The decomposition of wood and detritus is challenging to most macroscopic organisms due to the recalcitrant nature of lignocellulose. Moreover, woody plants often protect themselves by synthesizing toxic or nocent compounds which infuse their tissues. Termites are essential wood decomposers in warmer terrestrial ecosystems and, as such, they have to cope with high concentrations of plant toxins in wood. In this paper, we evaluated the influence of wood age on the gut microbial (bacterial and fungal) communities associated with the termites Reticulitermes flavipes (Rhinotermitidae) (Kollar, 1837) and Microcerotermes biroi (Termitidae) (Desneux, 1905). We confirmed that the secondary metabolite concentration decreased with wood age. We identified a core microbial consortium maintained in the gut of R. flavipes and M. biroi and found that its diversity and composition were not altered by the wood age. Therefore, the concentration of secondary metabolites had no effect on the termite gut microbiome. We also found that both termite feeding activities and wood age affect the wood microbiome. Whether the increasing relative abundance of microbes with termite activities is beneficial to the termites is unknown and remains to be investigated. IMPORTANCE Termites can feed on wood thanks to their association with their gut microbes. However, the current understanding of termites as holobiont is limited. To our knowledge, no studies comprehensively reveal the influence of wood age on the termite-associated microbial assemblage. The wood of many tree species contains high concentrations of plant toxins that can vary with their age and may influence microbes. Here, we studied the impact of Norway spruce wood of varying ages and terpene concentrations on the microbial communities associated with the termites Reticulitermes flavipes (Rhinotermitidae) and Microcerotermes biroi (Termitidae). We performed a bacterial 16S rRNA and fungal ITS2 metabarcoding study to reveal the microbial communities associated with R. flavipes and M. biroi and their impact on shaping the wood microbiome. We noted that a stable core microbiome in the termites was unaltered by the feeding substrate, while termite activities influenced the wood microbiome, suggesting that plant secondary metabolites have negligible effects on the termite gut microbiome. Hence, our study shed new insights into the termite-associated microbial assemblage under the influence of varying amounts of terpene content in wood and provides a groundwork for future investigations for developing symbiont-mediated termite control measures.

• MeSH
• Bacteria genetika   MeSH
• dřevo * metabolismus   MeSH
• ekosystém MeSH
• Isoptera * mikrobiologie   MeSH
• RNA ribozomální 16S genetika   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The increase in farm plot size is one of the most apparent and significant trends that have influenced central and eastern European agricultural landscapes since the 1950s. In many countries where the average plot size in traditional land-use systems did not exceed several hectares, present-day plots reach the size of 200 ha or more. In recent times, efforts have been made to reverse this trend to restore important ecosystem functions and to re-establish the aesthetic values of everyday landscapes. Visual landscape quality is becoming a major driving force in the development of agricultural landscapes with known effects on people's well-being and health, and this quality plays an increasingly important role in agricultural policies. However, no comprehensive research has been carried out to establish the links between perceived visual landscape quality and the scale of the farm plot pattern. The current study was therefore designed to determine whether greater farmland pattern heterogeneity, i.e., smaller farm plot sizes, is consistent with higher visual preferences. The results showed that people preferred a small-scale plot pattern in landscapes characterized by a flat relief and a low proportion of woody vegetation. These homogeneous landscapes were also overall considered significantly less beautiful than more diverse landscapes. However, even a moderate decrease in plot size notably improved these low beauty scores. These preferences were displayed consistently by all respondents, and most strongly by older respondents, respondents with a higher level of education, and those professionally engaged in landscape design or conservation. The high level of consensus among respondents in rejecting further land consolidation in homogeneous landscapes, which form a large proportion of European farmland, underlines that the results of this study provide a valid argument for discussing sustainable agricultural plot sizes as part of agricultural policy-making.

• MeSH
• dřevo MeSH
• ekosystém * MeSH
• farmy MeSH
• lidé MeSH
• vytváření politiky MeSH
• zachování přírodních zdrojů metody   MeSH
• zemědělství * metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Organic inclusions in lime binders provide useful samples for radiocarbon dating of historical objects. Two Czech castles Týřov and Pyšolec from Late Middle Ages were explored, and tens of charcoals were found in their walls. The radiocarbon content of the charcoals was measured with accelerator mass spectrometry. The dating results showed that none of the charcoals were younger than the known historical ages (Týřov: 1260 - 1270, Pyšolec: 1300 - 1340), but some were considerably older. Two charcoals from Pyšolec castle dated to Palaeolithic, likely originating from fluvial sediments added as an aggregate to the mortar. When excluding these two charcoals, the others indicated most likely dates being 50-100 y older than the building dates of the castles. This systemic effect corresponds to the age of wood used for lime burning and shall be accounted for when dating mortars using charcoals.

• MeSH
• dřevěné a živočišné uhlí * MeSH
• dřevo MeSH
• radioaktivní datování * metody   MeSH
• Publikační typ
• časopisecké články MeSH

Atmospheric carbon dioxide (CO2) has increased substantially since the industrial revolution began, and physiological responses to elevated atmospheric CO2 concentrations reportedly alter the biometry and wood structure of trees. Additionally, soil nutrient availability may play an important role in regulating these responses. Therefore, in this study, we grew 288 two-year-old saplings of sessile oak (Quercus petraea (Matt.) Liebl.) in lamellar glass domes for three years to evaluate the effects of CO2 concentrations and nutrient supply on above- and belowground biomass, wood density, and wood structure. Elevated CO2 increased above- and belowground biomass by 44.3% and 46.9%, respectively. However, under elevated CO2 treatment, sapling wood density was markedly lower (approximately 1.7%), and notably wider growth rings-and larger, more efficient conduits leading to increased hydraulic conductance-were observed. Moreover, despite the vessels being larger in saplings under elevated CO2, the vessels were significantly fewer (p = 0.023). No direct effects of nutrient supply were observed on biomass growth, wood density, or wood structure, except for a notable decrease in specific leaf area. These results suggest that, although fewer and larger conduits may render the xylem more vulnerable to embolism formation under drought conditions, the high growth rate in sessile oak saplings under elevated CO2 is supported by an efficient vascular system and may increase biomass production in this tree species. Nevertheless, the decreased mechanical strength, indicated by low density and xylem vulnerability to drought, may lead to earlier mortality, offsetting the positive effects of elevated CO2 levels in the future.

• MeSH
• biomasa * MeSH
• dřevo růst a vývoj   MeSH
• dub (rod) růst a vývoj   MeSH
• oxid uhličitý MeSH
• půda * MeSH
• stromy růst a vývoj   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Plant lignans possess several properties beneficial for human health and therefore, increasing their contents in foods and beverages is desirable. One of the lignan sources in human diet is wine. To elucidate the origin of lignans contained in wine, LC-MS was used to analyze resinol-related lignans in must, seeds, stems, and wine prepared using stainless steel tanks, oak barrels, and Qvevri (clay vessel). White wines aged in stainless steel tanks contained significantly lower amounts of lignan aglycones (20-60 µg/L) than red and Qvevri wines (300-500 µg/L). Generally, white wines aged in stainless steel tanks contained only low amounts of isolariciresinol and matairesinol. Qvevri wines and red wine aged in stainless steel tank contained up to five lignan compounds and in wine aged in oak barrel, six different lignans were identified. Consistently, only low concentration of isolariciresinol has been found in must, whereas more lignan compounds have been found in grape seeds (isolariciresinol, secoisolariciresinol, and pinoresinol) and stems (isolariciresinol and syringaresinol). Consequently, we conclude that lignan content in wine can be increased by maturation in contact with grape berries, seeds, or stems or with wood.

