Psoriasis is a chronic non-contagious autoimmune disease. Gallic acid is a natural compound with potential health benefits, including antioxidant, anticancer, antiviral and antibacterial properties. Nevertheless, the influence of gallic acid on psoriasis has not been fully determined. This investigation aimed to discover the effect of gallic acid on psoriasis. Thirty-one pairs of psoriatic skin tissues and healthy adult human skin tissues were collected. Human keratinocytes (HaCaT cells) were transfected with interleukin 17A (IL-17A) to create the psoriatic keratinocyte model. The content of bromodomain-containing protein 4 (BRD4) microRNA was assessed using qRT-PCR testing. The content of BRD4 was detected by Western blotting. Cell migration was evaluated by conducting a wound healing assay. Cell proliferation was determined using an EdU assay. Apoptosis was detected by the TUNEL assay. The contents of interferon gamma (IFN-γ), IL-6, IL-8 and IL-17 were detected by ELISA. BRD4 was up-regulated in psoriatic skin tissues and in the IL-17A group compared to the healthy adult human skin tissues and the control group. Silencing BRD4 inhibited cell migration, proliferation and inflammatory response but induced apoptosis in IL-17A-treated HaCaT cells. Conversely, BRD4 over-expression promoted cell migration, proliferation and inflammatory response but suppressed apoptosis in IL-17A-treated HaCaT cells. Gallic acid repressed cell migration, proliferation and inflammatory response but indu-ced apoptosis in HaCaT cells transfected with IL-17A by down-regulating BRD4. Gallic acid represses cell migration, proliferation and inflammatory response but induces apoptosis in IL-17A-transfected HaCaT cells by down-regulating BRD4.
- MeSH
- apoptóza * účinky léků MeSH
- buněčné linie keratinocytů HaCaT MeSH
- buněčné linie MeSH
- dospělí MeSH
- interleukin-17 metabolismus MeSH
- jaderné proteiny metabolismus genetika MeSH
- keratinocyty * účinky léků metabolismus MeSH
- kyselina gallová * farmakologie MeSH
- lidé MeSH
- mikro RNA genetika metabolismus MeSH
- pohyb buněk * účinky léků MeSH
- proliferace buněk * účinky léků MeSH
- proteiny buněčného cyklu * metabolismus genetika MeSH
- proteiny obsahující bromodoménu MeSH
- psoriáza * metabolismus patologie farmakoterapie MeSH
- regulace genové exprese účinky léků MeSH
- transkripční faktory * metabolismus MeSH
- zánět * patologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
