Lymphogranuloma venereum (LGV), the invasive infection of the sexually transmissible infection (STI) Chlamydia trachomatis, is caused by strains from the LGV biovar, most commonly represented by ompA-genotypes L2b and L2. We investigated the diversity in LGV samples across an international collection over seven years using typing and genome sequencing. LGV-positive samples (n=321) from eight countries collected between 2011 and 2017 (Spain n=97, Netherlands n=67, Switzerland n=64, Australia n=53, Sweden n=37, Hungary n=31, Czechia n=30, Slovenia n=10) were genotyped for pmpH and ompA variants. All were found to contain the 9 bp insertion in the pmpH gene, previously associated with ompA-genotype L2b. However, analysis of the ompA gene shows ompA-genotype L2b (n=83), ompA-genotype L2 (n=180) and several variants of these (n=52; 12 variant types), as well as other/mixed ompA-genotypes (n=6). To elucidate the genomic diversity, whole genome sequencing (WGS) was performed from selected samples using SureSelect target enrichment, resulting in 42 genomes, covering a diversity of ompA-genotypes and representing most of the countries sampled. A phylogeny of these data clearly shows that these ompA-genotypes derive from an ompA-genotype L2b ancestor, carrying up to eight SNPs per isolate. SNPs within ompA are overrepresented among genomic changes in these samples, each of which results in an amino acid change in the variable domains of OmpA (major outer membrane protein, MOMP). A reversion to ompA-genotype L2 with the L2b genomic backbone is commonly seen. The wide diversity of ompA-genotypes found in these recent LGV samples indicates that this gene is under immunological selection. Our results suggest that the ompA-genotype L2b genomic backbone is the dominant strain circulating and evolving particularly in men who have sex with men (MSM) populations.
- MeSH
- Chlamydia trachomatis klasifikace genetika MeSH
- dospělí MeSH
- fylogeneze MeSH
- genomika * MeSH
- genotyp MeSH
- homosexualita mužská MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymphogranuloma venereum epidemiologie mikrobiologie MeSH
- mladý dospělý MeSH
- molekulární epidemiologie * MeSH
- molekulární evoluce * MeSH
- proteiny vnější bakteriální membrány genetika MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza MeSH
- sekvenování celého genomu MeSH
- senioři MeSH
- sexuálně přenosné nemoci mikrobiologie MeSH
- sexuální a genderové menšiny MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Austrálie MeSH
- Evropa MeSH
We are reporting the first case of lymphogranuloma venereum in women in East-Central Europe. A 22-year-old heterosexual woman attended our department of venereology. She complained about a burning sensation in the urethra and vaginal discharge. Many tests were performed, and lymphogranuloma venereum, syphilis, gonorrhea, chlamydial urethritis and cervicitis, genital herpes, genital warts, and hepatitis C were diagnosed. Lymphogranuloma venereum was originally endemic in tropical and subtropical areas, but since 2003, outbreaks of this infection have been reported in North America, Europe, and Australia in men who have sex with men (MSM) community. To date, all cases of lymphogranuloma venereum in the Czech Republic appeared in men, predominantly in HIV-positive MSM. There are not many evidences about lymphogranuloma venereum (LGV) in women in developed countries. This report underlines the need for awareness of lymphogranuloma venereum in women among gynecologists, venereologists, and other physicians not only in Western Europe, but across all European countries.
- MeSH
- Chlamydia trachomatis klasifikace genetika izolace a purifikace MeSH
- dospělí MeSH
- HIV infekce virologie MeSH
- koinfekce mikrobiologie virologie MeSH
- lidé MeSH
- lymphogranuloma venereum mikrobiologie MeSH
- mladý dospělý MeSH
- uretra mikrobiologie MeSH
- vagina mikrobiologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- Chlamydia trachomatis klasifikace růst a vývoj MeSH
- chlamydiové infekce diagnóza terapie MeSH
- lidé MeSH
- sexuálně přenosné nemoci diagnóza klasifikace terapie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
- MeSH
- Chlamydia trachomatis imunologie klasifikace MeSH
- dospělí MeSH
- infekce bakteriemi čeledi Chlamydiaceae epidemiologie komplikace mikrobiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- longitudinální studie MeSH
- nádory děložního čípku epidemiologie etiologie MeSH
- riziko MeSH
- studie případů a kontrol MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- srovnávací studie MeSH
