We provide the complete sequence of a virus tentatively named "Tetranychus urticae-associated picorna-like virus 1PK13" (TuaPV1-PK13) obtained from the high-throughput sequencing of a symptomless apple leaf sample. Although the virus sequence was originally derived from apple leaves, the data suggest that the virus is associated with the two-spotted mite Tetranychus urticae.
Population of wild boar is increasing in the whole Europe, the animals migrate close to human habitats which greatly increases the possibility of natural transmission between domestic animals or humans and wild boars. The aim of the study was to estimate in population of free-living wild boar in the Czech Republic the prevalence of enteric viral pathogens, namely rotavirus groups A and C (RVA and RVC), porcine reproductive and respiratory syndrome virus (PRRSV), and members of family Coronaviridae (transmissible gastroenteritis virus - TGEV, porcine epidemic diarrhea virus - PEDV, porcine respiratory coronavirus - PRCV, and porcine hemagglutination encephalomyelitis virus - PHEV) and Picornaviridae,(teschovirus A - PTV, sapelovirus A - PSV, and enterovirus G - EV-G). In our study, stool samples from 203 wild boars culled during hunting season 2014-2015 (from October to January) were examined by RT-PCR. RVA was detected in 2.5% of tested samples. Nucleotide analysis of VP7, VP4, and VP6 genes revealed that four RVA strains belong to G4P[25]I1, G4P[6]I5, G11P[13]I5, and G5P[13]I5 genotypes and phylogenetic analysis suggested close relation to porcine and human RVAs. The prevalence of RVC in wild boar population reached 12.8%, PTV was detected in 20.2%, PSV in 8.9%, and EV-G in 2.5% of samples. During our study no PRRSV or coronaviruses were detected. Our study provides the first evidence of RVC prevalence in wild boars and indicates that wild boars might contribute to the genetic variability of RVA and also serve as an important reservoir of other enteric viruses.
- MeSH
- antigeny virové genetika MeSH
- Coronaviridae genetika izolace a purifikace MeSH
- feces virologie MeSH
- fylogeneze MeSH
- genotyp MeSH
- infekce viry z čeledi Coronaviridae epidemiologie veterinární virologie MeSH
- lidé MeSH
- nemoci prasat epidemiologie virologie MeSH
- Picornaviridae genetika izolace a purifikace MeSH
- pikornavirové infekce epidemiologie veterinární virologie MeSH
- prasata MeSH
- rotavirové infekce epidemiologie veterinární virologie MeSH
- Rotavirus genetika izolace a purifikace MeSH
- Sus scrofa MeSH
- virové plášťové proteiny genetika MeSH
- zdroje nemoci MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
Původci virových gastroenteritid patří mezi obecně méně známou skupinu patogenních mikroorganizmů. Podle studií však tvoří nemalou část kauzálních agens průjmových onemocnění dětí i dospělých. V této rešeršní práci se autoři zaměřili jak na obecně relativně dobře známé zástupce této skupiny, jako adenoviry, rotaviry a kaliciviry, tak i na původce méně známé či nové, jako toroviry, astroviry, bocaviry či parechoviry. V první části článku se pojednává v základních informacích o jednotlivých etiologických činitelích, v druhé pak o jejich detekci a identifikaci. V poslední části je předložena krátká informace o terapii a možné profylaxi.
The causative agents of viral gastroenteritis belong to generally little known pathogens. But according to some studies, these viruses make up a considerable proportion of agents causing diarrhea in children and adults. In this review, the authors focused on both relatively known pathogens such as adenoviruses, rotaviruses or caliciviruses and not so well known or newly recognized viral gastroenteritis pathogens like toroviruses, astroviruses, bocaviruses or parechoviruses. The first part of this review is concerned with basic information about each etiological agent. The second one deals with their detection and identification. The last part is about therapy and prophylaxis of viral pathogens.
- Klíčová slova
- původci, diagnostika,
- MeSH
- Adenoviridae izolace a purifikace patogenita MeSH
- Astroviridae izolace a purifikace patogenita MeSH
- Caliciviridae izolace a purifikace patogenita MeSH
- feces virologie MeSH
- financování organizované MeSH
- gastroenteritida diagnóza etiologie terapie MeSH
- lidé MeSH
- Parvoviridae izolace a purifikace patogenita MeSH
- Picornaviridae izolace a purifikace patogenita MeSH
- polymerázová řetězová reakce metody MeSH
- Rotavirus izolace a purifikace patogenita MeSH
- virologie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
This study presents results of epidemiological survey and genetic characterisation of porcine enteric picornaviruses belonging to the genera Teschovirus, Sapelovirus, and Porcine enterovirus B. Faecal or gut content samples from domestic pigs (Sus scrofa f. domestica) and the cecal content of wild boars (Sus scrofa) of different ages (collected between 2005 and 2011) were analysed by molecular methods. Porcine enterovirus B was the most prevalent virus detected in both domestic pigs and wild boars (50.2% and 69.4%, respectively), followed by Porcine teschovirus and Porcine sapelovirus. The majority of positive domestic pigs (69.4%) and wild boars (64.3%) were infected with two or three tested viruses. There was no significant difference in prevalences of teschoviruses, sapeloviruses, and enteroviruses among healthy and diarrhoeic pigs. Results of epidemiological survey demonstrated that all target viral genera are common in Czech farms producing pigs and wild boars. Amplified nucleotide fragments of VP2 region obtained from randomly selected both historical and recent Teschovirus isolates were sequenced. Based on sequence data, historical Porcine teschovirus isolate CAPM V-180, previously determined as serotype 1 was reclassified into serotype 11. Moreover, another recent Porcine teschovirus isolate OH264/2010 was described and classified into serotype 11. Four nontypeable PTV strains (historical isolate CAPM V-182/1976 and recent isolates JA247/2010, NI429/2010, and BR1576/2007) identified in this study might represent novel serotypes. To the best of our knowledge, our study represents the first description of this serotype in the Czech Republic.
- MeSH
- cékum virologie MeSH
- enteroviry prasat klasifikace genetika izolace a purifikace MeSH
- feces virologie MeSH
- fylogeneze MeSH
- molekulární typizace MeSH
- Picornaviridae klasifikace genetika izolace a purifikace MeSH
- pikornavirové infekce epidemiologie veterinární virologie MeSH
- prasata virologie MeSH
- prevalence MeSH
- sérotypizace MeSH
- surveillance populace MeSH
- Sus scrofa virologie MeSH
- Teschovirus klasifikace genetika izolace a purifikace MeSH
- virové plášťové proteiny genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH