This study aimed to investigate whether heat stress (HS) prevents a decrease in succinate dehydrogenase (SDH) activity and heat shock protein 60 (HSP60) and superoxide dismutase 2 (SOD2) contents in the extensor digitorum longus of streptozotocin (STZ)-induced diabetic rats. Twelve-week-old male Wistar rats were assigned to one of the four groups (n=6/group): control (Con), HS, diabetes mellitus (DM), and diabetes mellitus and heat stress (DM+HS). Diabetes was induced by the administration of STZ (50 mg/kg). HS was initiated 7 days after STZ treatment and performed at 42 °C for 30 min 5 times a week for 3 weeks. SDH activity was decreased in the DM and DM+HS groups. However, SDH activity was greater in the DM+HS group than in the DM group. Although HSP60 content was lower in the DM group than in the Con group, it was maintained in the DM+HS groups and was higher than that in the DM group. SOD2 content was decreased only in the DM group. These findings suggest that HS prevents the decrease in SDH activity in the skeletal muscle induced by DM. According to this mechanism, the maintenance of SOD2 and HSP60 by HS may suppress the increase in oxidative stress.
- MeSH
- aktivace enzymů fyziologie MeSH
- chaperon hsp60 metabolismus MeSH
- experimentální diabetes mellitus metabolismus MeSH
- kosterní svaly enzymologie metabolismus MeSH
- krevní glukóza metabolismus MeSH
- krysa rodu rattus MeSH
- mitochondriální proteiny metabolismus MeSH
- potkani Wistar MeSH
- reakce na tepelný šok fyziologie MeSH
- streptozocin MeSH
- sukcinátdehydrogenasa metabolismus MeSH
- superoxiddismutasa metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Cytokinins (CKs) are phytohormones regulating plant growth and development as well as response to the environment. In order to evaluate their function in heat stress (HS) responses, the effect of CK elevation was determined during three types of HS - targeted to shoots, targeted to roots and applied to the whole plant. The early (30min) and longer term (3h) responses were followed at the hormonal, transcriptomic and proteomic levels in Arabidopsis transformants with dexamethasone-inducible expression of the CK biosynthetic gene isopentenyltransferase (ipt) and the corresponding wild-type (Col-0). Combination of hormonal and phenotypic analyses showed transient up-regulation of the CK/abscisic acid ratio, which controls stomatal aperture, to be more pronounced in the transformant. HS responses of the root proteome and Rubisco-immunodepleted leaf proteome were followed using 2-D gel electrophoresis and MALDI-TOF/TOF. More than 100 HS-responsive proteins were detected, most of them being modulated by CK increase. Proteome and transcriptome analyses demonstrated that CKs have longer term positive effects on the stress-related proteins and transcripts, as well as on the photosynthesis-related ones. Transient accumulation of CKs and stimulation of their signal transduction in tissue(s) not exposed to HS indicate that they are involved in plant stress responses.
- MeSH
- alkyltransferasy a aryltransferasy fyziologie MeSH
- Arabidopsis účinky léků metabolismus fyziologie MeSH
- cytokininy fyziologie MeSH
- dexamethason farmakologie MeSH
- kořeny rostlin metabolismus fyziologie MeSH
- kyselina abscisová fyziologie MeSH
- proteomika MeSH
- reakce na tepelný šok fyziologie MeSH
- regulace genové exprese u rostlin fyziologie MeSH
- regulátory růstu rostlin fyziologie MeSH
- signální transdukce účinky léků fyziologie MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice MeSH
- stanovení celkové genové exprese MeSH
- výhonky rostlin metabolismus fyziologie MeSH
- vysoká teplota MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
In the current study, singlet oxygen formation by lipid peroxidation induced by heat stress (40 °C) was studied in vivo in unicellular green alga Chlamydomonas reinhardtii. Primary and secondary oxidation products of lipid peroxidation, hydroperoxide and malondialdehyde, were generated under heat stress as detected using swallow-tailed perylene derivative fluorescence monitored by confocal laser scanning microscopy and high performance liquid chromatography, respectively. Lipid peroxidation was initiated by enzymatic reaction as inhibition of lipoxygenase by catechol and caffeic acid prevented hydroperoxide formation. Ultra-weak photon emission showed formation of electronically excited species such as triplet excited carbonyl, which, upon transfer of excitation energy, leads to the formation of either singlet excited chlorophyll or singlet oxygen. Alternatively, singlet oxygen is formed by direct decomposition of hydroperoxide via Russell mechanisms. Formation of singlet oxygen was evidenced by the nitroxyl radical 2,2,6,6-tetramethylpiperidine-1-oxyl detected by electron paramagnetic resonance spin-trapping spectroscopy and the imaging of green fluorescence of singlet oxygen sensor green detected by confocal laser scanning microscopy. Suppression of singlet oxygen formation by lipoxygenase inhibitors indicates that singlet oxygen may be formed via enzymatic lipid peroxidation initiated by lipoxygenase.
