomografie MeSH
- ruptura aorty komplikace diagnostické zobrazování MeSH
- senioři MeSH
- šok etiologie MeSH
- vena cava inferior diagnostické zobrazování MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
Metallomics is an area that studies the interactions of metal ions with proteins and other biomolecules and their function in living organisms. Metallothioneins (MTs) belong to a large family of metalloproteins in this field. As it is known, the presence of Cd2+ ions causes higher expression of MTs. Therefore we focused on determination of MT and Cd content in liver from chicken embryos (model organism) by electrochemistry and atomic absorption spectroscopy (AAS) after the exposure of chicken embryos to different concentrations of Cd(NO3)2. We also determined the spatial distribution of MT in chicken liver tissue slices by matrix assisted laser desorption/ionization time-of-flight mass spectrometry imaging (MALDI-TOF MSI) and evaluated the toxicity of Cd(NO3)2 to chicken embryos. Finally, we attempted to find the correlation between 2D MSI maps of MT and concentration of MT in chicken liver.
- MeSH
- elektrochemické techniky MeSH
- játra * chirurgie metabolismus účinky léků MeSH
- kadmium * metabolismus toxicita MeSH
- kuřecí embryo MeSH
- metalothionein * chemie metabolismus MeSH
- organokovové sloučeniny toxicita MeSH
- spektrofotometrie atomová MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- zvířata MeSH
- Check Tag
- kuřecí embryo MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
Cancer is a serious disease that causes 25% of deaths in the developed countries. Significant impact on the cancer patients survival has early detection of this disease, therefore great attention is paid to its imaging. Fluorescence imaging represents powerful imaging method for the cell detection. For the successful detection of tumour cells, the specific targeting of fluorescence probes to the tumour tissue has a key role. Interesting materials enabling the imaging of tumour cells are fluorescence nanoparticles. For the accurate imaging, the NPs should be conjugated with targeting ligands and/or constructed as off-on probes.
Doxorubicin (DOX) is an effective antitumor drug employed for treatment of a wide range of cancers types such as neuroblastoma, osteosarcoma, breast and esophageal carcinomas. On the other hand, the cumulative dose is restricted (300-550 mg/m(2)) and its amount administered to a patient has to be closely controlled due to its cardiotoxicity. To understand the mechanisms of the DOX side effects as well as to reveal the ways how to reduce its adverse impact on cardiomyocytes, the interactions with particular components of the blood and tissues have to be studied in greater detail. In this work, microdialysis technique was optimized to extract DOX from samples and subsequently monitor its interaction with BSA. Finally, the microdialysis probe was connected on-line to the LIF detector to ensure the real-time detection. The best flow rate was 1 μL/min and after 120 min of microdialysis 28% of the DOX was dialyzed out from the sample. The results from investigation of the DOX-BSA interaction indicate that the interaction occurs in less than 30 min, causing marked decrease in the amount of DOX extracted by microdialysis.
- MeSH
- doxorubicin chemie MeSH
- fluorescence MeSH
- lidé MeSH
- limita detekce MeSH
- mikrodialýza MeSH
- sérový albumin hovězí chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
There is an arising and concerning issue in the field of bacterial resistance, which is confirmed by the number of deaths associated with drug-resistant bacterial infections. The aim of this study was to compare the effects of antibiotics on Staphylococcus aureus non-resistant strain and strains resistant to cadmium or lead ions. Metal resistant strains were created by the gradual addition of 2 mM solution of metal ions (cadmium or lead) to the S. aureus culture. An increasing antimicrobial effect of ampicillin, streptomycin, penicillin and tetracycline (0, 10, 25, 50, 75, 150, 225 and 300 µM) on the resistant strains was observed using a method of growth curves. A significant growth inhibition (compared to control) of cadmium resistant cells was observed in the presence of all the four different antibiotics. On the other hand, the addition of streptomycin and ampicillin did not inhibit the growth of lead resistant strain. Other antibiotics were still toxic to the bacterial cells. Significant differences in the morphology of cell walls were indicated by changes in the cell shape. Our data show that the presence of metal ions in the urban environment may contribute to the development of bacterial strain resistance to other substances including antibiotics, which would have an impact on public health.
Quantum dots (QDs) are small semiconductor nanoparticles with great optical properties. Their behaviour enables the usage of QDs in in vitro and in vivo experiments and they are promising tools in disease treatment and targeted therapy. The limitation of their usage is the toxicity. Quantum dots consist of different metals, which have various effects on the health. To decrease their toxicity, different surface coatings are used. The effect of QDs on the organism can be tested on chicken embryos. Chicken embryos represent great model for QDs toxicity studies, because there is no need of any permission for the work with embryos and the experiments are low cost and fast.
- MeSH
- dobročinnost MeSH
- finanční podpora výzkumu jako téma MeSH
- nádory MeSH
- Publikační typ
- zprávy MeSH
