Susceptibility in vitro and trends in resistance to antimicrobials were determined by a dilution micromethod in a group of Actinobacillus pleuropneumoniae, Pasteurella multocida, Mannheimia haemolytica and Escherichia coli isolates from clinical cases of cattle and swine diseases in the Czech Republic from 2007 to 2011. A high susceptibility of pig and cattle respiratory pathogens to antimicrobials was found, with the exception of the moderate prevalence of M. haemolytica resistance to ampicillin. In contrast to respiratory pathogens, low susceptibility of E. coli of pig and cattle isolates to ampicillin and amoxicillin/clavulanic acid was noted. Regarding resistance trends, an increase in levels of resistance among E. coli isolates to ampicillin and amoxicillin/clavulanic acid was identified, but the resistance of respiratory isolates was low, with the exception of M. haemolytica. For the period of 2007-2011, there was a significant and almost continuous increase in sales (compared with population correction unit) of ceftiofur, cefquinome and other beta lactams for pigs. Consumption peaked in 2010. In the case of amoxicillin in combination with clavulanic acid, data showed a significant decrease in sales from 2007 to 2008, followed by a period of fluctuation. In cattle, within the groups of 3rd and 4th generation cephalosporins and for the whole group of other betalactams for the period of 2007-2011, there was a significant and almost continuous increase in sales (compared with population correction unit). Consumption peaked in 2010. In the case of ceftiofur, there was a huge increase noted from 2010. In the case of amoxicillin in combination with betalactamase inhibitor (clavulanic acid) data shows a significant decrease from 2007 to 2008, followed by a period of fluctuation in sales.
- MeSH
- Actinobacillus pleuropneumoniae účinky léků MeSH
- bakteriální léková rezistence genetika MeSH
- beta-laktamy metabolismus MeSH
- Escherichia coli účinky léků MeSH
- infekce bakteriemi čeledi Pasteurellaceae epidemiologie veterinární MeSH
- infekce vyvolané Escherichia coli epidemiologie veterinární MeSH
- inhibitory beta-laktamasy farmakologie MeSH
- Mannheimia haemolytica účinky léků MeSH
- mikrobiální testy citlivosti veterinární MeSH
- nemoci prasat epidemiologie mikrobiologie MeSH
- nemoci skotu epidemiologie mikrobiologie MeSH
- Pasteurella multocida účinky léků MeSH
- prasata MeSH
- prevalence MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
Limited data regarding the susceptibility of Actinobacillus pleuropneumoniae to antimicrobials has been published during recent years. Accordingly, the aim of the present study was to investigate the distribution of MICs for the isolates of A. pleuropneumoniae from diseased pigs in the Czech Republic between 2007 and 2009. A total of 242 isolates were tested for susceptibility to 16 antimicrobial agents by a broth microdilution method. A low degree of resistance was observed for florfenicol (0.8%), amoxicillin and clavulanic acid (0.8%), tilmicosin (1.2%), tiamulin (1.7%) and ampicillin (3.3%), whereas resistance to tetracycline was detected more frequently, 23.9% of isolates. Interestingly, resistance to florfenicol has not yet been reported in any study investigating antimicrobial resistance of A. pleuropneumoniae. By PCR the presence of the floR gene was confirmed in all florfenicol resistant isolates.
- MeSH
- Actinobacillus pleuropneumoniae účinky léků izolace a purifikace MeSH
- amoxicilin farmakologie MeSH
- ampicilin farmakologie MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální geny MeSH
- diterpeny farmakologie MeSH
- kyselina klavulanová farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- nemoci prasat epidemiologie mikrobiologie MeSH
- prasata mikrobiologie MeSH
- tetracyklin farmakologie MeSH
- thiamfenikol analogy a deriváty farmakologie MeSH
- tylosin analogy a deriváty farmakologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
The intensity of antibody responses and level of protection against challenge infection induced by Actinobacillus pleuropneumoniae (APP) were compared in piglets vaccinated intramuscularly with different vaccination doses of a subunit vaccine. Secondary antibody responses to APP exotoxins induced by the intradermally administered diluted vaccines did not differ from those induced by undiluted vaccines administered either intradermally or intramuscularly. The level of protection measured by the clinical course of challenge infection to the extent of lung lesions was significantly higher in animals vaccinated with a three-time diluted vaccine in comparison with animals that were administered intramuscularly or intradermally with the most concentrated or the most diluted vaccines.
- MeSH
- Actinobacillus pleuropneumoniae imunologie patogenita MeSH
- bakteriální vakcíny aplikace a dávkování imunologie MeSH
- imunologická odpověď na dávku MeSH
- infekce bakteriemi rodu Actinobacillus imunologie mikrobiologie patologie prevence a kontrola MeSH
- injekce intradermální MeSH
- nemoci prasat imunologie mikrobiologie patologie prevence a kontrola MeSH
- plíce mikrobiologie patologie MeSH
- prasata MeSH
- protilátky bakteriální krev MeSH
- výsledek terapie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Publikační typ
- abstrakt z konference MeSH
