Eviscerácia črevných kľučiek je zriedkavá, ale o to závažnejšia komplikácia, ktorá sa môže vyskytnúť po gynekologických operáciách. Táto kazuistika podrobne popisuje prípad 45-ročnej ženy, u ktorej došli k eviscerácii črevných kľučiek jeden rok po totálnej laparoskopickej hysterektómii s obojstrannou salpingektómiou. Eviscerácii vznikla spontánne v čase použitia toalety za účelom vyprázdnenia stolice. Pacientka nemala anamnézu žiadnej predchádzajúcej brušnej operácie a ani žiadne identifikované rizikové faktory, ako je napríklad recentný pohlavný styk alebo vaginálne vyšetrenie. Tento prípad podčiarkuje dôležitosť adekvátnej a precíznej sutúry pošvového pahýľa po hysterektómii a zdôrazňuje nepredvídateľnú povahu oneskorenej eviscerácie čreva.
Bowel evisceration is a rare but severe complication that can occur following gynecological surgeries. This report details the case of a 45-year-old woman who experienced bowel evisceration one year after undergoing a total laparoscopic hysterectomy with bilateral salpingectomy. The evisceration occurred spontaneously at the time of using the toilet for the purpose of emptying the stool. The patient had no prior surgical abdominal history and no identified risk factors such as recent sexual intercourse or vaginal examination. This case underscores the importance of meticulous vaginal cuff closure during any form of hysterectomy and highlights the unpredictable nature of delayed bowel evisceration.
BACKGROUND/AIM: We have previously shown that the water extract of Agrimonia eupatoria L. (AE) is a valuable source of polyphenols with excellent antioxidant properties and has clinical potential for the prevention and/or adjuvant therapy of cardiovascular complications associated with diabetes. Inspired by our previously published data, in the present study we examined whether AE improves skin wound healing in a series of in vitro and in vivo experiments. MATERIALS AND METHODS: In detail, we investigated the ability of the AE extract to induce fibroblast to myofibroblast conversion, extracellular matrix (ECM) deposition, and keratinocyte proliferation/differentiation, in vitro. In parallel, in an animal model, we measured wound tensile strength (TS) and assessed the progression of open wounds using basic histology and immunofluorescence. RESULTS: The AE extract induced the myofibroblast-like phenotype and enhanced ECM deposition, both in vitro and in vivo. Furthermore, the wound TS of skin incisions and the contraction rates of open excisions were significantly increased in the AE-treated group. CONCLUSION: The present data show that AE water extract significantly improves the healing of open and sutured skin wounds. Therefore, our data warrant further testing in animal models that are physiologically and evolutionarily closer to humans.
Understanding the molecular and cellular processes in skin wound healing can pave the way for devising innovative concepts by turning the identified natural effectors into therapeutic tools. Based on the concept of broad‐scale engagement of members of the family of galactoside‐binding lectins (galectins) in pathophysiological processes, such as cancer or tissue repair/regeneration, the present study investigated the potential of galectins‐1 (Gal‐1) and ‐3 (Gal‐3) in wound healing. Human dermal fibroblasts, which are key cells involved in skin wound healing, responded to galectin exposure (Gal‐1 at 300 or Gal‐3 at 600 ng/ml) with selective changes in gene expression among a panel of 84 wound‐healing‐related genes, as well as remodeling of the extracellular matrix. In the case of Gal‐3, positive expression of Ki67 and cell number increased when using a decellularized matrix produced by Gal‐3‐treated fibroblasts as substrate for culture of interfollicular keratinocytes. In vivo wounds were topically treated with 20 ng/ml Gal‐1 or ‐3, and collagen score was found to be elevated in excisional wound repair in rats treated with Gal‐3. The tensile strength measured in incisions was significantly increased from 79.5±17.5 g/mm2 in controls to 103.1±21.4 g/mm2 after 21 days of healing. These data warrant further testing mixtures of galectins and other types of compounds, for example a combination of galectins and TGF‐β1.
