1. This study evaluated and compared the oxidation status of meat from conventionally-farmed, organic and wild ducks during chilled storage in air or vacuum packaging. In total, 48 carcases from each group of ducks were investigated, and 24 carcases from each type of production were stored under vacuum or in air at 3°C ± 1°C. Sample analyses were conducted after 1, 3, 6 and 9 d of storage.2. The antioxidant capacity (2,2-diphenyl-1-picrylhydrazyl: DPPH), thiobarbituric acid reactive substances (TBARS) and free fatty acids in the breast, thigh and wing muscles were determined.3. The antioxidant capacity of meat from conventional ducks was higher than that from organic and wild ducks. The muscles of organic and wild ducks were more susceptible to secondary oxidation (particularly when stored in air) than conventionally-raised birds. The free fatty acid levels in samples of breast and thigh meat from wild ducks on the last day of storage were higher than in conventional and organic ducks.4. The results showed that the oxidation status of meat from conventional ducks was more stable during refrigerated storage than the meat of organic and wild ducks.
Dehiscence of colorectal anastomosis is a serious complication that is associated with increased mortality, impaired functional and oncological outcomes. The hypothesis was that anastomosis reinforcement and vacuum trans-anal drainage could eliminate some risk factors, such as mechanically stapled anastomosis instability and local infection. Patients with rectal cancer within 10 cm of the anal verge and low anterior resection with double-stapled technique were included consecutively. A stapler anastomosis was supplemented by trans-anal reinforcement and vacuum drainage using a povidone-iodine-soaked sponge. Modified reinforcement using a circular mucosa plication was developed and used. Patients were followed up by postoperative endoscopy and outcomes were acute leak rate, morbidity, and diversion rate. The procedure was successfully completed in 52 from 54 patients during time period January 2019-October 2020. The mean age of patients was 61 years (lower-upper quartiles 54-69 years). There were 38/52 (73%) males and 14/52 (27%) females; the neoadjuvant radiotherapy was indicated in a group of patients in 24/52 (46%). The mean level of anastomosis was 3.8 cm (lower-upper quartiles 3.00-4.88 cm). The overall morbidity was 32.6% (17/52) and Clavien-Dindo complications ≥ 3 grade appeared in 3/52 (5.7%) patients. No loss of anastomosis was recorded and no patient died postoperatively. The symptomatic anastomotic leak was recorded in 2 (3.8%) patients and asymptomatic blind fistula was recorded in one patient 1/52 (1.9%). Diversion ileostomy was created in 1/52 patient (1.9%). Reinforcement of double-stapled anastomosis using a circular mucosa plication with combination of vacuum povidone-iodine-soaked sponge drainage led to a low acute leak and diversion rate. This pilot study requires further investigation.Registered at ClinicalTrials.gov.: Trial registration number is NCT04735107, date of registration February 2, 2021, registered retrospectively.
- MeSH
- anastomóza chirurgická MeSH
- drenáž MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádory rekta * chirurgie MeSH
- netěsnost anastomózy MeSH
- pilotní projekty MeSH
- rektum * chirurgie MeSH
- retrospektivní studie MeSH
- sliznice MeSH
- vakuum MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
Pectus excavatum je najčastejším typom deformity hrudníka. Môže byť asymptomatický, v prípade závažnejších foriem však pacienti môžu pociťovať zvýšenú únavnosť, záťažové dyspnoe, bolesti na hrudníku, palpitácie, prípadne zníženú toleranciu fyzickej námahy. Najčastejšie sú však s touto diagnózou asociované psychické ťažkosti vyplývajúce z vnímania vlastnej deformity ako handicapu, pre ktorý sa pacienti často vyhýbajú aktivitám s odhaleným hrudníkom (napr. plávanie). Jediným typom liečby tejto deformity bola donedávna chirurgická korekcia. Dôležitou súčasťou terapie je rehabilitácia zameraná na nápravu nesprávneho držania tela posilňovaním hrudných a chrbtových svalov, a taktiež na dychové cvičenia. Posledných 15 rokov existuje aj konzervatívna liečba, pomocou tzv. vacuum bellu. Hoci výsledky zahŕňajúce dlhoročné skúsenosti s týmto typom terapie zatiaľ chýbajú, výsledky doterajších štúdií demonštrujú, že liečba vacuum bellom je bezpečným spôsobom liečby pectus excavatum s minimálnym výskytom komplikácií.
Pectus excavatum is the most common type of chest deformity. It is usually asymptomatic, but patients suffering from severe forms may experience increased fatigue, exercise dyspnoea, chest pain, palpitations and/or decreased tolerance to physical activity. Psychological difficulties associated with this diagnosis stem from patients ́ perceiving the deformity as a disability and causing them to avoid activities with an exposed chest (e. g. swimming). For a long time, the only available type of treatment was a surgical correction. An important part of the therapy is rehabilitation aimed at correcting the body posture by strengthening chest and back muscles as well as breathing exercises. In the last 15 years there has been an increase in the use of a conservative treatment using the vacuum bell. Although conclusive results based on long-term supporting evidence are lacking, the disponible studies demonstrate vacuum bell treatment is a safe option for pectus excavatum with minimal complications.
- Klíčová slova
- vacuum bell,
- MeSH
- dítě MeSH
- fyzioterapie (techniky) MeSH
- hrudník vpáčený * rehabilitace terapie MeSH
- konzervativní terapie * MeSH
- lidé MeSH
- vakuum MeSH
- výsledek terapie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
The present study aimed to evaluate the characteristic influence of physical therapeutic procedures of vacuum-compression therapy (VCT) on microvascular perfusion (MiP) and macrovascular perfusion (MaP) of the lower limb in diabetic patients. A sample of nine patients with a medical history of type 2 diabetes was used for the purpose of this study. Most of the subjects' medical conditions included venous and neurological complications of the lower limb, whereas the rest of the subjects entered the treatment due to injury recovery or their phlebological disease. The PeriFlux System 5000 (Perimed, Sweden) diagnostic device was used to measure MiP. The MaP was evaluated based on the perfusion index (PI) using the Extremiter monitoring device (Embitron, Czech Republic) designed to perform VCT procedures. The study found that MiP and MaP increase as an effect of VCT procedures and at the same time PI clearly reflects the effect of the applied vacuum and compression phases, verifying the method's vital influence on peripheral perfusion disorders.
- MeSH
- diabetes mellitus 2. typu komplikace diagnóza patofyziologie MeSH
- diabetická retinopatie MeSH
- dolní končetina patofyziologie MeSH
- lidé MeSH
- perfuze metody MeSH
- vakuum MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The paper discusses the real-time monitoring of the changing sample morphology during the entire lyophilization (freeze-drying) and vacuum-drying processes of model biopharmaceutical solutions by using an environmental scanning electron microscope (ESEM); the device's micromanipulators were used to study the interior of the samples in-situ without exposing the samples to atmospheric water vapor. The individual collapse temperatures (Tc) of the formulations, pure bovine serum albumin (BSA) and BSA/sucrose mixtures, ranged from -5 to -29 °C. We evaluated the impact of the freezing method (spontaneous freezing, controlled ice nucleation, and spray freezing) on the morphologies of the lyophiles at the constant drying temperature of -20 °C. The formulations with Tc above -20 °C resulted in the lyophiles' morphologies significantly dependent on the freezing method. We interpret the observations as an interplay of the freezing rates and directionalities, both of which markedly influence the morphologies of the frozen formulations, and, subsequently, the drying process and the mechanical stability of the freeze-dried cake. The formulation with Tc below -20 °C yielded a collapsed cake with features independent of the freezing method. The vacuum-drying produced a material with a smooth and pore-free surface, where deep cracks developed at the end of the process.
This work focused on the development and validation of the analytical procedure using gas chromatography equipped with vacuum-ultraviolet detection for the specific and sensitive determination of nine photoinitiators in food packages. Subsequently, a comparison of the combination of vacuum ultraviolet spectroscopy with gas chromatography and a developed gas chromatography with mass spectrometry method was performed. The vacuum-ultraviolet spectra of all tested photoinitiators were collected and found to be highly distinct, even for isomers. Under the optimal conditions, the limits of detection for nine photoinitiators ranged from 1 to 5 mg/L using vacuum ultraviolet detection and from 0.15 to 0.5 mg/L using mass spectrometric detection. Both techniques were successfully applied for screening of photoinitiators in seven kinds of food packages and the obtained data showed good agreement (the relative difference was between 3 and 18%). The variability in concentrations found in triplicate samples was assessed to be below 18%. Predominantly benzophenone was found in all analysed samples in the range of 0.31-4.23 mg/kg. It appears to be preferably selected by food packaging manufacturers. This study proposes a new simple and sensitive technique used for analysis of photoinitiators that could be a good alternative to gas chromatography with mass spectrometry.
- MeSH
- benzofenony analýza MeSH
- fotosenzibilizující látky analýza MeSH
- inkoust * MeSH
- kontaminace potravin analýza MeSH
- obaly potravin * MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- spektrofotometrie ultrafialová MeSH
- vakuum MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
The surface conditions on the Moon are extremely harsh with high doses of ultraviolet (UV) irradiation (26.8 W · m-2 UVC/UVB), wide temperature extremes (-171°C to 140°C), low pressure (10-10 Pa), and high levels of ionizing radiation. External spacecraft surfaces on the Moon are generally >100°C during daylight hours and can reach as high as 140°C at local noon. A Lunar Microbial Survival (LMS) model was developed that estimated (1) the total viable bioburden of all spacecraft landed on the Moon as ∼4.57 × 1010 microbial cells/spores at contact, (2) the inactivation kinetics of Bacillus subtilis spores to vacuum as approaching -2 logs per 2107 days, (3) the inactivation of spores on external surfaces due to concomitant low-pressure and high-temperature conditions as -6 logs per 8 h for local noon conditions, and (4) the ionizing radiation by solar wind particles as approaching -3 logs per lunation on external surfaces only. When the biocidal factors of solar UV, vacuum, high-temperature, and ionizing radiation were combined into an integrated LMS model, a -231 log reduction in viable bioburden was predicted for external spacecraft surfaces per lunation at the equator. Results indicate that external surfaces of landed or crashed spacecraft are unlikely to harbor viable spores after only one lunation, that shallow internal surfaces will be sterilized due to the interactive effects of vacuum and thermal cycling from solar irradiation, and that deep internal surfaces would be affected only by vacuum with a degradation rate of -0.02 logs per lunation.
- MeSH
- Bacillus subtilis fyziologie účinky záření MeSH
- biologické modely * MeSH
- kosmická loď MeSH
- kosmické záření škodlivé účinky MeSH
- Měsíc * MeSH
- mikrobiální viabilita účinky záření MeSH
- mimozemské prostředí MeSH
- simulace kosmického prostředí metody MeSH
- spory bakteriální fyziologie účinky záření MeSH
- ultrafialové záření škodlivé účinky MeSH
- vakuum MeSH
- vysoká teplota MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Článek představuje přehled, mechanismus působení, účinnost a spokojenost pacientů s léčbou sexuálních dysfunkcí pomocí mechanických pomůcek.
This article presents the development, mechanism of action, efficacy, and patient satisfaction with regard to vacuum constrictionand external erection devices.
- MeSH
- diabetes mellitus MeSH
- ejakulace MeSH
- erektilní dysfunkce * diagnóza etiologie terapie MeSH
- implantace penilní protézy * metody trendy MeSH
- kardiovaskulární nemoci MeSH
- komorbidita MeSH
- lidé MeSH
- protézy a implantáty dějiny trendy MeSH
- umělé orgány * dějiny trendy MeSH
- vakuum MeSH
- věkové faktory MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