• MeSH
• dřevo chemie   MeSH
• lignany * analýza   MeSH
• ovoce chemie   MeSH
• víno * analýza   MeSH
• Vitis * MeSH
• Publikační typ
• časopisecké články MeSH

We investigated feasibility of the Full-length complementary DNA OvereXpression (FOX) system as a mutagenesis approach in poplar, using developing xylem tissue. The main goal was to assess the overall mutation rate and if the system will increase instances of mutants affected in traits linked to the xylem tissue. Indeed, we found a high mutation rate of 17.7%, whereas 80% of all mutants were significantly affected in cellulose, lignin and/or hemicellulose. Cell wall biosynthesis is a major process occurring during xylem development. Enrichment of mutants affected in cell wall composition suggests that the tissue source for the FOX library influenced the occurrence of mutants affected in a trait linked to this tissue. Additionally, we found that FLcDNAs from mutants affected in cell wall composition were homologous to genes known to be involved in cell wall biosynthesis and most recovered FLcDNAs corresponded to genes whose native expression was highest in xylem. We characterized in detail a mutant line with increased diameter. The phenotype was caused by a poplar homolog of LONELY GUY 1 (LOG1), which encodes an enzyme in cytokinin biosynthesis and significantly increased xylem proliferation. The causative role of LOG1 in the observed phenotype was further reaffirmed by elevated cytokinin concentration in the mutant and recapitulation overexpression experiment wherein multiple independent lines phenocopied the original FOX mutant. Our experiments show that the FOX approach can be efficiently used for gene discovery and molecular interrogation of traits specific to woody perennial growth and development.

• MeSH
• buněčná stěna genetika   metabolismus   MeSH
• dřevo * genetika   metabolismus   MeSH
• genová knihovna MeSH
• lignin metabolismus   MeSH
• mutační rychlost MeSH
• Populus * genetika   metabolismus   MeSH
• regulace genové exprese u rostlin genetika   MeSH
• rostlinné proteiny genetika   metabolismus   MeSH
• xylém genetika   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH

Brožura se zaměřuje na projekt návrhu stavby bydlení pro seniory, zejména na použité materiály a na konstrukci. Určeno odborné veřejnosti.

• MeSH
• domovy pro seniory MeSH
• dřevo MeSH
• konstrukční materiály MeSH
• projektování a výstavba zařízení metody   MeSH
• zdravotní služby pro seniory MeSH
• železo MeSH
• Publikační typ
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH

• Konspekt
• Architektura
• NLK Obory
• technika
• geriatrie
• NLK Publikační typ
• brožury

BACKGROUND: Genomic selection (GS) or genomic prediction is a promising approach for tree breeding to obtain higher genetic gains by shortening time of progeny testing in breeding programs. As proof-of-concept for Scots pine (Pinus sylvestris L.), a genomic prediction study was conducted with 694 individuals representing 183 full-sib families that were genotyped with genotyping-by-sequencing (GBS) and phenotyped for growth and wood quality traits. 8719 SNPs were used to compare different genomic with pedigree prediction models. Additionally, four prediction efficiency methods were used to evaluate the impact of genomic breeding value estimations by assigning diverse ratios of training and validation sets, as well as several subsets of SNP markers. RESULTS: Genomic Best Linear Unbiased Prediction (GBLUP) and Bayesian Ridge Regression (BRR) combined with expectation maximization (EM) imputation algorithm showed slightly higher prediction efficiencies than Pedigree Best Linear Unbiased Prediction (PBLUP) and Bayesian LASSO, with some exceptions. A subset of approximately 6000 SNP markers, was enough to provide similar prediction efficiencies as the full set of 8719 markers. Additionally, prediction efficiencies of genomic models were enough to achieve a higher selection response, that varied between 50-143% higher than the traditional pedigree-based selection. CONCLUSIONS: Although prediction efficiencies were similar for genomic and pedigree models, the relative selection response was doubled for genomic models by assuming that earlier selections can be done at the seedling stage, reducing the progeny testing time, thus shortening the breeding cycle length roughly by 50%.

