Viable microbial cells are important biocatalysts in the production of fine chemicals and biofuels, in environmental applications and also in emerging applications such as biosensors or medicine. Their increasing significance is driven mainly by the intensive development of high performance recombinant strains supplying multienzyme cascade reaction pathways, and by advances in preservation of the native state and stability of whole-cell biocatalysts throughout their application. In many cases, the stability and performance of whole-cell biocatalysts can be highly improved by controlled immobilization techniques. This review summarizes the current progress in the development of immobilized whole-cell biocatalysts, the immobilization methods as well as in the bioreaction engineering aspects and economical aspects of their biocatalytic applications.
- MeSH
- bioinženýrství * MeSH
- biokatalýza * MeSH
- bioreaktory * MeSH
- imobilizované buňky * MeSH
- lidé MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
This review deals with the main characteristics of modern nanostructures like Au nanoparticles or C nanotubes and the great potential of their application in biosensor manufacture. The principles of biosensing are also mentioned, focusing on the label-free electrochemical detection using self-assembled monolayers on Au surfaces. This review deals mostly with biosensors for use in clinical chemistry and diagnostics as immunosensors. Application of nanoparticles can improve electrode transduction although antigens and antibodies do not form a redox couple.
This review gives the main characteristics of mostly commercially available lectins, including their specificity and practical aspects. Various methods, often routinely used, are described, including manufacture of lectin-based biosensors. Other laboratory techniques, mostly for biomedical purposes (such as glycocode decoding, detection of pathogens and antibodies) and their applications in early diagnostics using the array formats are also mentioned.
- MeSH
- elektronová paramagnetická rezonance metody využití MeSH
- financování organizované MeSH
- imunologické faktory terapeutické užití MeSH
- ionty metabolismus MeSH
- kyselina askorbová analogy a deriváty farmakologie MeSH
- kyselina hyaluronová farmakologie metabolismus MeSH
- lidé MeSH
- měď farmakologie metabolismus MeSH
- penicilamin farmakologie MeSH
- reaktivní formy kyslíku farmakologie metabolismus MeSH
- revmatoidní artritida farmakoterapie MeSH
- volné radikály izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- abstrakty MeSH
- MeSH
- financování organizované MeSH
- Publikační typ
- abstrakty MeSH
- MeSH
- financování organizované MeSH
- Publikační typ
- abstrakty MeSH
- MeSH
- adjuvancia imunologická * MeSH
- antiflogistika nesteroidní MeSH
- chemické techniky analytické MeSH
- glukuronidy MeSH
- glykosaminoglykany MeSH
- imunosupresiva MeSH
- inhibitory angiogeneze MeSH
- kationty dvojmocné * chemie MeSH
- kyselina askorbová * chemie MeSH
- kyselina hyaluronová * chemie MeSH
- viskoelastické doplňky kloubní tekutiny * MeSH
- Publikační typ
- práce podpořená grantem MeSH
- Publikační typ
- abstrakt z konference MeSH
- MeSH
- enzymy imobilizované chemie metabolismus MeSH
- Escherichia coli enzymologie genetika MeSH
- finanční podpora výzkumu jako téma MeSH
- imobilizované buňky enzymologie MeSH
- kyselina cytidinmonofosfát-N-acetylneuraminová metabolismus MeSH
- lyasy oxokyselin MeSH
- oxid křemičitý MeSH
- pektiny chemie MeSH
- Publikační typ
- srovnávací studie MeSH
