Homologous recombination (HR) is essential for maintaining genomic integrity, which is challenged by a wide variety of potentially lethal DNA lesions. Regardless of the damage type, recombination is known to proceed by RAD51-mediated D-loop formation, followed by DNA repair synthesis. Nevertheless, the participating polymerases and extension mechanism are not well characterized. Here, we present a reconstitution of this step using purified human proteins. In addition to Pol δ, TLS polymerases, including Pol η and Pol κ, also can extend D-loops. In vivo characterization reveals that Pol η and Pol κ are involved in redundant pathways for HR. In addition, the presence of PCNA on the D-loop regulates the length of the extension tracks by recruiting various polymerases and might present a regulatory point for the various recombination outcomes.
- MeSH
- DNA-dependentní DNA-polymerasy chemie fyziologie MeSH
- DNA-polymerasa III chemie fyziologie MeSH
- HeLa buňky MeSH
- homologní rekombinace * MeSH
- jednovláknová DNA biosyntéza MeSH
- lidé MeSH
- osmolární koncentrace MeSH
- poškození DNA MeSH
- proliferační antigen buněčného jádra chemie fyziologie MeSH
- protein FUS vázající RNA chemie fyziologie MeSH
- rekombinasa Rad51 chemie MeSH
- replikace DNA MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
