Control of common scab disease can be reached by resistant cultivars or suppressive soils. Both mechanisms are likely to translate into particular potato microbiome profiles, but the relative importance of each is not known. Here, microbiomes of bulk and tuberosphere soil and of potato periderm were studied in one resistant and one susceptible cultivar grown in a conducive and a suppressive field. Disease severity was suppressed similarly by both means yet, the copy numbers of txtB gene (coding for a pathogenicity determinant) were similar in both soils but higher in periderms of the susceptible cultivar from conducive soil. Illumina sequencing of 16S rRNA genes for bacteria (completed by 16S rRNA microarray approach) and archaea, and of 18S rRNA genes for micro-eukarytes showed that in bacteria, the more important was the effect of cultivar and diversity decreased from resistant cultivar to bulk soil to susceptible cultivar. The major changes occurred in proportions of Actinobacteria, Chloroflexi, and Proteobacteria. In archaea and micro-eukaryotes, differences were primarily due to the suppressive and conducive soil. The effect of soil suppressiveness × cultivar resistance depended on the microbial community considered, but differed also with respect to soil and plant nutrient contents particularly in N, S and Fe.

• MeSH
• Actinobacteria klasifikace   genetika   růst a vývoj   patogenita   MeSH
• Archaea klasifikace   genetika   růst a vývoj   patogenita   MeSH
• Chloroflexi klasifikace   genetika   růst a vývoj   patogenita   MeSH
• dusík metabolismus   farmakologie   MeSH
• eukaryotické buňky metabolismus   MeSH
• faktory virulence genetika   metabolismus   MeSH
• genotypizační techniky MeSH
• mikrobiota genetika   MeSH
• náchylnost k nemoci imunologie   MeSH
• nemoci rostlin imunologie   mikrobiologie   MeSH
• odolnost vůči nemocem účinky léků   MeSH
• Proteobacteria klasifikace   genetika   růst a vývoj   patogenita   MeSH
• půdní mikrobiologie * MeSH
• RNA ribozomální 16S genetika   MeSH
• RNA ribozomální 18S genetika   MeSH
• síra metabolismus   farmakologie   MeSH
• Solanum tuberosum účinky léků   imunologie   mikrobiologie   MeSH
• železo metabolismus   farmakologie   MeSH
• zemědělské plodiny MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Bacterial clone libraries of the gut microbiota of nurtured and starved Cylindroiulus fulviceps specimens displayed the predominance of the phyla Bacteroidetes (55 and 37 %, respectively) and Proteobacteria (40 and 35 %, respectively) and a high similarity to bacteria previously detected in the intestinal tract of termites and beetles, which are known to harbor symbiotic bacteria essential for digestive activity. Bacterial isolates were dominated by Proteobacteria (74 %), followed by members of the phyla Actinobacteria, Firmicutes and Bacteroidetes. PCR-DGGE fingerprints of the gut samples showed that intestinal bacteria were affected by starvation, although the change was not significant.

• MeSH
• Actinobacteria genetika   izolace a purifikace   klasifikace   MeSH
• Bacteria genetika   izolace a purifikace   klasifikace   MeSH
• Bacteroidetes genetika   izolace a purifikace   klasifikace   MeSH
• bakteriologické techniky MeSH
• členovci mikrobiologie   MeSH
• DNA bakterií genetika   MeSH
• DNA fingerprinting metody   MeSH
• ekosystém MeSH
• elektroforéza v agarovém gelu metody   MeSH
• financování organizované MeSH
• fylogeneze MeSH
• gastrointestinální trakt mikrobiologie   MeSH
• klonování DNA metody   MeSH
• kultivační média MeSH
• metagenom MeSH
• molekulární sekvence - údaje MeSH
• Proteobacteria genetika   izolace a purifikace   klasifikace   MeSH
• RNA ribozomální 16S genetika   MeSH
• sekvenční analýza DNA MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH

As part of the EU project MULTIBARRIERS, six new endogenous aerobic bacterial isolates able to grow in the presence of BTmX (benzene, toluene, m-xylene) were characterized with respect to their growth specificities. Preliminary analysis included restriction fragment length polymorphism profiles and 16S rDNA sequencing. The diversity of these strains was confirmed by denaturing gradient gel electrophoresis. Additional aerobic bacterial strains were isolated from the rhizospheres of plants grown in polychlorinated biphenyl (PCB)-contaminated soils. Pot experiments were designed to show the beneficial effect of plants on the bacterial degradation of PCBs. The effect of PCB removal from soil was evaluated and bacteria isolated from three different plant species were examined for the presence of the bph operon.

• MeSH
• Actinobacteria genetika   klasifikace   metabolismus   MeSH
• aromatické uhlovodíky metabolismus   MeSH
• biodegradace MeSH
• chemické látky znečišťující vodu metabolismus   MeSH
• dekontaminace metody   MeSH
• ekosystém MeSH
• financování organizované MeSH
• kořeny rostlin mikrobiologie   MeSH
• látky znečišťující půdu metabolismus   MeSH
• Proteobacteria genetika   klasifikace   metabolismus   MeSH
• půdní mikrobiologie MeSH
• rostliny mikrobiologie   MeSH

• MeSH
• Bacteria klasifikace   MeSH
• bakteriologie MeSH
• klasifikace metody   MeSH
• Proteobacteria klasifikace   MeSH
• Publikační typ
• přehledy MeSH

• Konspekt
• Mikrobiologie
• NLK Obory
• bakteriologie