Supplementation of the rumen ciliate Diploplastron affine growth medium with commercial chitin stimulated growth of ciliates and the density of their population was positively correlated with chitin doses (r = 0.95; p < 0.01). The cell-free extracts prepared from bacteria-free ciliates degraded chitin to N-acetyl-D: -glucosamine and chitobiose. Three exochitinases, two endochitinases and two beta-N-acetylglucosaminidases were identified in the cell-free extract of protozoa. The molar mass of exochitinases was 80, 65 and 30 kDa, and endochitinases 75 and 50 kDa; the molar mass of one of the identified beta-N-acetylglucosaminidases was 45 kDa.

• MeSH
• bachor metabolismus   parazitologie   MeSH
• chitin metabolismus   MeSH
• chitinasy chemie   klasifikace   metabolismus   MeSH
• Ciliophora enzymologie   růst a vývoj   MeSH
• kultivační média chemie   MeSH
• ovce metabolismus   parazitologie   MeSH
• protozoální proteiny klasifikace   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH

• MeSH
• anaerobní bakterie enzymologie   izolace a purifikace   MeSH
• chitin metabolismus   MeSH
• chitinasy fyziologie   izolace a purifikace   klasifikace   MeSH
• feces mikrobiologie   MeSH
• finanční podpora výzkumu jako téma MeSH
• savci mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH