The occurrence of positive synergy between antibiotic discs of amoxicillin/clavulanate and cefoperazone was registered in two Klebsiella pneumoniae strains, isolated from hospitals in Czech and Slovak Republic, indicating the presence of genes coding for an extended-spectrum beta-lactamase active also against cefoperazone, a broad-spectrum cephalosporin. Sulbactam inhibited the hydrolysis of cefoperazone by cell-free lysates of these strains which substantiates its use in combination with cefoperazone. Resistance to cephalothin, cefotaxime, ceftazidime, cefoperazone, cefepime and aztreonam was transferred from K. pneumoniae isolates to Escherichia coli K-12 3110 and to Proteus mirabilis P-38 recipient strains.
- MeSH
- antibakteriální látky farmakologie MeSH
- cefalosporiny farmakologie MeSH
- cefoperazon farmakologie MeSH
- Escherichia coli účinky léků genetika MeSH
- hydrolýza MeSH
- infekce bakteriemi rodu Klebsiella farmakoterapie MeSH
- infekce spojené se zdravotní péčí farmakoterapie MeSH
- Klebsiella pneumoniae účinky léků genetika patogenita MeSH
- kombinace amoxicilinu a kyseliny klavulanové farmakologie MeSH
- lékové interakce MeSH
- lidé MeSH
- mnohočetná léková rezistence * MeSH
- Proteus mirabilis účinky léků genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Slovenská republika MeSH
In this report we describe a specific transfer of carbenicillin and cephaloridine resistance determinants from two different strains of Stenotrophomonas maltophilia: No. 215 and 221 isolated from two critically ill patients treated in different Intensive Care Units of a large University Hospital in Ostrava, Czech Republic. These strains were resistant to flouroquinolones and the following beta-lactam drugs: carbenicillin, cephaloridine, cefotaxime, ceftazidime, cefepime, imipenem, meropenem and aztreonam. Both strains transferred carbenicillin and cephaloridine resistance determinants, with rather different frequency, to Proteus mirabilis P-38. All carbenicillin-selected transconjugants were found by an indirect selection method to be co-resistant to cephaloridine only. In a second cycle of transfers Proteus mirabilis R+ strains directly transferred carbenicillin and cephalothin determinants to Escherichia coli K-12 No. 185 nal+ lac+ recipient strain.
- MeSH
- antibakteriální látky farmakologie MeSH
- antiinfekční látky farmakologie MeSH
- beta-laktamasy analýza metabolismus MeSH
- beta-laktamová rezistence * MeSH
- cefaloridin farmakologie MeSH
- fluorochinolony MeSH
- jednotky intenzivní péče MeSH
- karbenicilin farmakologie MeSH
- kultivační média MeSH
- lidé MeSH
- mnohočetná léková rezistence * MeSH
- Proteus mirabilis účinky léků genetika MeSH
- Xanthomonas účinky léků genetika MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
Six Klebsiella pneumoniae strains were collected from two hospitals in Ostrava, Czech republic. Four strains (Nos. 209, 217, 218, 222) were isolated from sputa of critically ill patients from Municipal Hospital Vítkovice-Ostrava. They were resistant to cephalothin, cefotaxime, and ceftazidime (MIC > 100 mg x l-1). Strain No. 218 was intermediately resistant also to ofloxacin and aztreonam (MIC = 12.5 mg x l-1), strain No. 222 was resistant to aztreonam (MIC = 50 mg x l-1). Determinants of resistance to cephalothin, cefotaxime, aztreonam and ceftazidime were transferred to recipient strains of P. mirabilis P-38 rif+ and E. coli K-12 No. 3110 rif+ by all four strains. Synergy between clavulanate-cefotaxime, clavulanate-ceftazidime and clavulanate-aztreonam indicated production of ESBLs by these strains. Two strains, No. 214 and 224, from patients of the ICU in the University Hospital were resistant to cephalothin, cefotaxime and ceftazidime (MIC > 100 mg x l-1). Strain No. 214 was intermediately resistant to aztreonam and ofloxacin (MIC = 12.5 mg x l-1) and strain No. 224 was highly resistant to aztreonam (MIC = 50 mg x l-1). Synergy between clavulanate and cefotaxime as well as between clavulanate and aztreonam, but not between clavulanate and ceftazidime corresponds with non-transferable ceftazidime resistance in strains No. 214 and 224 and indicates different types of ESBL in strains from each of two hospitals.
- MeSH
- aztreonam farmakologie MeSH
- beta-laktamasy metabolismus MeSH
- beta-laktamová rezistence MeSH
- cefalothin farmakologie MeSH
- cefotaxim farmakologie MeSH
- ceftazidim farmakologie MeSH
- infekce bakteriemi rodu Klebsiella mikrobiologie MeSH
- Klebsiella pneumoniae účinky léků izolace a purifikace MeSH
- lidé MeSH
- nemocnice MeSH
- ofloxacin farmakologie MeSH
- rezistence k cefalosporinům MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- amikacin terapeutické užití MeSH
- dospělí MeSH
- gentamiciny terapeutické užití MeSH
- infekce farmakoterapie mikrobiologie MeSH
- klindamycin terapeutické užití MeSH
- kombinovaná farmakoterapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- linkomycin terapeutické užití MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- MeSH
- farmakoterapie trendy MeSH
- infekce farmakoterapie MeSH
- Publikační typ
- kongresy MeSH
- MeSH
- antibakteriální látky terapeutické užití MeSH
- cefalosporiny terapeutické užití MeSH
- gentamiciny terapeutické užití MeSH
- lidé MeSH
- nemocnice MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- antibakteriální látky terapeutické užití MeSH
- antifungální látky terapeutické užití MeSH
- dítě MeSH
- dospělí MeSH
- lékařská onkologie MeSH
- lidé MeSH
- neutropenie farmakoterapie prevence a kontrola MeSH
- transplantace kostní dřeně MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- lidé MeSH
- Publikační typ
- přehledy MeSH