- MeSH
- fibroblasty metabolismus patologie MeSH
- galektiny biosyntéza MeSH
- kolagen biosyntéza MeSH
- krevní proteiny biosyntéza MeSH
- lidé MeSH
- pevnost v tahu * MeSH
- rány a poranění metabolismus patologie MeSH
- regulace genové exprese * MeSH
- škára metabolismus patologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Petitova lumbálna hernia je raritný defekt brušnej steny lokalizovaný v oblasti dolného bedrového trojuholníka. Asi u 9 % pacientov s herniou v oblasti trigonum Petiti sa klinický stav skomplikuje inkarceráciou, najčastejšie hrubého čreva. Autori prezentujú kazuistiku 73 ročného pacienta prijatého a urgentne operovaného pre známky ileózneho stavu. Peroperačným nálezom bola inkarcerovaná ľavostranná lumbálna hernia v oblasti trigonum Petiti. Obsahom herniálneho vaku bola časť colon sigmoideum bez známok porušenej vitality. U pacienta bola vykonaná repozícia colon sigmoideum a plastika bránky lumbálnej hernie. Pooperačný priebeh bol bez komplikácií a pacient bol prepustený do domáceho liečenia 9. po-operačný deň. Predložená práca poukazuje na lumbálnu herniu v oblasti trigonum Petiti ako jednu z menej častých, avšak možných príčin vzniku ileózneho stavu. Taktiež poukazuje na potrebu včasnej diagnostiky inkarcerovanej lumbálnej hernie a jej urgentnú operačnú liečbu.
Petit's hernia is a rare defect in the abdominal wall located in the lower lumbar triangle. The herniation in the Petiti trigonum is complicated by bowel incarceration in about 9% of cases, most often of the large bowel. We present a case report of a 73-year-old patient with clinical manifestations of large bowel obstrution. The patient was admitted and indicated for urgent surgery. Incarcerated left lumbar hernia in the Petit's triangle with herniation of sigmoid colon that showed no signs of ischemia was found. Reposition of the sigmoid colon and hernioplasty of the lumbar hernia was performed. There were no postoperative complications and the patient was discharged on postoperative day 9. The presented case report shows that the lumbar hernia (Petit) may result in bowel obstruction and thus may cause a life-threatening condition that requires urgent surgical intervention.
- Klíčová slova
- lumbální kýla,
- MeSH
- hernie * komplikace MeSH
- ileus * chirurgie etiologie MeSH
- lidé MeSH
- lumbosakrální krajina patologie MeSH
- operace kýly metody MeSH
- senioři MeSH
- výsledek terapie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- Publikační typ
- kazuistiky MeSH
Estrogen deprivation is considered responsible for many age-related processes, including poor wound healing. Guided by previous observations that estradiol accelerates re‑epithelialization through estrogen receptor (ER)‑β, in the present study, we examined whether selective ER agonists [4,4',4''-(4-propyl [1H] pyrazole-1,3,5-triyl)‑trisphenol (PPT), ER‑α agonist; 2,3-bis(4-hydroxyphenyl)-propionitrile (DPN), ER‑β agonist] affect the expression of basic proliferation and differentiation markers (Ki‑67, keratin‑10, ‑14 and ‑19, galectin‑1 and Sox‑2) of keratinocytes using HaCaT cells. In parallel, ovariectomized rats were treated daily with an ER modulator, and wound tissue was removed 21 days after wounding and routinely processed for basic histological analysis. Our results revealed that the HaCaT keratinocytes expressed both ER‑α and ‑β, and thus are well-suited for studying the effects of ER agonists on epidermal regeneration. The activation of ER‑α produced a protein expression pattern similar to that observed in the control culture, with a moderate expression of Ki‑67 being observed. However, the activation of ER‑β led to an increase in cell proliferation and keratin‑19 expression, as well as a decrease in galectin‑1 expression. Fittingly, in rat wounds treated with the ER‑β agonist (DPN), epidermal regeneration was accelerated. In the present study, we provide information on the mechanisms through which estrogens affect the expression patterns of selected markers, thus modulating keratinocyte proliferation and differentiation; in addition, we demonstrate that the pharmacological activation of ER-α and -β has a direct impact on wound healing.
- MeSH
- alfa receptor estrogenů agonisté metabolismus MeSH
- beta receptor estrogenů agonisté metabolismus MeSH
- buněčná diferenciace účinky léků MeSH
- buněčné linie MeSH
- fenoly farmakologie MeSH
- hojení ran účinky léků MeSH
- keratinocyty cytologie účinky léků metabolismus patologie MeSH
- kůže účinky léků metabolismus patologie MeSH
- lidé MeSH
- nitrily farmakologie MeSH
- potkani Sprague-Dawley MeSH
- pyrazoly farmakologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Reactive oxygen species (ROS) are highly considered in the ethiopathogenesis of different pathological conditions because they may cause significant damage to cells and tissues. In this paper, we focused on potential antioxidant properties of two medical plants such as the Agrimonia eupatoria L. and Cynara cardunculus L. Both plants have previously been studied for their pharmacological activities, especially as hepatoprotective and hypoglycemic activities. It has been suggested, that their effects are related to the antioxidant properties of polyphenols, which are dominant compounds of the plants' extracts. In the present study HPLC-MS analysis of water infusion was performed allowing the identification of several phenolic constituents. Furthermore, antioxidant effects of the two extracts were compared showing higher effects for agrimony extract compared to artichoke. Thus, agrimony was selected for the in vivo study using the skin flap viability model. In conclusion, our results provide evidence that the A. eupatoria extract may be a valuable source of polyphenols to be studied for the future development of supplements useful in the prevention of diseases linked to oxidative stress.
- MeSH
- Agrimonia chemie MeSH
- antioxidancia chemie farmakologie MeSH
- buněčné linie účinky léků metabolismus MeSH
- Cynara chemie MeSH
- fenoly chemie farmakologie MeSH
- katalasa metabolismus MeSH
- krysa rodu rattus MeSH
- lidé MeSH
- oxidační stres účinky léků MeSH
- poškození DNA účinky léků MeSH
- reaktivní formy kyslíku metabolismus MeSH
- rostlinné extrakty chemie farmakologie MeSH
- superoxiddismutasa metabolismus MeSH
- viabilita buněk účinky léků MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
We investigated whether early postnatal over-nutrition affects normal course of skin wound healing. To induce over-nutrition the litter size was adjusted on the first day after birth to four pups/nest (small litters). In parallel, as a control, normal nests of 10 pups/nest (normal litters) were used. For the wound healing experiment 30 male Sprague-Dawley rats, 15 from normal nests and 15 from small nests, were used. Two parallel full-thickness skin incisions and two full-thickness excisions were performed on the back of each rat. Samples for histological examination (excisions) and wound tensile strength measurement (incisions) were collected on days 2, 6, and 14 after surgery. Our study demonstrates that rats from the small nests had enhanced plasma levels of insulin and enhanced body weight/fat parameters. Furthermore, in small nests, rats that expressed the above-mentioned symptoms displayed slight improvement of epidermis regeneration, accelerated demarcation line formation, and increased wound tensile strength. From this point of view the small nest model used in the present experiment is helpful for exploration whether these acquired changes might be considered as a sufficient essential factor involved in the regulation of metabolic homeostasis and wound repair in juvenile obese male rats. Nevertheless, further studies need to be performed to verify the present findings also on other animal models and humans and to describe the exact underlying mechanism.
- MeSH
- hojení ran * MeSH
- homeostáza MeSH
- inzulin metabolismus MeSH
- krysa rodu rattus MeSH
- kůže patologie MeSH
- metabolický syndrom genetika MeSH
- modely nemocí na zvířatech MeSH
- novorozená zvířata MeSH
- obezita genetika MeSH
- odstavení MeSH
- pevnost v tahu MeSH
- potkani Sprague-Dawley MeSH
- tělesná hmotnost MeSH
- věda o výživě zvířat MeSH
- velikost vrhu MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
BACKGROUND AND OBJECTIVE: Despite the fact that the molecular mechanism of low-level laser therapy (LLLT) is not yet known, the exploitation of phototherapy in clinical medicine and surgery is of great interest. The present study investigates the effects of LLLT on open skin wound healing in normal and diabetic rats. MATERIALS AND METHODS: Four round full-thickness skin wounds on dorsum were performed in male adult nondiabetic (n = 24) and diabetic (n = 24) Sprague-Dawley rats. AlGaInP (635 nm, wavelength; 5 J/cm(2), daily dose) was used to deliver power densities of 1, 5, and 15 mW/cm(2) three times daily until euthanasia. RESULTS: PMNL infiltration was lower in the irradiated groups (15 mW/cm(2)). The synthesis and organisation of collagen fibres were consecutively enhanced in the 5 mW/cm(2) and 15 mW/cm(2) groups compared to the others in nondiabetic rats. In the diabetic group the only significant difference was recorded in the ratio PMNL/Ma at 15 mW/cm(2). A significant difference in the number of newly formed capillaries in the irradiated group (5, 15 mW/cm(2)) was recorded on day six after injury compared to the control group. CONCLUSION: LLLT confers a protective effect against excessive inflammatory tissue response; it stimulates neovascularization and the early formation of collagen fibres.
- MeSH
- dávka záření MeSH
- experimentální diabetes mellitus patologie terapie MeSH
- fototerapie * MeSH
- hojení ran účinky záření MeSH
- krysa rodu rattus MeSH
- kůže zranění účinky záření MeSH
- laserová terapie s nízkou intenzitou světla * MeSH
- lidé MeSH
- nemoci imunitního systému MeSH
- poruchy leukocytů MeSH
- zánět patologie terapie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
