The hippocampus is important for learning tasks, such as conditioned place preference (CPP), which is widely used as a model for studying the reinforcing effects of drugs with dependence liability. Long-term opiate use may produce maladaptive plasticity in the brain structures involved in learning and memory, such as the hippocampus. We investigated the phenomenon of conditioning with morphine on the cell density of female rat hippocampus. Forty-eight female Wistar rats weighing on average 200-250 g were used. Rats were distributed into eight groups. Experimental groups received morphine daily (three days) at different doses (2.5, 5, 7.5 mg/kg) and the control-saline group received normal saline (1 ml/kg), and then the CPP test was performed. Three sham groups received only different doses (2.5, 5, 7.5 mg/kg) of morphine without CPP test. Forty-eight hours after behavioural testing animals were decapitated under chloroform anaesthesia and their brains were fixed, and after tissue processing, slices were stained with cresyl violet for neurons and phosphotungstic acid haematoxylin for astrocytes. The maximum response was obtained with 5 mg/kg of morphine. The density of neurons in CA1 and CA3 areas of hippocampus after injection of morphine and CPP was decreased. The number of astrocytes in different areas of hippocampus was increased after injection of morphine and CPP. It seems that the effective dose was 5 mg/kg, as it led to the CPP. We concluded that both injection of mor phine and CPP can decrease the density of neurons and also increase the number of astrocytes in the rat hippocampus.
- MeSH
- astrocyty cytologie účinky léků MeSH
- benzoxaziny chemie MeSH
- chování zvířat MeSH
- hematoxylin chemie MeSH
- hipokampus cytologie účinky léků fyziologie MeSH
- krysa rodu rattus MeSH
- kyselina fosfowolframová chemie MeSH
- lokomoce MeSH
- morfin MeSH
- neurony cytologie účinky léků MeSH
- podmiňování (psychologie) * MeSH
- potkani Wistar MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- aldehydy MeSH
- antisérum MeSH
- arboviry imunologie izolace a purifikace patogenita MeSH
- barvení a značení MeSH
- buněčné linie MeSH
- cytopatogenní efekt virový MeSH
- elektronová mikroskopie MeSH
- hemaglutinační testy MeSH
- klíšťata MeSH
- komplement fixační testy MeSH
- křečci praví MeSH
- kultivace virů MeSH
- kyselina fosfowolframová MeSH
- ledviny MeSH
- morčata MeSH
- mozek MeSH
- myši MeSH
- testy inhibice hemaglutinace MeSH
- ultracentrifugace MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- morčata MeSH
- myši MeSH
- zvířata MeSH
- MeSH
- antibiotická rezistence MeSH
- arboviry klasifikace patogenita účinky léků MeSH
- barvení a značení MeSH
- centrifugace - gradient hustoty MeSH
- cesium MeSH
- chloridy MeSH
- chromatografie MeSH
- elektronová mikroskopie MeSH
- krysa rodu rattus MeSH
- kultivace virů MeSH
- kultivační techniky MeSH
- kyselina fosfowolframová MeSH
- mozek MeSH
- protaminy farmakologie MeSH
- sacharosa MeSH
- streptomycin farmakologie MeSH
- ultrazvuk MeSH
- vibrace MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- MeSH
- barvení a značení MeSH
- detergenty farmakologie MeSH
- dimethylsulfoxid farmakologie MeSH
- elektronová mikroskopie MeSH
- ethylethery farmakologie MeSH
- formamidy farmakologie MeSH
- guanidiny farmakologie MeSH
- hemaglutininy virové MeSH
- hydroxylaminy farmakologie MeSH
- indikátory a reagencie farmakologie MeSH
- kyselina fosfowolframová MeSH
- močovina farmakologie MeSH
- nukleoproteiny MeSH
- Orthomyxoviridae účinky léků MeSH
- virové proteiny MeSH
- žlučové kyseliny a soli farmakologie MeSH
