Transmission of phytoplasmas from naturally infected plant host species using the parasitic plant Cuscuta spp. (dodder) to Catharanthus roseus (Madagascar periwinkle) is an effective way to maintain a wide range of phytoplasmas for further research. Here, we describe transmission via dodder from an infected medicinal plant Rehmannia glutinosa var. purpurea and from a symptomatic redcurrant plant (Ribes spp.) to C. roseus indicator plants using a "stable bridges" method. In both cases, typical symptoms of phytoplasma disease on periwinkle plants were obtained: virescent flowers with an increased number of axillary shoots and smaller leaves after transmission from R. glutinosa, and greening petals (virescence) after transmission from Ribes spp. Phytoplasmas could be detected in donor and recipient plants by electron microscopy and by polymerase chain reaction (PCR) assays using universal phytoplasma primer pairs. Restriction fragment length polymorphism analyses of PCR fragments can also be used to confirm the identity of the phytoplasmas from donor and recipient plants.
Cuscuta europaea and Cuscuta campestris (Cuscutaceae) are annual parasitic plants which wrap around host plants and penetrate their tissues using haustoria. There are more than 150 species of Cuscuta worldwide; C. europaea and C. campestris are the most common species in the Czech Republic and Slovakia. In this study phenolics were identified in methanolic extracts of these species by HPLC and MS. Among others, caffeic acid, p-coumaric acid, isorhamnetin and isorhamnetin 3-O- -glucoside were found in the extracts.
- MeSH
- arginin farmakologie imunologie účinky léků MeSH
- Cuscuta imunologie účinky léků MeSH
- erektilní dysfunkce terapie MeSH
- Ginkgo biloba imunologie účinky léků MeSH
- inzerce jako téma etika metody zákonodárství a právo MeSH
- kontrola potravin metody zákonodárství a právo MeSH
- lidé MeSH
- potravní doplňky škodlivé účinky využití MeSH
- sexuologie metody MeSH
- ženšen imunologie účinky léků MeSH
- Check Tag
- lidé MeSH
