• MeSH
• biologické modely * MeSH
• fyziologie kůže * MeSH
• hojení ran * MeSH
• lidé MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

BACKGROUND: Topical ceramide application is an effective therapeutic approach in skin disorders with disturbed barrier function, including atopic dermatitis and psoriasis. OBJECTIVES: To evaluate ceramide analogue N-tetracosanoyl-(l)-serine tetradecyl ester (14S24) using a novel ex vivo model. METHODS: Freshly excised human skin was disrupted by lipid extraction, tape stripping and sodium lauryl sulphate (SLS) treatment. Barrier perturbation was evaluated by the measurement of transepidermal water loss (TEWL), skin hydration and the penetration of model compound, theophylline (TH), assessed by microdialysis. The effect of topical 5% 14S24 was compared with a commercial formulation containing a skin lipid mixture (LR) and control formulation with no skin lipids (L). RESULTS: Both LR and 14S24 produced significant recovery of TEWL and TH penetration in extracted and tape-stripped skin with 14S24 being significantly more effective. In SLS-treated skin, 14S24 decreased TEWL but not TH penetration; LR was inactive. L improved skin hydration but not barrier characteristics. Weak correlation between TEWL and TH penetration was observed in extracted and tape-stripped skin but not in SLS-treated skin. CONCLUSIONS: Cutaneous microdialysis can serve as a useful tool for the evaluation of skin barrier recovery by topical formulations ex vivo whereas TEWL may not be an appropriate measure of skin barrier function in such studies. The excellent barrier repair activity of 14S24 could be beneficial in skin disorders with ceramide deficiency.

• MeSH
• ceramidy farmakologie   MeSH
• detergenty MeSH
• dodecylsíran sodný MeSH
• dospělí MeSH
• financování organizované MeSH
• kožní absorpce účinky léků   MeSH
• kůže metabolismus   účinky léků   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mikrodialýza metody   MeSH
• perspiratio insensibilis účinky léků   MeSH
• preklinické hodnocení léčiv metody   MeSH
• rozpouštědla MeSH
• serin analogy a deriváty   farmakologie   MeSH
• techniky tkáňových kultur MeSH
• tělesná voda metabolismus   MeSH
• theofylin farmakokinetika   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH