The protective effect of Enterococcus faecium EFAL41 on chicken's caecum in relation to the TLR (TLR4 and TLR21) activation and production of luminal IgA challenged with Campylobacter jejuni CCM6191 was assessed. The activation of MIF, IFN-β, MD-2 and CD14 was followed-up after bacterial infection. Day-old chicks (40) were divided into four groups (n = 10): control (C), E. faecium AL41 (EFAL41), C. jejuni (CJ) and combined E. faecium AL41+C. jejuni (EFAL41+CJ). Relative mRNA expression of TLR4, TLR21 and CD14 was upregulated in the probiotic strain and infected (combined) group on day 4 and 7 post infection (p.i.). The caecal relative MD-2 mRNA expression was upregulated on day 4 p.i. in the EFAL41+CJ and CJ groups. MIF and IFN-β reached the highest levels in the combined groups on day 7 p.i. The concentration of the sIgA in intestinal flush was upregulated in EFAL41+CJ group on day 4 p.i. The results demonstrated that E. faecium EFAL41 probiotic strain can modulate the TLRs expression and modify the activation of MIF, IFN-β, MD-2 and CD14 molecules in the chickens caecum challenged with C. jejuni CCM 6191. The counts of EFAL41 were sufficient and high, similarly the counts of enterococci in both, caecum and faeces but without reduction of Campylobacter counts.
- MeSH
- Campylobacter jejuni růst a vývoj MeSH
- cékum imunologie mikrobiologie MeSH
- Enterococcus faecium růst a vývoj imunologie MeSH
- feces mikrobiologie MeSH
- imunoglobulin A sekreční genetika MeSH
- interakce hostitele a patogenu MeSH
- interferon beta genetika imunologie MeSH
- kampylobakterové infekce dietoterapie imunologie mikrobiologie veterinární MeSH
- kur domácí MeSH
- lipopolysacharidové receptory genetika imunologie MeSH
- lymfocytární antigen 96 genetika imunologie MeSH
- nemoci drůbeže dietoterapie genetika imunologie mikrobiologie MeSH
- novorozená zvířata MeSH
- probiotika farmakologie MeSH
- protein - isoformy genetika imunologie MeSH
- receptory imunologické genetika imunologie MeSH
- regulace genové exprese MeSH
- signální transdukce MeSH
- toll-like receptor 4 genetika imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
This study presents the effects of the probiotic and enterocin M-producing strain Enterococcus faecium AL41 on microbiota, phagocytic activity (PA), oxidative stress, performance and biochemical parameters when applied individually or in combination with Eleutherococcus senticosus in rabbits. The novelty of the study lies in the use of our non-rabbit-derived strain (AL41 = CCM8558) which produces new enterocin M. Ninety-six post-weaned rabbits (Hyplus breed) aged 5 weeks were divided into three experimental groups, 24 in each: E. senticosus (ES, 30 g/100 kg) in feed, E. faecium AL41 (10(9) CFU/mL marked by rifampicin to differentiate it from other enterococci) in water, and ES + AL. AL41 colonized sufficiently in rabbits to reduce coliforms, staphylococci, pseudomonads and clostridia. Slight decrease in bacteria was also found in the caecum and appendix. Phagocytic activity was significantly increased in the experimental groups compared to the control group (CG) (p < 0.001; p < 0.05). Applications did not evoke oxidative stress. Biochemical parameters in blood and caecal organic acids were slightly influenced. Average daily weight gain was slightly higher in ES and AL + ES. Combinative application of E. faecium with E. senticosus can be beneficial in rabbits. AL41 strain alone and in combination with ES produced reduction in spoilage bacteria; the highest stimulation of PA was in the AL41 + ES group.
- MeSH
- eleuterokok růst a vývoj metabolismus MeSH
- Enterococcus faecium růst a vývoj metabolismus MeSH
- feces mikrobiologie MeSH
- králíci metabolismus mikrobiologie MeSH
- přemostěné cyklické sloučeniny metabolismus MeSH
- probiotika metabolismus MeSH
- střevní mikroflóra účinky léků MeSH
- zvířata MeSH
- Check Tag
- králíci metabolismus mikrobiologie MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
New quaternary ammonium salt-type compounds with lipophilic cholesterol and terpene moieties were synthesized. The compounds showed promising antibacterial and antimycobacterial activities. Those compounds containing the cholesterol moiety showed significant activity against Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecium. On the contrary, the antimycobacterial activity increased with the presence of the terpene unit in the molecule.
- MeSH
- antiinfekční látky chemická syntéza farmakologie MeSH
- cholesterol analogy a deriváty chemická syntéza farmakologie MeSH
- Enterococcus faecium účinky léků růst a vývoj MeSH
- houby účinky léků růst a vývoj MeSH
- kvartérní amoniové sloučeniny chemická syntéza farmakologie MeSH
- mikrobiální testy citlivosti MeSH
- molekulární struktura MeSH
- Mycobacterium účinky léků růst a vývoj MeSH
- racionální návrh léčiv MeSH
- Staphylococcus aureus účinky léků růst a vývoj MeSH
- Staphylococcus epidermidis účinky léků růst a vývoj MeSH
- terpeny diagnostické užití farmakologie MeSH
- vztahy mezi strukturou a aktivitou MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
