Nitric oxide (NO) is identified as a signaling molecule involved in many cellular or physiological functions including meiotic maturation and parthenogenetic activation of mammalian oocytes. We observed that nitric oxide donor SNAP was potent to induce parthenogenetic activation in Xenopus eggs. NO-scavenger CPTIO impaired the effects of SNAP, providing evidence for the effects of the latter to be specific upon NO release. In Xenopus eggs, SNAP treatment induced pigment rearrangement, pronucleus formation and exocytosis of cortical granules. At a biochemical level, SNAP exposure lead to MAPK and Rsk inactivation within 30 minutes whereas MPF remained active, in contrast to calcium ionophore control where MPF activity dropped rapidly. MAPK inactivation could be correlated to pronuclear envelope reformation observed. In SNAP-treated eggs, a strong increase in intracellular calcium level was observed. NO effects were impaired in calcium-free or calcium limited medium, suggesting that that parthenogenetic activation of Xenopus oocytes with a NO donor was mainly calcium-dependent.

• MeSH
• aktivace enzymů účinky léků   MeSH
• aparát dělícího vřeténka účinky léků   metabolismus   MeSH
• benzoáty farmakologie   MeSH
• donory oxidu dusnatého farmakologie   MeSH
• faktor podporující zrání metabolismus   MeSH
• imidazoly farmakologie   MeSH
• kinetika MeSH
• mitogenem aktivované proteinkinasy metabolismus   MeSH
• morfogeneze účinky léků   MeSH
• ovum cytologie   účinky léků   metabolismus   MeSH
• oxid dusnatý metabolismus   MeSH
• partenogeneze MeSH
• progesteron farmakologie   MeSH
• S-nitroso-N-acetylpenicilamin farmakokinetika   MeSH
• vápník metabolismus   MeSH
• Xenopus laevis metabolismus   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH