As the consumption of implants increases, so do the requirements for individual types of implants, for example, improved biocompatibility or longevity. Therefore, the nano-modification of the titanium surface is often chosen. The aim was to characterize the modified surface with a focus on medical applications. The titanium surface was modified by the anodic oxidation method to form nanotubes. Subsequently, the material was characterized and analyzed for medical applications-surface morphology, surface wettability, chemical composition, and release of ions into biological fluids. A human gingival fibroblasts (HGFb) cell line was used in the viability study. A homogeneous layer of nanotubes of defined dimensions was formed on the titanium surface, ensuring the material's biocompatibility-the preparation conditions influence the resulting properties of the nanostructured surface. Nanostructured titanium exhibited more suitable characteristics (e.g., wettability, roughness, ion release) for biological applications than compared to pure titanium. It was possible to understand the behavior of the modified layer on the titanium surface and its effect on cell behavior. Another contribution of this work is the combination of material characterization (ion release) with the study of cytocompatibility (direct contact of cells with metals).
- MeSH
- fibroblasty MeSH
- lidé MeSH
- nanostruktury * MeSH
- povrchové vlastnosti MeSH
- smáčivost MeSH
- titan * farmakologie chemie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- kontaktní čočky * MeSH
- lidé MeSH
- smáčivost * MeSH
- Check Tag
- lidé MeSH
Zein is renewable plant protein with valuable film-forming properties that can be used as a packaging material. It is known that the addition of natural cross-linkers can enhance a film's tensile properties. In this study, we aimed to prepare antimicrobial zein-based films enriched with monolaurin, eugenol, oregano, and thyme essential oil. Films were prepared using the solvent casting technique from ethanol solution. Their physicochemical properties were investigated using structural, morphological, and thermal techniques. Polar and dispersive components were analyzed using two models to evaluate the effects on the surface free energy values. The antimicrobial activity was proven using a disk diffusion method and the suppression of bacterial growth was confirmed via a growth kinetics study with the Gompertz function. The films' morphological characteristics led to systems with uniform distribution of essential oils or eugenol droplets combined with a flat-plated structure of monolaurin. A unique combination of polyphenolic eugenol and amphiphilic monoglyceride provided highly stretchable films with enhanced barrier properties and efficiency against Gram-positive and Gram-negative bacteria, yeasts, and molds. The prepared zein-based films with tunable surface properties represent an alternative to non-renewable resources with a potential application as active packaging materials.
- MeSH
- antibakteriální látky farmakologie MeSH
- antifungální látky farmakologie MeSH
- biomechanika účinky léků MeSH
- diferenciální skenovací kalorimetrie MeSH
- Escherichia coli účinky léků MeSH
- eugenol farmakologie MeSH
- laurany farmakologie MeSH
- mikroskopie atomárních sil MeSH
- monoglyceridy farmakologie MeSH
- obaly potravin * MeSH
- oleje prchavé farmakologie MeSH
- pára MeSH
- permeabilita MeSH
- povrchové vlastnosti MeSH
- smáčivost MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- Staphylococcus aureus účinky léků MeSH
- zein farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
Ultra-low fouling and functionalizable coatings represent emerging surface platforms for various analytical and biomedical applications such as those involving examination of cellular interactions in their native environments. Ultra-low fouling surface platforms as advanced interfaces enabling modulation of behavior of living cells via tuning surface physicochemical properties are presented and studied. The state-of-art ultra-low fouling surface-grafted polymer brushes of zwitterionic poly(carboxybetaine acrylamide), nonionic poly(N-(2-hydroxypropyl)methacrylamide), and random copolymers of carboxybetaine methacrylamide (CBMAA) and HPMAA [p(CBMAA-co-HPMAA)] with tunable molar contents of CBMAA and HPMAA are employed. Using a model Huh7 cell line, a systematic study of surface wettability, swelling, and charge effects on the cell growth, shape, and cytoskeleton distribution is performed. This study reveals that ultra-low fouling interfaces with a high content of zwitterionic moieties (>65 mol%) modulate cell behavior in a distinctly different way compared to coatings with a high content of nonionic HPMAA. These differences are attributed mostly to the surface hydration capabilities. The results demonstrate a high potential of carboxybetaine-rich ultra-low fouling surfaces with high hydration capabilities and minimum background signal interferences to create next-generation bioresponsive interfaces for advanced studies of living objects.
