Trypanosoma brucei is an important human pathogen. In this study, we have focused on the characterization of FtsH protease, ATP-dependent membrane-bound mitochondrial enzyme important for regulation of protein abundance. We have determined localization and orientation of all six putative T.brucei FtsH homologs in the inner mitochondrial membrane by in silico analyses, by immunofluorescence, and with protease assay. The evolutionary origin of these homologs has been tested by comparative phylogenetic analysis. Surprisingly, some kinetoplastid FtsH proteins display inverted orientation in the mitochondrial membrane compared to related proteins of other examined eukaryotes. Moreover, our data strongly suggest that during evolution the orientation of FtsH protease in T. brucei varied due to both loss and acquisition of the transmembrane domain.

• MeSH
• Arabidopsis klasifikace   enzymologie   genetika   MeSH
• Euglena gracilis klasifikace   enzymologie   genetika   MeSH
• Euglena longa klasifikace   enzymologie   genetika   MeSH
• exprese genu MeSH
• fylogeneze MeSH
• izoenzymy chemie   genetika   metabolismus   MeSH
• konzervovaná sekvence MeSH
• Leishmania major klasifikace   enzymologie   genetika   MeSH
• lidé MeSH
• mitochondriální membrány chemie   enzymologie   MeSH
• mitochondriální proteiny chemie   genetika   metabolismus   MeSH
• mitochondrie enzymologie   genetika   MeSH
• molekulární evoluce * MeSH
• myši MeSH
• proteasy chemie   genetika   metabolismus   MeSH
• proteinové domény MeSH
• protozoální proteiny chemie   genetika   metabolismus   MeSH
• Saccharomyces cerevisiae klasifikace   enzymologie   genetika   MeSH
• Trypanosoma brucei brucei klasifikace   enzymologie   genetika   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Euglenophytes are a familiar algal group with green alga-derived secondary plastids, but the knowledge of euglenophyte plastid function and evolution is still highly incomplete. With this in mind we sequenced and analysed the transcriptome of the non-photosynthetic species Euglena longa. The transcriptomic data confirmed the absence of genes for the photosynthetic machinery, but provided candidate plastid-localised proteins bearing N-terminal bipartite topogenic signals (BTSs) of the characteristic euglenophyte type. Further comparative analyses including transcriptome assemblies available for photosynthetic euglenophytes enabled us to unveil salient aspects of the basic euglenophyte plastid infrastructure, such as plastidial targeting of several proteins as C-terminal translational fusions with other BTS-bearing proteins or replacement of the conventional eubacteria-derived plastidial ribosomal protein L24 by homologs of archaeo-eukaryotic origin. Strikingly, no homologs of any key component of the TOC/TIC system and the plastid division apparatus are discernible in euglenophytes, and the machinery for intraplastidial protein targeting has been simplified by the loss of the cpSRP/cpFtsY system and the SEC2 translocon. Lastly, euglenophytes proved to encode a plastid-targeted homolog of the termination factor Rho horizontally acquired from a Lambdaproteobacteria-related donor. Our study thus further documents a substantial remodelling of the euglenophyte plastid compared to its green algal progenitor.

• MeSH
• Euglena longa klasifikace   cytologie   genetika   MeSH
• fotosyntéza * MeSH
• fylogeneze MeSH
• molekulární evoluce * MeSH
• plastidy genetika   MeSH
• proteiny chloroplastové genetika   MeSH
• sekvence nukleotidů MeSH
• sekvenční homologie MeSH
• stanovení celkové genové exprese MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH