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14 záznamů v Medvik Filtry

Článek

Riboflavin recovery of spermatogenic dysfunction via a dual inhibition of oxidative changes and regulation of the PINK1-mediated pathway in arsenic-injured rat model

Olfati, A
Autor Olfati, A Young Researchers and Elites Club, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
Tvrdá, Eva
Autor Autorita ORCID Department of Animal Physiology, Slovak University of Agriculture, Nitra, Slovakia

Physiological research. 2021 ; 70 (4) : 591-603. [pub] 20210601

Physiol. Res. (Print)
ISSN 1802-9973
Medvik
Zdroj

Arsenic trioxide (As2O3) poisoning and associated potential lesions are of a global concern. Inversely, riboflavin (vitamin B2, VB2) as a component of flavoproteins could play a vital role in the spermatogenic enzymatic reactions. Thus, this research aimed to explore potential beneficial roles of VB2 during As2O3-injured-toxicity. Rats were randomly allocated into 4 groups (n=8/group) and challenged as follows (for 30 days continuously): Group 1 received normal saline; Group 2 was treated with 3 mg As2O3/L; Group 3 received 40 mg VB2/L; Group 4 received 3 mg As2O3/L + 40 mg VB2/L. Both As2O3 and VB2 were dissolved in deionized water. Malondialdehyde (MDA), Glutathione Peroxidase (GSH-Px), Superoxide dismutase (SOD), and Catalase (CAT) were assessed for the oxidative profile, while TAS (Total Antioxidative Status) levels were evaluated for the antioxidant system, in both serum and testicular tissue. P<0.05 was considered statistically significant. The results show that As2O3 significantly decreased the body weight, testicular weight and testis volume, semen quality and testicular cell count (p<0.05). Furthermore, MDA content in the testicular tissue of the As2O3 group rats was significantly higher in comparison to the vehicle group (p<0.05). Likewise, TAS and the activities of GSH-Px, CAT and SOD were reduced (p<0.05) when compared to the control. As(2)O(3) induced testicular damage and seminiferous tubular atrophy. Monodansylcadaverine assays mirrored the histopathology observations. Meanwhile, As2O3 upregulated the expression of mitophagy-related genes including PINK1, Parkin, USP8, LC3-I, Fis1 and Mfn2. The p38 gene, responsible to stress stimuli, was also upregulated by As2O3 administration. Meanwhile, exposure to VB2 led to a significant decrease of the expression levels of mitophagy related genes. Our study revealed that VB2 supplementation protected testicular structures against As2O3-induced injury via a dual inhibition of oxidative changes and a regulation of the PINK1-mediated pathway.

MeSH
antioxidancia farmakologie MeSH
mitofagie účinky léků MeSH
oxid arsenitý toxicita MeSH
oxidační stres účinky léků MeSH
potkani Wistar MeSH
proteinkinasy genetika metabolismus MeSH
proteiny spojené s autofagií genetika metabolismus MeSH
riboflavin farmakologie MeSH
signální transdukce MeSH
spermatogeneze účinky léků MeSH
spermie účinky léků enzymologie patologie MeSH
testis účinky léků enzymologie patologie MeSH
zvířata MeSH
Check Tag
mužské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH

Článek

Evaluation of hypotesticular activities of different solvent fractions of hydro-methanolic extract of the fruit of Terminalia chebula in Wistar strain adult albino rat: Genomic and flow cytometric approaches

Journal of Applied Biomedicine. 2018 ; 16 (4) : 394-400.

ISSN 1214-021X
Medvik
Zdroj

Fruit of Terminalia chebula Retz. (Combretaceae) has male contraceptive folk medicine reputation but its molecular aspect regarding hypotesticular activity is still in dark. The study focused the hypotesticular efficacy of the most potent fraction out of n-hexane, chloroform and ethyl acetate fractions of hydro-methanolic (3:2) extract of Terminalia chebula in connection with male herbal contraceptive development. Treatment with above fractions of Terminalia chebula showed a significant diminution in the activities of androgenic key enzymes (Δ5, 3β-HSD, 17β-HSD) and inhibition in serum testosterone level in compare to the control. Significant up regulation of testicular Bax gene and down regulation of Bcl-2 gene indicated the hypotesticular activity of these fractions. Flow-cytometric study focused a significant diminution in sperm viability and sperm mitochondrial status after the treatment with different fractions. Out of these, ethyl acetate fraction showed most promising hypotesticular effect without impairing any toxicity in general which highlighted that the fraction may contains antitesticular agent(s) in threshold levels compare to other fractions as it decreases spermiological, testicular genomic sensors and elevates sperm apoptotic sensors that may lead to male contraception.