- MeSH
- biokompatibilní potahované materiály * chemie farmakologie MeSH
- cytoskelet metabolismus MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- polymery * chemie farmakologie MeSH
- smáčivost MeSH
- testování materiálů * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Surface of ultra-high-molecular-weight polyethylene (UHMWPE) was modified by chemical methods. Surface was firstly activated by Piranha solution and then grafted with selected amino-compounds (cysteamine, ethylenediamine or chitosan). The next step was grafting of some borane cluster compounds, highly fluorescent borane hydride cluster anti-B18H22 or its thiolated derivative 4,4'-(HS)2-anti-B18H20. Polymer foils were studied using various methods to characterize surface chemistry (X-ray photoelectron spectroscopy), roughness and morphology (atomic force microscopy, scanning electron microscopy), chemistry and polarity (electrokinetic analysis), wettability (goniometry) and photophysical properties (UV-Vis spectroscopy) before and after modification steps. Subsequently some kinds of antimicrobial tests were performed. Immobilization of anti-B18H22 in small quantities onto UHMWPE surface leads to materials with a luminescence. Samples grafted with borane clusters showed significant inhibition of growth for gram-positive bacteria (S. epidermidis). These approaches can be used for (i) luminophores on the base of polymers nanocomposites development and/or (ii) preparation of materials with antimicrobial effects.
In the field of tissue engineering, much research has been devoted to the surface topography of conductive materials. However, less work has been carried out on how the electrical stimulation of such materials influences nerve regeneration. Here, we investigated the effect of electrical stimulation on randomly- and uniaxially-aligned polypyrrole-coated cellulose acetate butyrate (PPy/CAB) nanofibers. First, SEM revealed that the conducting PPy coverage resulted in dramatic changes to the nanofiber morphology. In turn, these changes led to an increase in the sample wettability. Fourier transform spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) confirmed the presence of a PPy layer. Second, human neuroblastoma cells (SH-SY5Y) were seeded on the PPy/CAB nanofibers and stimulated by 100 mV mm-1 at 1 Hz pulses in vitro. We demonstrated that either with or without this electrical stimulation both nanofiber alignment and PPy coverage had a strong influence on cell morphology and attachment. Moreover, fluorescence microscopy revealed that the cells stimulated on PPy/CAB had longer neurite outgrowth. Collectively, our results shed light on the combined effect of scaffold morphology and external stimulation on neuronal cell behavior.
- MeSH
- buněčná adheze účinky léků MeSH
- celulosa analogy a deriváty farmakologie toxicita MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- nanovlákna chemie toxicita MeSH
- neurity účinky léků MeSH
- neuronální růst účinky léků MeSH
- polymery farmakologie toxicita MeSH
- proliferace buněk účinky léků MeSH
- pyrroly farmakologie toxicita MeSH
- smáčivost MeSH
- viabilita buněk účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Radio frequency plasma is one of the means to modify the polymer surface namely in the activation of polypropylene membranes (PPM) with O2 plasma. Activated membranes were deposited with TiO2 nanoparticles by the dip coating method and the bare sample and modified sample (PPM5-TiO2) were irradiated by UV lamps for 20-120 min. Characterization techniques such as X-ray diffraction (XRD), Attenuated total reflection technique- Fourier transform infrared spectroscopy (ATR-FTIR), Thermogravimetric analysis (TGA), X-ray photoelectron spectroscopy (XPS), Scanning electron microscope (SEM) and water contact angle (WCA) measurements were applied to study the alteration of ensuing membrane surface properties which shows the nanoparticles on the sample surface including the presence of Ti on PPM. The WCA decreased from 135° (PPM) to 90° (PPM5-TiO2) and after UV irradiation, the WCA of PPM5-TiO2 diminished from 90° to 40°.
- MeSH
- difrakce rentgenového záření MeSH
- fotoelektronová spektroskopie MeSH
- kyslík chemie MeSH
- membrány umělé * MeSH
- nanočástice chemie MeSH
- plazmové plyny chemie MeSH
- polypropyleny chemie MeSH
- smáčivost MeSH
- spektroskopie infračervená s Fourierovou transformací MeSH
- teplota MeSH
- termogravimetrie MeSH
- titan chemie MeSH
- voda chemie MeSH
- Publikační typ
- časopisecké články MeSH
A dual-mode functional chip for chiral sensing based on mobile phone wettability measurements and portable surface-enhanced Raman spectroscopy (SERS) is reported. The plasmon-active regular gold grating surface was covalently grafted with chiral recognition moieties, l- or d-enantiomers of tartaric acid, making stereoselective discrimination of chiral amines possible. Chiral sensing of amines includes two modes of analysis, performed subsequently on the one chip surface with portable instruments (mobile phone equipped with a camera and developed application (app) Dropangle and a portable Raman spectrometer). First, the wettability changes, caused by enantioselective entrapping of chiral amines, are monitored and analyzed via our mobile phone app, allowing detection of the optical configuration and concentration of enantiomers with 1 order of magnitude accuracy. Second, SERS measurement on the same chip provides information about the chemical structure of entrapped amines and allows calculation of the enantiomeric excess with great accuracy. The applicability of the developed chip is demonstrated on a variety of chiral amines, including tyrosine, cysteine, dopamine (DOPA), and dextromethorphan in analytical solutions and in commercially available DOPA-containing drug. Moreover, we demonstrate that the chips could be regenerated and used repeatedly for at least five cycles.
- MeSH
- kontaktní čočky * MeSH
- lidé MeSH
- modul pružnosti MeSH
- smáčivost MeSH
- Check Tag
- lidé MeSH
As coprocessed excipients (CPE) gain a lot of focus recently, this article compares three commercially available CPE of Avicel brand, namely, CE 15, DG, and HFE 102. Comparison is based on measured physical properties of coprocessed mixtures, respectively, flow properties, pycnometric density, mean particle size, specific surface area, moisture content, hygroscopicity, solubility, pH leaching, electrostatic charge, SEM images, and DSC. Tablets were made employing three pressure sets. Viscoelastic properties and ejection force were assessed during compression, as well as pycnometric density, mass uniformity, height, tensile strength, friability, disintegration, and wetting times. Avicel CE 15 is of mid-range flow properties, contains mid-size and nonspherical particles, and has high hygroscopicity, growing negative charge, best lubricity, lowest tensile strength, and mid-long disintegration times. Avicel DG possesses the worst flow properties, small asymmetrical particles, lowest hygroscopicity, stable charge, intermediate lubricity, and tensile strength and exhibits fast disintegration of tablets. Finally, Avicel HFE 102 has the best flow properties, large symmetrical particles, and middle hygroscopicity and its charge fluctuates throughout blending. It also exhibits inferior lubricity, the highest tensile strength, and slow disintegration of tablets. Generally, it is impossible to select the best CPE, as their different properties fit versatile needs of countless manufacturers and final products.
- MeSH
- časové faktory MeSH
- celulosa chemie MeSH
- diferenciální skenovací kalorimetrie MeSH
- fyzikální absorpce MeSH
- koncentrace vodíkových iontů MeSH
- pevnost v tahu MeSH
- pevnost v tlaku MeSH
- pomocné látky chemie MeSH
- poréznost MeSH
- prášky, zásypy, pudry MeSH
- reologie MeSH
- rozpustnost MeSH
- smáčivost MeSH
- statická elektřina MeSH
- tablety MeSH
- tlak MeSH
- velikost částic MeSH
- vlhkost MeSH
- voda chemie MeSH
- vysoušení * MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
