Stocking rate and rangeland area are key variables to provide the livelihood of herders in different climatic zones. To evaluate the economic and ecological management of pastoral units, this study aims to determine the optimal economic size of pastoral units for livestock grazing use considering the ecological capacity of semi-arid rangelands in different climatic scenarios. Therefore, 12 pastoral units (an area of 47,355 ha) were selected in two climatic zones (summer and winter rangelands) in the Kalat region of Razavi Khorasan Province, Iran. By measuring forage production, carrying capacity was calculated. Based on the results, the costs of traditional livestock management in winter rangelands are higher than those of summer rangelands. Moreover, the current size of the assigned rangeland, especially the summer rangeland, is lower than that of the economic justification. The results emphasize that rangeland-based livestock husbandry cannot create a good livelihood for herders in the region, and it is necessary to pay special attention to other services and aspects, despite the existing ecological and socio-economic complexities. In this regard, providing multi-purpose rangeland use and useable technologies to better manage these areas is necessary to increase per capita household income and reduce the stocking rate in the region's rangelands. Ultimately, both increasing the level of available rangelands and reducing costs by applying new technology are required, as is the economic consideration of pastoral units by using rangelands for multiple purposes.
- MeSH
- Livestock * MeSH
- Seasons MeSH
- Conservation of Natural Resources * MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Iran MeSH
This study was aimed on the detection of methicillin resistant Staphylococcus aureus (MRSA) in different categories of retailed ready-to-eat (RTE) meat products from the Czech producers and determination of their genetic properties, antimicrobial resistance and virulence. In RTE meat products, 2% (4/181) of examined samples were MRSA positive. MRSA strains were detected only in durable fermented meat products made exclusively from pork meat. Detection of livestock-associated MRSA (LA-MRSA) clonal lineages (ST398 and ST4999), SCCmec cassette type V and tetracycline resistance indicate a source of contamination from raw pork. The study confirms the ability of these strains to survive the technological process rather than contamination of meat products from the food processing environment. MRSA strains did not carry any of the tested genes encoding staphylococcal enterotoxins or virulence genes (for Panton-Valentine leukocidin, exfoliative toxins A, B and toxic shock syndrome). Our results point out the spread of LA-MRSA through the meat processing chain.
- MeSH
- Anti-Bacterial Agents pharmacology MeSH
- Livestock MeSH
- Meat Products * MeSH
- Meat MeSH
- Methicillin-Resistant Staphylococcus aureus * genetics MeSH
- Microbial Sensitivity Tests MeSH
- Staphylococcal Infections * MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Geographicals
- Czech Republic MeSH
According to Darwin's theory, endless evolution leads to a revolution. One such example is the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)-Cas system, an adaptive immunity system in most archaea and many bacteria. Gene editing technology possesses a crucial potential to dramatically impact miscellaneous areas of life, and CRISPR-Cas represents the most suitable strategy. The system has ignited a revolution in the field of genetic engineering. The ease, precision, affordability of this system is akin to a Midas touch for researchers editing genomes. Undoubtedly, the applications of this system are endless. The CRISPR-Cas system is extensively employed in the treatment of infectious and genetic diseases, in metabolic disorders, in curing cancer, in developing sustainable methods for fuel production and chemicals, in improving the quality and quantity of food crops, and thus in catering to global food demands. Future applications of CRISPR-Cas will provide benefits for everyone and will save countless lives. The technology is evolving rapidly; therefore, an overview of continuous improvement is important. In this review, we aim to elucidate the current state of the CRISPR-Cas revolution in a tailor-made format from its discovery to exciting breakthroughs at the application level and further upcoming trends related to opportunities and challenges including ethical concerns.
