Screening and identification of protective antigens are essential for the prevention of infections with Toxoplasma gondii (Nicolle et Manceaux, 1908). In our previous study, T. gondii ribosomal-ubiquitin protein L40 (TgRPL40) was identified as a circulating antigen. However, the function and protective value of TgRPL40 was unknown. In the current study, recombinant TgRPL40 was expressed in Escherichia coli BL21 and antibody was prepared. Western blotting analysis indicated that TgRPL40 was present in circulating antigens and excretory/secretary antigens (ESA). Immunofluorescence and immunoelectron microscopy analysis revealed that TgRPL40 protein is widely distributed in the tachyzoites. Immunisation with recombinant TgRPL40 prolonged the survival of mice infected with tachyzoites. Quantitative real-time polymerase chain reaction analysis showed that immunisation with recombinant TgRPL40 reduced the parasite burden in blood, liver, spleen and brain of mice infected with tachyzoites. These observations indicate that TgRPL40 is a circulating antigen and is an effector of immune protection against acute T. gondii infection.

• MeSH
• antigeny protozoální aplikace a dávkování   MeSH
• faktory virulence aplikace a dávkování   MeSH
• imunizace * MeSH
• myši inbrední ICR MeSH
• myši MeSH
• protozoální proteiny aplikace a dávkování   MeSH
• rekombinantní proteiny aplikace a dávkování   MeSH
• syntetické vakcíny aplikace a dávkování   MeSH
• Toxoplasma imunologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Aim:The aim of this study was to give an overview of family caregiver satisfaction within the home palliative care situation inNorth Rhine-Westphalia, Germany. Design:A cross-sectional study. Methods: An anonymous questionnaire, with seven validated scales, and comprising of 71 items, was used. The items investigated perceived needs and burdens of families withina home-based palliative care situation. The satisfaction of the family caregivers with the services delivered by palliative care teams was measured by the FAMCARE-2 Scale. Descriptive statistics and analysis of covariance (ANCOVA) were performed. Results: A convenience sample of 106 family members agreed to participate in the study. Overall, we found high satisfaction within our sample. There was high satisfaction with how the services respected the dignity of families, and how they providedcomfort to patients. Satisfaction was lower with regardto information about patients. Conclusion:High or low satisfaction with palliative care, tells us little about the quality of services. The high satisfaction within this study could be interpreted as a sign that palliative care was important to families at the time ofavailability. Regular and continuous assessment can serve to inform the continuous quality of care provision for patients and their families.

• Klíčová slova
• spokojenost,
• MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mínění MeSH
• osoby pečující o pacienty * psychologie   statistika a číselné údaje   MeSH
• paliativní péče * metody   MeSH
• průřezové studie MeSH
• průzkumy a dotazníky MeSH
• rodinné ošetřovatelství MeSH
• senioři MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH
• Geografické názvy
• Německo MeSH

Ophidascaris wangi sp. n. collected from the king rat snake Elaphe carinata (Günther) (Serpentes: Colubridae) in China is described using both light and scanning electron microscopy. The new species differs from its congeners in the presence of narrow lateral alae originating a short distance posterior to the base of the ventrolateral lips, its relatively long oesophagus (3.57-4.54 mm long, representing 6.6-7.6% of body length), its short spicules (1.89-2.14 mm long, representing 3.9-4.3% of body length), the number and arrangement of caudal papillae (49-57 pairs in total, arranged as follows: 43-51 pairs precloacal, 2 pairs joined paracloacal and 4 pairs postcloacal), the presence of a particular papilliform medioventral, postcloacal ornamentation and the morphology of the eggs and tip of the female tail. In addition, Ophidascaris najae (Gedoelst, 1916), collected from the king cobra Ophiophagus hannah Cantor (Serpentes: Elapidae) in China, is also redescribed. The morphology of the cervical papillae, labial denticles and phasmids of the female is described for the first time.

• MeSH
• Ascaridoidea klasifikace   ultrastruktura   MeSH
• hadi * MeSH
• infekce hlísticemi řádu Ascaridida epidemiologie   parazitologie   veterinární   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Čína MeSH