During high throughput sequencing (HTS) of leaves from a symptomatic red clover plant, a new RNA virus, tentatively named red clover nepovirus A (RCNVA), was discovered. The complete genomic sequence was determined and characterized. Particularly noteworthy was that RCNVA shares high sequence identities in RNA1 with a group of phylogenetically related nepoviruses while homologies in the RNA2 segments are markedly lower. Based on the genomic organization and phylogenetic attributes, RCNVA should be classified as a novel virus of the genus Nepovirus (subfamily Comovirinae, family Secoviridae, order Picornavirales).

• MeSH
• Phylogeny MeSH
• Genome, Viral * MeSH
• Plant Diseases virology   MeSH
• Nepovirus classification   genetics   isolation & purification   MeSH
• RNA, Viral genetics   MeSH
• Trifolium virology   MeSH
• Viral Proteins genetics   MeSH
• High-Throughput Nucleotide Sequencing MeSH
• Publication type
• Journal Article MeSH

• MeSH
• Research Support as Topic MeSH
• Plant Diseases virology   MeSH
• Nepovirus genetics   isolation & purification   MeSH
• Rhabdoviridae genetics   isolation & purification   MeSH
• Virion genetics   isolation & purification   MeSH