The aim of our study was to develop a model producing obese mice in early adulthood (4-6 weeks) based on their over-nutrition during fetal and early postnatal development. The fertilized dams of the parental generation were fed the standard diet supplemented with high-energy nutritional product Ensure Plus during gestation and lactation. Delivered weanlings were then fed with standard or supplemented diet and assessed for body fat deposits using EchoMRI at the time of early and late adulthood. Maternal over-feeding during the period before weaning had the most significant effect on obesity development in the filial generation. In weanlings, significantly higher body fat deposits and average body weight were recorded. Later, further significant increase in percentage of body fat in both male and female mice was observed. Withdrawal of the Ensure Plus supplement caused a decrease in the percentage of body fat in part of the filial generation. In offspring fed the standard diet, higher fat deposits persisted till the time of late adulthood. We conclude that this diet-induced obesity model might be used in exploration of the effects of elevated body fat on physiological functions of various organ systems during juvenile and early adulthood periods of life of a human being.
- MeSH
- koně MeSH
- lidé MeSH
- modely nemocí na zvířatech * MeSH
- myši inbrední ICR MeSH
- myši MeSH
- nadměrná výživa komplikace metabolismus MeSH
- náhodné rozdělení MeSH
- obezita chemicky indukované etiologie metabolismus MeSH
- skupinový efekt MeSH
- těhotenství MeSH
- tuková tkáň metabolismus MeSH
- věkové faktory MeSH
- vitamin K aplikace a dávkování škodlivé účinky MeSH
- zpožděný efekt prenatální expozice chemicky indukované etiologie metabolismus MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Components of plant essential oils have been reported to have health benefit properties, including antioxidative, anti-tumour, antimicrobial, anti-stress, and immunomodulative activities. We examined the anti-inflammatory effects of thymoquinone, the active ingredient in the volatile oil of Nigella sativa seeds, and borneol, the active component of Salvia officinalis essential oil, on TNBS-induced colitis in mice. Thymoquinone was added to the commercial diet at a concentration of 0.05 % and borneol at two concentrations (0.09% and 0.18%) and fed to ICR mice 5 days before induction of TNBS colitis. Seven days after TNBS administration the mice were killed and macroscopic and histological scores were evaluated. Cytokine mRNA expression in colonic tissue was assessed using quantitative realtime RT-PCR. We did not detect any significant changes in macroscopic and histological scores between experimental and control groups, but we observed a significant decrease in proinflammatory cytokine (IL-1beta and IL-6) mRNA expression in colon tissue in the 0.09% and 0.18% borneol-treated groups of mice in comparison to the control group. Surprisingly, we were not able to confirm anti-inflammatory effects of thymoquinone in TNBS colitis. In conclusion, our data show that borneol is able to significantly suppress proinflammatory cytokine mRNA expression in colonic inflammation, although no significant morphological changes are visible.
- MeSH
- benzochinony farmakologie MeSH
- bornany farmakologie MeSH
- cytokiny genetika metabolismus MeSH
- DNA metabolismus MeSH
- kolitida chemicky indukované patologie MeSH
- kolon patologie účinky léků MeSH
- kyselina trinitrobenzensulfonová farmakologie MeSH
- messenger RNA genetika metabolismus MeSH
- myši MeSH
- polymerázová řetězová reakce s reverzní transkripcí MeSH
- regulace genové exprese účinky záření MeSH
- restrikční enzymy metabolismus MeSH
- tělesná hmotnost účinky léků MeSH
- velikost orgánu účinky léků MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- MeSH
- apoptóza účinky záření MeSH
- blastocysta cytologie MeSH
- daktinomycin farmakologie MeSH
- finanční podpora výzkumu jako téma MeSH
- insulinu podobný růstový faktor I patofyziologie ultrastruktura MeSH
- myši MeSH
- poškození DNA farmakoterapie účinky léků MeSH
- těhotenství MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- těhotenství MeSH
- zvířata MeSH
- MeSH
- bakteriociny farmakologie MeSH
- Enterococcus faecium metabolismus MeSH
- Escherichia coli růst a vývoj účinky léků MeSH
- Listeria růst a vývoj účinky léků MeSH
- mléčné výrobky mikrobiologie MeSH
- ovce MeSH
- Staphylococcus aureus růst a vývoj účinky léků MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Geografické názvy
- Slovenská republika MeSH
- Klíčová slova
- ENTEROCIN CCM 4231,
- MeSH
- bakteriociny biosyntéza izolace a purifikace terapeutické užití MeSH
- Enterococcus faecium fyziologie MeSH
- Listeria monocytogenes patologie účinky léků MeSH
- listeriové infekce prevence a kontrola přenos MeSH
- sýr mikrobiologie MeSH
- Publikační typ
- techniky in vitro MeSH
- Publikační typ
- abstrakt z konference MeSH