MeSH
látky blokující spermatogenezi * chemie izolace a purifikace MeSH
modely u zvířat MeSH
potkani Wistar MeSH
průtoková cytometrie MeSH
rostlinné extrakty MeSH
spermie účinky léků MeSH
Terminalia chemie MeSH
testis enzymologie chemie účinky léků MeSH
zvířata MeSH
Check Tag
mužské pohlaví MeSH
zvířata MeSH
Publikační typ
práce podpořená grantem MeSH

Článek

Endocrine disruptors and other inhibitors of 11β-hydroxysteroid dehydrogenase 1 and 2: Tissue-specific consequences of enzyme inhibition

Journal of steroid biochemistry and molecular biology. 2016 ; 155 (Pt B) : 207-216. [pub] 20140724

J Steroid Biochem Mol Biol
ISSN 1879-1220
Medvik
Zdroj

Numerous chemicals in the environment have the ability to interact with the endocrine system. These compounds are called endocrine disruptors (EDs). Exposure to EDs represents one of the hypotheses for decreasing fertility, the increased risk of numerous cancers and obesity, metabolic syndrome and type 2 diabetes. There are various mechanisms of ED action, one of which is their interference in the action of 11β-hydroxysteroid dehydrogenase (11βHSD) that maintains a balance between active and inactive glucocorticoids on the intracellular level. This enzyme has two isoforms and is expressed in various tissues. Inhibition of 11βHSD in various tissues can have different consequences. In the case of EDs, the results of exposure are mainly adverse; on the other hand pharmaceutically developed inhibitors of 11βHSD type 1 are evaluated as an option for treating metabolic syndrome, as well as related diseases and depressive disorders. This review focuses on the effects of 11βHSD inhibitors in the testis, colon, adipose tissue, kidney, brain and placenta.

Článek

Molecular and biochemical characterisation of human short-chain dehydrogenase/reductase member 3 (DHRS3)

Chemico-biological interactions. 2015 ; 234 (-) : 178-87.

Chem Biol Interact
ISSN 1872-7786
Medvik
Zdroj

Dehydrogenase/reductase (SDR family) member 3 (DHRS3), also known as retinal short-chain dehydrogenase/reductase (retSDR1) is a member of SDR16C family. This family is thought to be NADP(H) dependent and to have multiple substrates; however, to date, only all-trans-retinal has been identified as a DHRS3 substrate. The reductive reaction catalysed by DHRS3 seems to be physiological, and recent studies proved the importance of DHRS3 for maintaining suitable retinoic acid levels during embryonic development in vivo. Although it seems that DHRS3 is an important protein, knowledge of the protein and its properties is quite limited, with the majority of information being more than 15 years old. This study aimed to generate a more comprehensive characterisation of the DHRS3 protein. Recombinant enzyme was prepared and demonstrated to be a microsomal, integral-membrane protein with the C-terminus oriented towards the cytosol, consistent with its preference of NADPH as a cofactor. It was determined that DHRS3 also participates in the metabolism of other endogenous compounds, such as androstenedione, estrone, and DL-glyceraldehyde, and in the biotransformation of xenobiotics (e.g., NNK and acetohexamide) in addition to all-trans-retinal. Purified and reconstituted enzyme was prepared for the first time and will be used for further studies. Expression of DHRS3 was shown at the level of both mRNA and protein in the human liver, testis and small intestine. This new information could open other areas of DHRS3 protein research.

MeSH
alkoholoxidoreduktasy metabolismus MeSH
cytosol metabolismus MeSH
jaterní mikrozomy enzymologie metabolismus MeSH
játra enzymologie metabolismus MeSH
lidé MeSH
membránové proteiny metabolismus MeSH
NADH, NADPH oxidoreduktasy metabolismus MeSH
NADP metabolismus MeSH
Sf9 buňky MeSH
Spodoptera metabolismus MeSH
syntázy mastných kyselin metabolismus MeSH
tenké střevo enzymologie metabolismus MeSH
testis enzymologie metabolismus MeSH
tretinoin metabolismus MeSH
zvířata MeSH
Check Tag
lidé MeSH
mužské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH

Článek

Naringenin attenuates testicular damage, germ cell death and oxidative stress in streptozotocin induced diabetic rats: naringenin prevents diabetic rat testicular damage

Journal of Applied Biomedicine. 2013 ; 11 (3) : 195-208.