• MeSH
• Bayesova věta MeSH
• borovice lesní * genetika   MeSH
• dřevo * genetika   MeSH
• fenotyp MeSH
• genomika MeSH
• jednonukleotidový polymorfismus MeSH
• lidé MeSH
• modely genetické MeSH
• rodokmen MeSH
• šlechtění rostlin MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

Phytophthora cinnamomi is one of the most invasive tree pathogens that devastates wild and cultivated forests. Due to its wide host range, knowledge of the infection process at the molecular level is lacking for most of its tree hosts. To expand the repertoire of studied Phytophthora-woody plant interactions and identify molecular mechanisms that can facilitate discovery of novel ways to control its spread and damaging effects, we focused on the interaction between P. cinnamomi and sweet chestnut (Castanea sativa), an economically important tree for the wood processing industry. By using a combination of proteomics, metabolomics, and targeted hormonal analysis, we mapped the effects of P. cinnamomi attack on stem tissues immediately bordering the infection site and away from it. P. cinnamomi led to a massive reprogramming of the chestnut proteome and accumulation of the stress-related hormones salicylic acid (SA) and jasmonic acid (JA), indicating that stem inoculation can be used as an easily accessible model system to identify novel molecular players in P. cinnamomi pathogenicity.

• MeSH
• cyklopentany metabolismus   MeSH
• dřevo MeSH
• Fagaceae metabolismus   mikrobiologie   MeSH
• homeostáza MeSH
• kořeny rostlin MeSH
• kyselina salicylová metabolismus   MeSH
• metabolomika MeSH
• nemoci rostlin mikrobiologie   MeSH
• oxylipiny metabolismus   MeSH
• Phytophthora patogenita   MeSH
• proteomika MeSH
• regulátory růstu rostlin metabolismus   MeSH
• signální transdukce MeSH
• vazebná místa MeSH
• výpočetní biologie MeSH
• Publikační typ
• časopisecké články MeSH

A woody-biochar was added to waste biomass during a composting process. The resulting compost-char was amended to a metal contaminated soil and two plant species, L. perenne and E. sativa, were grown in a pot experiment to determine 1) plant survival and stress factors, 2) uptake of metals to plants and, 3) chemical characteristics of sampled soils and pore waters. Compost supplemented with biochar after the composting process were also tested, as well as a commercially available compost, for comparison. Co-composting with biochar hastened the composting process, resulting in a composite material of reduced odour, increased maturity, circum-neutral pH and increased moisture retention than compost (increase by 3% of easily removable water content). When amended to the soil, CaCl2 extractable and pore water metals s were reduced by all compost treatments with little influence of biochar addition at any tested dose. Plant growth success was promoted furthest by the addition of co-composted biochar to the test soil, especially in the case of E. sativa. For both tested plant species significant reductions in plant metal concentrations (e.g. 8-times for Zn) were achieved, against the control soil, by compost, regardless of biochar addition. The results of this study demonstrate that the addition of biochar into the composting process can hasten the stability of the resulting compost-char, with more favourable characteristics as a soil amendment/improver than compost alone. This appears achievable whilst also maintaining the provision of available nutrients to soils and the reduction of metal mobility, and improved conditions for plant establishment.

• MeSH
• biodegradace MeSH
• biomasa MeSH
• Brassicaceae chemie   růst a vývoj   MeSH
• dřevěné a živočišné uhlí chemie   MeSH
• dřevo chemie   MeSH
• jílek chemie   růst a vývoj   MeSH
• kompostování * MeSH
• kovy analýza   MeSH
• látky znečišťující půdu analýza   MeSH
• půda chemie   MeSH
• teoretické modely MeSH
• Publikační typ
• časopisecké články MeSH