AIMS/HYPOTHESIS: Diets rich in n-3 polyunsaturated fatty acids, namely eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), protect against insulin resistance and obesity in rodents and increase insulin sensitivity in healthy humans. We tested whether the anti-diabetic effects of EPA and DHA involve enhanced production of the endogenous insulin sensitiser, adiponectin. METHODS: We studied the effects, in an obesity-promoting high-fat diet, of partial replacement of vegetable oils by EPA/DHA concentrate (6% EPA, 51% DHA) over a 5-week period in adult male C57BL/6J mice that either had free access to food or had their food intake restricted by 30%. At the end of the treatment, systemic markers of lipid and glucose metabolism and full-length adiponectin and leptin were measured. Adiponectin (Adipoq) and leptin (Lep) gene expression in dorsolumbar and epididymal white adipose tissue (WAT) and isolated adipocytes was quantified and adipokine production from WAT explants evaluated. RESULTS: In mice with free access to food, plasma triacylglycerols, NEFA, and insulin levels were lower in the presence of EPA/DHA, while glucose and leptin levels were not significantly altered. Food restriction decreased plasma triacylglycerols, glucose, insulin and leptin, but not adiponectin. EPA/DHA increased plasma adiponectin levels, independent of food intake, reflecting the stimulation of Adipoq expression in adipocytes and the release of adiponectin from WAT, particularly from epididymal fat. Expression of Lep and the release of leptin from WAT, while being extremely sensitive to caloric restriction, was unaltered by EPA/DHA. CONCLUSIONS/INTERPRETATION: Intake of diets rich in EPA and DHA leads to elevated systemic concentrations of adiponectin, largely independent of food intake or adiposity and explain, to some extent, their anti-diabetic effects.

• MeSH
• adiponektin biosyntéza   genetika   krev   MeSH
• aktivace enzymů MeSH
• dietní tuky aplikace a dávkování   farmakologie   MeSH
• financování organizované MeSH
• glukosa metabolismus   MeSH
• inzulin fyziologie   krev   MeSH
• inzulinová rezistence MeSH
• kalorická restrikce MeSH
• kyselina eikosapentaenová aplikace a dávkování   farmakologie   MeSH
• kyseliny dokosahexaenové aplikace a dávkování   farmakologie   MeSH
• leptin analýza   fyziologie   genetika   krev   MeSH
• myši inbrední C57BL MeSH
• myši MeSH
• obezita patofyziologie   prevence a kontrola   MeSH
• polymerázová řetězová reakce s reverzní transkripcí MeSH
• přijímání potravy MeSH
• proteinkinasy metabolismus   MeSH
• regulace genové exprese MeSH
• složení těla MeSH
• triglyceridy krev   MeSH
• tuková tkáň chemie   metabolismus   MeSH
• tukové buňky chemie   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• myši MeSH
• zvířata MeSH