- MeSH
- Archaea metabolism MeSH
- Bacteria metabolism MeSH
- CRISPR-Cas Systems * MeSH
- History, 20th Century MeSH
- History, 21st Century MeSH
- Livestock MeSH
- Gene Editing methods MeSH
- Genetic Engineering history methods MeSH
- Genome MeSH
- Humans MeSH
- Clustered Regularly Interspaced Short Palindromic Repeats MeSH
- Crops, Agricultural genetics MeSH
- Animals MeSH
- Check Tag
- History, 20th Century MeSH
- History, 21st Century MeSH
- Humans MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Historical Article MeSH
- Review MeSH
Mycotoxins are secondary metabolites produced by several species of fungi, including the Fusarium, Aspergillus, and Penicillium species. Currently, more than 300 structurally diverse mycotoxins are known, including a group called minor mycotoxins, namely enniatins, beauvericin, and fusaproliferin. Beauvericin and enniatins possess a variety of biological activities. Their antimicrobial, antibiotic, or ionoforic activities have been proven and according to various bioassays, they are believed to be toxic. They are mainly found in cereal grains and their products, but they have also been detected in forage feedstuff. Mycotoxins in feedstuffs of livestock animals are of dual concern. First one relates to the safety of animal-derived food. Based on the available data, the carry-over of minor mycotoxins from feed to edible animal tissues is possible. The second concern relates to detrimental effects of mycotoxins on animal health and performance. This review aims to summarize current knowledge on the relation of minor mycotoxins to livestock animals.
- MeSH
- Depsipeptides toxicity MeSH
- Livestock MeSH
- Edible Grain toxicity MeSH
- Food Contamination analysis MeSH
- Animal Feed toxicity MeSH
- Mycotoxins * MeSH
- Terpenes MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Review MeSH
Helminth infections are ubiquitous in grazing ruminant production systems, and are responsible for significant costs and production losses. Anthelmintic Resistance (AR) in parasites is now widespread throughout Europe, although there are still gaps in our knowledge in some regions and countries. AR is a major threat to the sustainability of modern ruminant livestock production, resulting in reduced productivity, compromised animal health and welfare, and increased greenhouse gas emissions through increased parasitism and farm inputs. A better understanding of the extent of AR in Europe is needed to develop and advocate more sustainable parasite control approaches. A database of European published and unpublished AR research on gastrointestinal nematodes (GIN) and liver fluke (Fasciola hepatica) was collated by members of the European COST Action "COMBAR" (Combatting Anthelmintic Resistance in Ruminants), and combined with data from a previous systematic review of AR in GIN. A total of 197 publications on AR in GIN were available for analysis, representing 535 studies in 22 countries and spanning the period 1980-2020. Reports of AR were present throughout the European continent and some reports indicated high within-country prevalence. Heuristic sample size-weighted estimates of European AR prevalence over the whole study period, stratified by anthelmintic class, varied between 0 and 48%. Estimated regional (country) prevalence was highly heterogeneous, ranging between 0% and 100% depending on livestock sector and anthelmintic class, and generally increased with increasing research effort in a country. In the few countries with adequate longitudinal data, there was a tendency towards increasing AR over time for all anthelmintic classes in GIN: aggregated results in sheep and goats since 2010 reveal an average prevalence of resistance to benzimidazoles (BZ) of 86%, macrocyclic lactones except moxidectin (ML) 52%, levamisole (LEV) 48%, and moxidectin (MOX) 21%. All major GIN genera survived treatment in various studies. In cattle, prevalence of AR varied between anthelmintic classes from 0-100% (BZ and ML), 0-17% (LEV) and 0-73% (MOX), and both Cooperia and Ostertagia survived treatment. Suspected AR in F. hepatica was reported in 21 studies spanning 6 countries. For GIN and particularly F. hepatica, there was a bias towards preferential sampling of individual farms with suspected AR, and research effort was biased towards Western Europe and particularly the United Kingdom. Ongoing capture of future results in the live database, efforts to avoid bias in farm recruitment, more accurate tests for AR, and stronger appreciation of the importance of AR among the agricultural industry and policy makers, will support more sophisticated analyses of factors contributing to AR and effective strategies to slow its spread.
