Poly(2-alkenyl-2-oxazoline)s are promising functional polymers for a variety of biomedical applications, such as drug delivery systems, peptide conjugates, or gene delivery. In this study, poly(2-isopropenyl-2-oxazoline) (PIPOx) is prepared through free-radical polymerization initiated with azobisisobutyronitrile. Reactive 2-oxazoline units in the side chain support an addition reaction with different compounds containing a carboxylic group, which facilitates the preparation of polymers labeled with two different fluorescent dyes. The cytotoxicities of 2-oxazoline monomers, PIPOx, and fluorescently labeled PIPOx are evaluated in vitro using an 3-(4,5-Dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and ex vivo using a cell proliferation assay with adenosine triphosphate bioluminescence. The cell uptake of labeled PIPOx is used to determine the colocalization of PIPOx with cell organelles that are part of the endocytic pathway. For the first time, it is shown that poly(2-isopropenyl-2-oxazoline) is a biocompatible material and is suitable for biomedical applications; further, its immunomodulative properties are evaluated.
- MeSH
- biokompatibilní materiály chemická syntéza chemie farmakologie MeSH
- buněčná smrt účinky léků MeSH
- buňky 3T3 MeSH
- endocytóza účinky léků MeSH
- fibroblasty cytologie MeSH
- fluorescenční spektrometrie MeSH
- imunomodulace účinky léků MeSH
- konfokální mikroskopie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- organely účinky léků metabolismus MeSH
- oxazoly chemická syntéza chemie farmakologie MeSH
- polymery chemická syntéza chemie farmakologie MeSH
- polypropyleny chemická syntéza chemie farmakologie MeSH
- proliferace buněk účinky léků MeSH
- slezina cytologie MeSH
- viabilita buněk účinky léků MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