- MeSH
- Chlamydomonas reinhardtii metabolismus MeSH
- lipoxygenasa metabolismus MeSH
- malondialdehyd metabolismus MeSH
- peroxidace lipidů fyziologie MeSH
- reakce na tepelný šok fyziologie MeSH
- rostlinné proteiny metabolismus MeSH
- singletový kyslík metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
In this study, lipoic acid and heat shock treatments were applied to C(2)C(12) myotubes and Sprague-Dawley rats to investigate changes in the heat shock protein 70 (HSP70) and glucose transporter 4 (GLUT4) in 4 different skeletal muscle groups. The results of western blotting indicated that treatment of lipoic acid for 24 h, heat-shock and combined lipoic acid and heat-shock which all increased the level of HSP70 substantially in C(2)C(12) myotubes. However, either lipoic acid or heat-shock did not increase the level of GLUT4 in C(2)C(12) myotubes. In an in vitro migration assay, lipoic acid increased wound migration only when it was applied for 3 h. Moreover, our in vivo results revealed that lipoic acid did not increase HSP70 and GLUT4 in all 4 different skeletal muscles. Furthermore, heat-shock increased HSP70 in all 4 different muscle groups, and heat-shock treatment alone increased the GLUT4 in the soleus muscle only, suggesting that the GLUT4 increased by heat-shock was slow-twitch muscle specific. Collectively, our results indicated that heat-shock is critical factor that modulates GLUT4 and HSP70 in the skeletal muscle of rats.
- MeSH
- buněčné linie MeSH
- kosterní svaly fyziologie MeSH
- krysa rodu rattus MeSH
- potkani Sprague-Dawley MeSH
- přenašeč glukosy typ 4 metabolismus MeSH
- proteiny tepelného šoku HSP70 metabolismus MeSH
- reakce na tepelný šok fyziologie MeSH
- svalová vlákna typu I fyziologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- cytoplazmatická granula * genetika metabolismus MeSH
- fyziologie buňky fyziologie genetika MeSH
- posttranslační úpravy proteinů MeSH
- reakce na tepelný šok * fyziologie genetika MeSH
- ribonukleoproteiny genetika metabolismus MeSH
- Saccharomyces cerevisiae * fyziologie genetika metabolismus MeSH
- signální dráha UPR MeSH
- MeSH
- homeostáza fyziologie genetika MeSH
- lidé MeSH
- mitochondriální DNA genetika metabolismus MeSH
- oxidační stres fyziologie genetika MeSH
- proteiny teplotního šoku metabolismus MeSH
- proteiny vázající telomery genetika metabolismus MeSH
- reakce na tepelný šok fyziologie genetika MeSH
- reaktivní formy kyslíku metabolismus MeSH
- stárnutí buněk fyziologie genetika MeSH
- stárnutí fyziologie genetika MeSH
- život MeSH
- Check Tag
- lidé MeSH
- MeSH
- chinony antagonisté a inhibitory farmakokinetika imunologie MeSH
- estradiol farmakokinetika imunologie MeSH
- eukaryotické buňky cytologie účinky léků MeSH
- finanční podpora výzkumu jako téma MeSH
- kvasinky účinky léků MeSH
- prednisolon antagonisté a inhibitory farmakokinetika imunologie MeSH
- proteiny tepelného šoku HSP90 analýza farmakokinetika MeSH
- reakce na tepelný šok fyziologie imunologie účinky léků MeSH
- rifabutin antagonisté a inhibitory farmakokinetika imunologie MeSH
- Publikační typ
- srovnávací studie MeSH
The heat shock response, characterized by increased expression of heat shock proteins (Hsps) is induced by exposure of cells and tissues to extreme conditions that cause acute or chronic stress. Hsps function as molecular chaperones in regulating cellular homeostasis and promoting survival. If the stress is too severe, a signal that leads to programmed cell death, apoptosis, is activated, thereby providing a finely tuned balance between survival and death. In addition to extracellular stimuli, several nonstressfull conditions induce Hsps during normal cellular growth and development. The enhanced heat shock gene expression in response to various stimuli is regulated by heat shock transcription factors.
- MeSH
- finanční podpora výzkumu jako téma MeSH
- fyziologická adaptace MeSH
- hypotenze MeSH
- krevní tlak genetika MeSH
- krysa rodu rattus MeSH
- oxid dusnatý analýza antagonisté a inhibitory fyziologie MeSH
- proteiny tepelného šoku HSP70 antagonisté a inhibitory fyziologie MeSH
- reakce na tepelný šok fyziologie MeSH
- synthasa oxidu dusnatého analýza antagonisté a inhibitory biosyntéza MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
