BACKGROUND: CD163 is the monocyte/macrophage receptor for haptoglobin-haemoglobin complexes. The aim of this study was to assess the kinetics in the expression of CD163 on monocytes and the concentration of soluble sCD163 in serum of psoriatic patients in order to examine the effect of Goeckerman therapy. METHODS: sCD163 was measured in 71 patients before and after therapy, and in 57 healthy donors. A subgroup of 40 patients and 25 controls was used to assess the expression of membrane CD163. sCD163 was evaluated by ELISA. Flow cytometry method was used to determine the expression of membrane CD163 on monocytes, expressed as mean fluorescence index (MFI). RESULTS: Before therapy, the serum level of sCD163 was significantly higher in our patients than in controls (P=0.0154). However, we observed a profound decrease in sCD163 in our patients after therapy (P=0.0037). Similar to sCD163, pre-treatment expression of CD163 on monocytes was significantly more enhanced in patients than that in controls (P=0.0078). There was a trend towards down-regulation of the expression after therapy, nonetheless, the change was not statistically significant compared to the values before therapy (P=0.8666). This was also confirmed by comparison with controls which displayed lower expression of CD163 than patients after therapy (P=0.0019). The disease activity, expressed as PASI score, was significantly decreased in our patients by GT (P=0.0001). CONCLUSIONS: While sCD163 level in psoriatic patients was diminished after GT therapy, CD163 expression on monocytes was altered only to a minor extent.

• MeSH
• aktivace makrofágů MeSH
• antigeny diferenciační myelomonocytární krev   fyziologie   MeSH
• antigeny povrchové analýza   MeSH
• aplikace kožní MeSH
• biologické markery MeSH
• CD antigeny krev   fyziologie   MeSH
• dehet uhelný aplikace a dávkování   účinky záření   terapeutické užití   MeSH
• dospělí MeSH
• ELISA MeSH
• fotochemoterapie * MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• monocyty metabolismus   MeSH
• poškození DNA MeSH
• průtoková cytometrie MeSH
• psoriáza krev   farmakoterapie   imunologie   MeSH
• receptory buněčného povrchu krev   fyziologie   MeSH
• rozpustnost MeSH
• stupeň závažnosti nemoci MeSH
• ultrafialové záření MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH