Two enterococcal strains LMG 16607(T) and LMG 16612 originating from sea water were analysed in a polyphasic taxonomic study. Both strains, assigned as Enterococcus sp. in the BCCM/LMG culture collection, possessed analogous protein profiles, but these were different from all other enterococcal species. 16S rRNA gene sequence analysis of one strain showed the highest similarity, 96.9-96.1%, with its closest phylogenetic neighbours Enterococcus saccharolyticus, Enterococcus sulfureus, Enterococcus saccharominimus and Enterococcus italicus. Further genomic analysis by (GTG)(5)-PCR fingerprinting and sequence analysis of the housekeeping gene phenylalanyl-tRNA synthase (pheS) and distinct biochemical features confirmed that the two strains represent a novel enterococcal species for which the name Enterococcus aquimarinus sp. nov. is proposed. The type strain is LMG 16607(T) (=CCM 7283(T)).
- MeSH
- DNA bakterií analýza MeSH
- DNA fingerprinting metody MeSH
- Enterococcus genetika chemie izolace a purifikace klasifikace MeSH
- fenotyp MeSH
- fenylalanin-tRNA-ligasa genetika MeSH
- financování organizované MeSH
- fylogeneze MeSH
- geny rRNA MeSH
- molekulární sekvence - údaje MeSH
- mořská voda mikrobiologie MeSH
- ribozomální DNA analýza MeSH
- RNA ribozomální 16S MeSH
- sekvenční analýza DNA MeSH
- techniky typizace bakterií MeSH
- MeSH
- Enterococcus klasifikace MeSH
- Helix (hlemýždi) mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
A polyphasic taxonomic approach was used to study atypical enterococci isolated from surface waters. All strains were characterized by physiological and biochemical tests as well as by genotyping. The results of biochemical tests and tRNA intergenic length polymorphism analysis (tDNA-PCR) divided all studied strains uniformly into two groups. Because these groups were clearly separated from all enterococcal species described to date, 16S rDNA sequence analysis, DNA base composition analysis and DNA-DNA hybridization of representative strains were done to elucidate the taxonomic position of the analysed groups. On the basis of the results obtained, the names Enterococcus haemoperoxidus (type strain CCM 4851T = LMG 19487T) and Enterococcus moraviensis (type strain CCM 4856T = LMG 19486T) are proposed for the two hitherto undescribed species. The type strains and reference cultures have been deposited in the Czech Collection of Microorganisms (CCM), Masaryk University, Brno, Czech Republic, and in the BCCM/LMG Culture Collection, Ghent University, Belgium.
- MeSH
- DNA bakterií chemie genetika MeSH
- druhová specificita MeSH
- Enterococcus klasifikace genetika izolace a purifikace MeSH
- fenotyp MeSH
- fylogeneze MeSH
- genotyp MeSH
- hybridizace nukleových kyselin MeSH
- mikrobiologie vody * MeSH
- molekulární sekvence - údaje MeSH
- ribozomální DNA genetika MeSH
- sekvence nukleotidů MeSH
- terminologie jako téma MeSH
- zastoupení bazí MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- DNA bakterií analýza MeSH
- druhová specificita MeSH
- Enterococcus klasifikace MeSH
- Publikační typ
- srovnávací studie MeSH
