Proinflammatory cytokines and their inhibitors are involved in the regulation of multiple immune reactions including response to transplanted organs. In this prospective study, we evaluated changes in serum concentrations of six IL-1 family cytokines (IL-1 alpha, IL-1 beta, IL-1RA, IL-18, IL-18BP, and IL-36 beta) in 138 kidney allograft recipients and 48 healthy donors. Samples were collected before transplantation and then after one week, three months and one year, additional sera were obtained at the day of biopsy positive for acute rejection. We have shown, that concentrations of proinflammatory members of the IL-1 family (IL-1β, IL-18, IL-36 β) and anti-inflammatory IL-18BP decreased immediately after the transplantation. The decline of serum IL-1RA and IL-1α was not observed in subjects with acute rejection. IL-18, including specifically its free form, is the only cytokine which increase serum concentrations in the period between one week and three months in both groups of patients without upregulation of its inhibitor, IL-18BP. Serum concentrations of calculated free IL-18 were upregulated in the acute rejection group at the time of acute rejection. We conclude that IL-1 family cytokines are involved mainly in early phases of the response to kidney allograft. Serum concentrations of free IL-18 and IL-18BP represent possible biomarkers of acute rejection, and targeting IL-18 might be of therapeutic value.

• MeSH
• alografty * MeSH
• biologické markery * krev   MeSH
• dospělí MeSH
• homologní transplantace metody   MeSH
• interleukin-1 krev   MeSH
• interleukin-18 * krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mezibuněčné signální peptidy a proteiny krev   MeSH
• prospektivní studie MeSH
• rejekce štěpu * imunologie   krev   MeSH
• transplantace ledvin * MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Peripheral blood monocytes, which serve as precursors for tissue macrophages and dendritic cells (DC), play a key role in the immune response to kidney allograft, reparation processes and homeostasis regulation. In this prospective study, we used multicolor flow cytometry to monitor the phenotypic patterns of peripheral monocytes in subjects with uncomplicated outcomes and those with acute rejection. We found a reciprocal increase in the proportion of "classical monocytes" (CD14+CD16-) along with a decline in pro-inflammatory "intermediary" (CD14+CD16+) and "non-classical" (CD14lowCD16+) monocytes in subjects with normal outcomes. In subjects with acute rejection, we observed no reduction in "intermediary" monocytes and no increase in "classical" monocytes. Patients with uncomplicated outcomes exhibited downregulated HLA-DR in all three monocyte subpopulations. However, non-classical monocytes were unaffected in subjects with acute rejection. Expression of CD47 was downregulated after transplantation, while patients with antibody-mediated rejection and donor-specific antibodies showed higher pre-transplant values. In monocytes isolated at the time of biopsy, CD47 expression was higher in individuals with acute rejection compared to patients with normal outcomes one year post-transplant. Expression of CD209 (DC-SIGN) and the proportion of CD163+CD206+ subpopulations were upregulated during the first week after kidney transplantation. CD209 was also upregulated in samples taken on the day of biopsy confirming acute rejection. Our data demonstrate that kidney allograft transplantation is associated with phenotypic changes in peripheral blood monocytes during acute rejection.

• MeSH
• dospělí MeSH
• fenotyp MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• monocyty patologie   MeSH
• prospektivní studie MeSH
• rejekce štěpu etiologie   patologie   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• transplantace ledvin škodlivé účinky   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

• Publikační typ
• abstrakt z konference MeSH

• MeSH
• bronchiální astma imunologie   MeSH
• bronchiální nemoci MeSH
• imunologické faktory MeSH
• obstrukce dýchacích cest imunologie   MeSH
• zánět MeSH

• MeSH
• alveolární makrofágy imunologie   MeSH
• bronchoalveolární laváž metody   škodlivé účinky   MeSH
• bronchoalveolární lavážní tekutina cytologie   MeSH
• cytodiagnostika MeSH
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• počet buněk MeSH
• poruchy dýchání diagnóza   MeSH
• viabilita buněk MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• mladiství MeSH
• Publikační typ
• srovnávací studie MeSH

• MeSH
• bronchiální astma imunologie   patofyziologie   MeSH
• cytokiny MeSH

• MeSH
• alveolární makrofágy imunologie   MeSH
• antigeny diferenciační myelomonocytární analýza   biosyntéza   MeSH
• bronchoalveolární lavážní tekutina chemie   MeSH
• CD antigeny analýza   biosyntéza   MeSH
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• monocyty imunologie   MeSH
• plicní fibróza imunologie   MeSH
• sarkoidóza imunologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH

• MeSH
• alveolární makrofágy imunologie   MeSH
• bronchoalveolární lavážní tekutina MeSH
• fenotyp MeSH
• plíce imunologie   patofyziologie   MeSH

• MeSH
• makrofágy imunologie   MeSH
• molekuly buněčné adheze MeSH
• plicní alveoly imunologie   MeSH

• MeSH
• alveolární makrofágy imunologie   MeSH
• bronchoalveolární lavážní tekutina imunologie   MeSH
• CD antigeny analýza   MeSH
• imunofenotypizace MeSH
• kouření škodlivé účinky   MeSH
• lidé MeSH
• plicní nemoci imunologie   MeSH
• Check Tag
• lidé MeSH