Oleaginous microbes, which contain over 20% intracellular lipid, predominantly triacylglycerols (TG), by dry weight, have been discovered to have high oil content by many different protocols, ranging from simple staining to more complex chromatographic methods. In our laboratory, a methodical process was implemented to identify high oil yeasts, designed to minimize labor while optimizing success in identifying high oil strains among thousands of candidates. First, criteria were developed to select candidate yeast strains for analysis. These included observation of buoyancy of the yeast cell mass in 20% glycerol, and phylogenetic placement near known oleaginous species. A low-labor, semiquantitative Nile red staining protocol was implemented to screen numerous yeast cultures for high oil content in 96-well plates. Then, promising candidates were selected for more quantitative analysis. A more labor-intensive and quantitative gravimetric assay was implemented that gave consistent values for intracellular oil content for a broad range of yeast species. Finally, an LC-MS protocol was utilized to quantify and identify yeast triacylglycerols. This progressive approach was successful in identifying 30 new oleaginous yeast species, out of over 1000 species represented in the Phaff Yeast Culture Collection.
- MeSH
- barvení a značení metody MeSH
- chromatografie kapalinová metody MeSH
- fluorescenční barviva analýza MeSH
- fluorescenční mikroskopie metody MeSH
- hmotnostní spektrometrie metody MeSH
- kvasinky chemie MeSH
- lipidomika metody MeSH
- lipidy analýza MeSH
- oxaziny analýza MeSH
- triglyceridy analýza MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH
The increasing use of neonicotinoids in systematic seed treatment to crops is a serious cause of pollution of water resources and environment. Consequently, food sources can get eventually contaminated. To this end, it is desirable to develop suitable and effective platforms in order to obtain low-cost and sensitive sensors for neonicotinoids detection. In this work, graphene oxide modified electrodes were used as highly efficient electrochemical sensors for detection of two common insecticides - thiamethoxam and imidacloprid. The proposed sensor responded linearly in the concentration range of 10-200µmolL(-1) for both analytes and the detection limits were determined as low as 8.3µmolL(-1) and 7.9µmolL(-1) for thiamethoxam and imidacloprid, respectively. Analytical performance was also evaluated on spiked water and honey samples.
- MeSH
- analýza potravin ekonomika metody MeSH
- biosenzitivní techniky ekonomika metody MeSH
- chemické látky znečišťující vodu analýza MeSH
- dusíkaté sloučeniny analýza MeSH
- elektrochemické techniky ekonomika metody MeSH
- elektrody MeSH
- grafit chemie MeSH
- imidazoly analýza MeSH
- insekticidy analýza MeSH
- kontaminace potravin analýza MeSH
- limita detekce MeSH
- med analýza MeSH
- monitorování životního prostředí ekonomika metody MeSH
- oxaziny analýza MeSH
- oxidy chemie MeSH
- řeky chemie MeSH
- thiazoly analýza MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