J. Appl. Biomed. (Čes. Budějovice Print)
ISSN 1214-021X
Medvik
Zdroj

The aim of this study was to investigate the protective effect of naringenin on oxidative stress, on proinflammatory cytokines like TGF-beta1, IL-1beta and on programmed cell death in the testicular damage resulting from streptozotocin (STZ) induced diabetes in rats. Diabetes was induced by a single intraperitoneal injection of STZ (50 mg/kg), and the rats were treated with naringenin (5 mg/kg and 10 mg/kg) administered once a day orally for 10 weeks, starting 3 days after the STZ injection. At the end of the study, all animals were sacrificed. Testis tissue and blood samples were collected for the assessment of sperm parameters, and for biochemical and histopathological analysis. Naringenin treatment significantly decreased the levels of elevated tissue TBARS (thio-barbituric acid) and increased the superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) enzyme activities in the testis tissues. The naringenin-treated rats in the diabetic group showed an improved histological appearance, sperm parameters, and serum testosterone levels, along with a decrement of terminal dUTP nick end-labeling (TUNEL) detected program cell death and a reduced over expression of TGF-beta1, IL-1beta in Sertoli cells and Leydig cells. These results suggest that naringenin is a food supplement potentially beneficial in reducing testicular damage in diabetic rats by decreasing the oxidative stress related to programmed cell death.

Abstrakt

To hlavní o antimülleriánském hormonu

Hampl, Richard, 1941-
Autor Autorita Endokrinologický ústav, Praha

Diabetologie .... 2013 ; () : 126-131.

ISBN 978-80-7387-656-2
Medvik
Zdroj

Článek

A mouse speciation gene encodes a meiotic histone H3 methyltransferase

Science. 2009 ; 323 (5912) : 373-375.

Medvik
Zdroj

Speciation genes restrict gene flow between the incipient species and related taxa. Three decades ago, we mapped a mammalian speciation gene, hybrid sterility 1 (Hst1), in the intersubspecific hybrids of house mouse. Here, we identify this gene as Prdm9, encoding a histone H3 lysine 4 trimethyltransferase. We rescued infertility in male hybrids with bacterial artificial chromosomes carrying Prdm9 from a strain with the "fertility" Hst1(f) allele. Sterile hybrids display down-regulated microrchidia 2B (Morc2b) and fail to compartmentalize gammaH2AX into the pachynema sex (XY) body. These defects, seen also in Prdm9-null mutants, are rescued by the Prdm9 transgene. Identification of a vertebrate hybrid sterility gene reveals a role for epigenetics in speciation and opens a window to a hybrid sterility gene network.

Článek

Tissue expression and enzymologic characterization of human prostate specific membrane antigen and its rat and pig orthologs

The Prostate. 2008 ; 68 (2) : 171-182.

Prostate
ISSN 0270-4137
Medvik
Zdroj

BACKGROUND: Prostate specific membrane antigen (PSMA), also called glutamate carboxypeptidase II (GCPII), is a target enzyme for diagnosis and treatment of prostate cancer. Moreover, it is upregulated in the vasculature of most solid tumors and is therefore a potential target for the generation of novel antineoplastics. In this context, we analyze the possibility of using rat and pig as animal models for enzymologic and in vivo studies. METHODS: We prepared the recombinant extracellular part of human, rat, and pig GCPII in S2 cell media and characterized the activity and inhibition profiles of the three orthologs by radioenzymatic assay. We performed Western blot analysis of GCPII expression in human, rat, and pig tissues using the monoclonal antibody GCP-04 and confirmed these findings by activity measurements and immunohistochemistry. RESULTS: The three recombinant proteins show similar specific enzymatic activities and inhibition profiles. Tissue expression analysis revealed that most of the pig and human tissues show at least some GCPII-positivity, while the expression pattern in rat is more restricted. Moreover, tissues such as prostate and testes exhibit different GCPII expression levels among the species studied. CONCLUSIONS: The rat and pig orthologs of GCPII seem to be suitable to approximate human GCPII in enzymologic studies. However, the diffuse expression pattern of GCPII in animal and human tissues could be a caveat for the potential utilization of GCPII-targeted anticancer drugs. Furthermore, variations in GCPII tissue distribution among the species studied should be considered when using rat or pig as models for antineoplastic drug discovery.