BACKGROUND: Radiofrequency (RF) and high-intensity focused electromagnetic (HIFEM) technologies are used for noninvasive body shaping as standalone modalities. OBJECTIVE: To examine the effects of novel synchronized RF and HIFEM on subcutaneous adipose tissue in a porcine animal model. MATERIALS AND METHODS: Seven large white pigs aged 6 months received 3 abdominal treatments of simultaneous application of synchronized RF and HIFEM (30 minutes, once per week). Punch biopsies of treated and control subcutaneous tissue were collected at the baseline, 4 days, 2 weeks, 1 month, and 2 months. Specimens were examined by light and scanning electron microscopy. Adipocyte volume was analyzed. Fat tissue temperature was measured in situ (fiber optic probes) and superficially (thermal imager). RESULTS: Fat layer was heated to temperatures of 42 to 45°C. Signs of fat apoptosis (shape alternations and pyknotic nuclei) appeared at day 4 and peaked between 2 weeks and 1 month. Adipocyte volume decreased significantly (p < .001) by 31.1% at 2 weeks, 1 month (-23.6%), and 2 months (-22.0%). Control samples showed healthy adipocytes. Scanning electron microscopy micrographs corroborated histology findings, showing flattened, volume-depleted and disrupted adipocytes. CONCLUSION: Synchronized RF with HIFEM procedure resulted in a significant and sustained fat reduction with no adverse events.
- MeSH
- Body Contouring adverse effects instrumentation methods MeSH
- Microscopy, Electron MeSH
- Combined Modality Therapy instrumentation methods MeSH
- Magnetic Field Therapy adverse effects instrumentation methods MeSH
- Models, Animal MeSH
- Subcutaneous Fat cytology radiation effects ultrastructure MeSH
- Swine MeSH
- Radiofrequency Therapy adverse effects instrumentation methods MeSH
- Adipocytes radiation effects ultrastructure MeSH
- Hot Temperature adverse effects MeSH
- Animals MeSH
- Check Tag
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
Protontherapy is hadrontherapy's fastest-growing modality and a pillar in the battle against cancer. Hadrontherapy's superiority lies in its inverted depth-dose profile, hence tumour-confined irradiation. Protons, however, lack distinct radiobiological advantages over photons or electrons. Higher LET (Linear Energy Transfer) 12C-ions can overcome cancer radioresistance: DNA lesion complexity increases with LET, resulting in efficient cell killing, i.e. higher Relative Biological Effectiveness (RBE). However, economic and radiobiological issues hamper 12C-ion clinical amenability. Thus, enhancing proton RBE is desirable. To this end, we exploited the p + 11B → 3α reaction to generate high-LET alpha particles with a clinical proton beam. To maximize the reaction rate, we used sodium borocaptate (BSH) with natural boron content. Boron-Neutron Capture Therapy (BNCT) uses 10B-enriched BSH for neutron irradiation-triggered alpha particles. We recorded significantly increased cellular lethality and chromosome aberration complexity. A strategy combining protontherapy's ballistic precision with the higher RBE promised by BNCT and 12C-ion therapy is thus demonstrated.
- MeSH
- Alpha Particles therapeutic use MeSH
- Boron chemistry therapeutic use MeSH
- Borohydrides chemistry MeSH
- Cell Death radiation effects MeSH
- Chromosome Aberrations radiation effects MeSH
- Cyclotrons MeSH
- DNA, Neoplasm genetics metabolism radiation effects MeSH
- Fluorescent Dyes chemistry MeSH
- Carbon Isotopes chemistry MeSH
- Karyotyping MeSH
- Combined Modality Therapy instrumentation methods MeSH
- Humans MeSH
- Linear Energy Transfer MeSH
- Cell Line, Tumor MeSH
- Prostatic Neoplasms pathology radiotherapy MeSH
- Neutrons * MeSH
- DNA Damage MeSH
- Proton Therapy * instrumentation methods MeSH
- Relative Biological Effectiveness MeSH
- Sulfhydryl Compounds chemistry MeSH
- Boron Neutron Capture Therapy instrumentation methods MeSH
- Dose-Response Relationship, Radiation MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Chirurgická léčba aneuryzmat a disekcí hrudní aorty stejně jako jejich endovaskulární léčba má své technické i medicínské limity. Cílem této práce je poukázat na možnosti kombinací obou léčebných metod a podělit se o první praktické zkušenosti z jejich provádění, přičemž prováděné výkony dělíme do následujících skupin: – kombinované výkony, kdy chirurgická část umožňuje implantaci stentgraftu do descendentní aorty nebo řeší technicky nedokonalý efekt této implantace, – výkony, kdy kombinací chirurgické a endovaskulární léčby podstatně snížíme operační zátěž především odstraněním nutnosti použití mimotělního oběhu a oběhové zástavy, – výkony, při kterých kombinujeme výhody chirurgické léčby v oblasti aortálního oblouku a endovaskulární léčby v oblasti descendentní aorty. Použití kombinace chirurgických a endovaskulárních technik v léčbě rozsáhlých aneuryzmat či disekcí hrudní aorty se jeví jako perspektivní metoda. Nemocnému může přinést nižší perioperační riziko a nižší pooperační morbiditu. Lze předpokládat dobrý dlouhodobý efekt této léčby, který však bude třeba verifikovat dlouhodobým sledováním nemocných.
The surgical management of aneurysms and dissections of the thoracic aorta, as well as their endovascular management, all have their technical and medical limits. The aim of this work is to point out possibility for combination of the both treatment methods and to share our first practical experience with this procedure. The procedures conducted have been divided into the following groups: – combined procedures, where the surgical part enables implantation of the stentgraft into the descending aorta or is managing imperfect results of such implantations, – procedures, where combining the surgical and endovascular treatment lowers operational stress of the patient, as the extracorporeal circulation and the circulation arrest are not required, – procedures, where advantages of both the surgical treatment in the region of the aortic arch and the endovascular treatment in the region of the descending aorta, are combined. Using the combination of both the surgical and endovascular techniques in the management of the large aneurysms and dissections of the thoracic aorta, appears to be a perspective method. The patient may benefit from its lower perioperative risk rates and lower postoperative morbidity rates. A good long-term effect of the above treatment method may be expected, however, it has to be verified in long-term patient studies.
