A 2-year national genomic screening in the Czech Republic identified a notable prevalence of the New Delhi metallo-β-lactamase 5 (NDM-5)-producing Escherichia coli sequence type 38 (ST38) in the city of Brno. Forty-two ST38 E. coli isolates harbored the blaNDM-5 gene on the chromosome. Virulence factors confirmed the persistence of these isolates through biofilm formation. Single Nucleotide Polymorphisms (SNPs)-based phylogeny and CRISPR assay typing showed minimal genomic variations, implying a clonally driven outbreak. Results suggest that this high-risk clone may impose a nationwide problem.

• MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• beta-Lactamases * genetics   MeSH
• Biofilms growth & development   MeSH
• Disease Outbreaks * MeSH
• Escherichia coli * genetics   drug effects   isolation & purification   enzymology   MeSH
• Virulence Factors genetics   MeSH
• Phylogeny MeSH
• Genome, Bacterial MeSH
• Genomics methods   MeSH
• Escherichia coli Infections * microbiology   epidemiology   MeSH
• Polymorphism, Single Nucleotide * MeSH
• Humans MeSH
• Microbial Sensitivity Tests MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Geographicals
• Czech Republic MeSH