Most anesthetics induce characteristic hemodynamic changes leading to blood pressure (BP) reduction but the role of renin-angiotensin system (RAS), sympathetic nervous system (SNS) and nitric oxide (NO) synthesis in this BP reduction is unknown. We therefore studied the influence of four widely used anesthetics - pentobarbital (P), isoflurane (ISO), ketamine-xylazine (KX) and chloralose-urethane (CU) - on the participation of these vasoactive systems in BP maintenance. BP effects elicited by the acute sequential blockade of RAS (captopril), SNS (pentolinium) and NO synthase (L-NAME) were compared in conscious and anesthetized Wistar or spontaneously hypertensive rats (SHR). Except for pentobarbital all studied anesthetics evidenced by diminished BP responses to pentolinium. The absolute pentolinium-induced BP changes were always greater in SHR than Wistar rats. KX anesthesia eliminated BP response to pentolinium and considerably enhanced BP response to NO synthase inhibition in SHR. In both rat strains the anesthesia with ISO or CU augmented BP response to captopril, decreased BP response to pentolinium and attenuated BP response to NO synthase inhibition. In conclusion, pentobarbital anesthesia had a modest influence on BP level and its maintenance by the above vasoactive systems. Isoflurane and chloralose-urethane anesthesia may be used in cardiovascular experiments if substantial BP decrease due to altered contribution of RAS, SNS and NO to BP regulation does not interfere with the respective research aim. Major BP reduction (namely in SHR) due to a complete SNS absence is a major drawback of ketamine-xylazine anesthesia.
- MeSH
- anestetika farmakologie MeSH
- chloralosa farmakologie MeSH
- ethyl-karbamát farmakologie MeSH
- gangliové blokátory farmakologie MeSH
- hypertenze metabolismus patofyziologie MeSH
- inhibitory ACE farmakologie MeSH
- inhibitory enzymů farmakologie MeSH
- isofluran farmakologie MeSH
- ketamin farmakologie MeSH
- kombinace anestetik farmakologie MeSH
- krevní tlak účinky léků MeSH
- krysa rodu rattus MeSH
- modely nemocí na zvířatech MeSH
- oxid dusnatý farmakologie MeSH
- pentobarbital farmakologie MeSH
- potkani inbrední SHR MeSH
- potkani Wistar MeSH
- renin-angiotensin systém účinky léků MeSH
- sympatický nervový systém účinky léků patofyziologie MeSH
- synthasa oxidu dusnatého antagonisté a inhibitory metabolismus MeSH
- xylazin farmakologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- MeSH
- chloralosa farmakologie MeSH
- lidé MeSH
- pentobarbital farmakologie MeSH
- sluch MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- MeSH
- anestezie MeSH
- chloralosa MeSH
- elektrická stimulace MeSH
- evokované potenciály MeSH
- mozek MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