- MeSH
- Anthelmintics * pharmacology therapeutic use MeSH
- Livestock * parasitology MeSH
- Nematoda * drug effects MeSH
- Goats MeSH
- Drug Resistance * MeSH
- Nematode Infections * drug therapy epidemiology parasitology veterinary MeSH
- Sheep MeSH
- Parasitic Diseases, Animal drug therapy epidemiology parasitology MeSH
- Cattle MeSH
- Animals MeSH
- Check Tag
- Cattle MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Meta-Analysis MeSH
- Geographicals
- Europe MeSH
Livestock diseases caused by Trypanosoma congolense, T. vivax and T. brucei, collectively known as nagana, are responsible for billions of dollars in lost food production annually. There is an urgent need for novel therapeutics. Encouragingly, promising antitrypanosomal benzoxaboroles are under veterinary development. Here, we show that the most efficacious subclass of these compounds are prodrugs activated by trypanosome serine carboxypeptidases (CBPs). Drug-resistance to a development candidate, AN11736, emerged readily in T. brucei, due to partial deletion within the locus containing three tandem copies of the CBP genes. T. congolense parasites, which possess a larger array of related CBPs, also developed resistance to AN11736 through deletion within the locus. A genome-scale screen in T. brucei confirmed CBP loss-of-function as the primary mechanism of resistance and CRISPR-Cas9 editing proved that partial deletion within the locus was sufficient to confer resistance. CBP re-expression in either T. brucei or T. congolense AN11736-resistant lines restored drug-susceptibility. CBPs act by cleaving the benzoxaborole AN11736 to a carboxylic acid derivative, revealing a prodrug activation mechanism. Loss of CBP activity results in massive reduction in net uptake of AN11736, indicating that entry is facilitated by the concentration gradient created by prodrug metabolism.
- MeSH
- Livestock MeSH
- Carboxypeptidases metabolism MeSH
- Carboxylic Acids metabolism MeSH
- Drug Resistance MeSH
- Mice MeSH
- Parasitemia veterinary MeSH
- Prodrugs metabolism MeSH
- Protozoan Proteins metabolism MeSH
- Boron Compounds metabolism MeSH
- Trypanocidal Agents metabolism MeSH
- Trypanosoma brucei brucei drug effects enzymology MeSH
- Trypanosoma congolense drug effects enzymology MeSH
- Trypanosoma vivax drug effects enzymology MeSH
- Trypanosomiasis, African drug therapy parasitology veterinary MeSH
- Valine analogs & derivatives metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
The increasing antibiotic resistance of microbial pathogens isolated from farm animals tissues and the environment has been the one of the most important challenges associated with the use of antibiotics. In order to achieve better production on a farm, animal feed is enriched with antibiotics often originally intended for therapeutic purposes, which may lead to notable increases in microbial resistance. One possible approach to decreasing the excessive use of antibiotics in livestock as well as antimicrobial resistance is utilizing the antimicrobial properties of natural substances. The aim of this study was to evaluate the antimicrobial activity of natural substances including carvacrol, thymol, eugenol, gallic acid, octyl gallate, cnicin and usnic acid against a wide spectrum of microorganisms. Cnicin was the only compound which was isolated from the plant with use of column chromatography. The antimicrobial activities of these natural substances were determined on the basis of their minimum inhibitory, minimum bactericidal and minimum fungicidal concentrations using the microdilution method. This determination of antimicrobial activity revealed thymol and cnicin to be effective natural substances against all tested microorganisms. Octyl gallate had a strong inhibitory and bactericidal effect against gram-positive bacteria and was the most effective against Candida strains. Usnic acid was shown to have the lowest minimum inhibitory concentrations for gram-positive bacteria. These results suggest the possible incorporation of natural substances in animal rearing in order to reduce the high amount of antibiotics which are not used directly to treat animal diseases.
- MeSH
- Drug Resistance, Microbial MeSH
- Antifungal Agents analysis MeSH
- Anti-Infective Agents * analysis MeSH
- Bacteria MeSH
- Candida MeSH
- Breeding MeSH
- Livestock * MeSH
- Indicator Dilution Techniques MeSH
- Culture Techniques MeSH
- Animals MeSH
- Check Tag
- Animals MeSH
- Publication type
- Evaluation Study MeSH
- Research Support, Non-U.S. Gov't MeSH
