Vibrio cholerae (Vc) has been isolated from roots of aquatic plants during epidemic or interepidemic periods. It has been suggested that the lectins from the roots of aquatic plants play a role as reservoirs of Vc. In this paper, we evaluated the activity of lectins from Lemna minor and Eichornia crassipens plants as potential mediators of the Vc strain El Tor (Vct). We found that Lemna minor extract showed high specificity towards blood groups O and B. Eichornia crassipens extract showed high specificity towards blood group A and O. Sugar competition experiments demonstrated that Lemna minor extract showed a high recognition to Neu5Ac (acid N acetyl neuraminic or sialic acid) and GlcNAc (N-acetyl D glucoseamine) in group B; and GlcNAc in group O. Eichornia crassipens, the recognition was that of GalNAc (N-acetyl-D-galactoseamine) and GlcNAc in group O; and Fuc (L-Fucose) and GlcNAc in group A. Lemna minor and Eichornia crassipens protein extracts (p-ext) increased Vct proliferation and protected to the red cells group O against the hemolytic activity of Vct. Both p-exts did not show any statistical significance on agglutination to Vct when compared to the results from phosphate buffer. According to the results, lectins present in roots may be involved in the proliferation and survival of Vct.
- Klíčová slova
- Lemna minor,
- MeSH
- Araceae chemie MeSH
- lektiny * analýza MeSH
- lidé MeSH
- Pontederiaceae chemie MeSH
- Vibrio cholerae * izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- MeSH
- bakteriologické techniky klasifikace MeSH
- Enterococcus izolace a purifikace klasifikace MeSH
- Haemophilus influenzae izolace a purifikace klasifikace účinky léků MeSH
- mikrobiální testy citlivosti statistika a číselné údaje MeSH
- řízení kvality MeSH
- Staphylococcus lugdunensis izolace a purifikace klasifikace MeSH
- Streptococcus pyogenes izolace a purifikace MeSH
- testování odbornosti laboratoří * metody normy statistika a číselné údaje MeSH
- Vibrio cholerae izolace a purifikace klasifikace MeSH
- Publikační typ
- tabulky MeSH
- Geografické názvy
- Česká republika MeSH
The occurrence of Vibrio cholerae, an important aquatic pathogen, was assessed in the surface water of the Danube River near Bratislava. The isolates were distinguished by biochemical tests and grouped by ARDRA to three clusters corresponding to three species (V. cholerae, Vibrio metschnikovii, and Aeromonas spp.). The identification of V. cholerae was confirmed by multiplex PCR using primer pairs targeted to ompW gene (membrane protein), ctxA gene (toxicity gene), and toxR gene (regulatory gene). None from the isolated V. cholerae from surface water contained ctxA gene; seven of them possessed toxR gene. Serotyping of V. cholerae isolates with polyvalent O antiserum and O/139 antiserum was negative. All isolates of V. cholerae were susceptible to chloramphenicol, rifampicin, tetracycline, variable to ampicillin, and resistant to kanamycin and streptomycin.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální proteiny genetika MeSH
- cholerový toxin genetika MeSH
- DNA bakterií genetika MeSH
- DNA primery genetika MeSH
- mikrobiální testy citlivosti MeSH
- molekulární typizace MeSH
- multiplexová polymerázová řetězová reakce metody MeSH
- řeky MeSH
- sérotypizace MeSH
- sladká voda mikrobiologie MeSH
- techniky typizace bakterií MeSH
- Vibrio cholerae klasifikace genetika izolace a purifikace metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Slovenská republika MeSH
All the V. cholerae non-O1, non-O139 isolates from ground and surface water samples collected during the rainy season (rainfall contributes significantly in the spread of cholera) contained ompW and a regulatory toxR gene, while many others possessed accessory cholera toxin (ace), hemolysin (hlyA) and outer membrane protein (ompU) genes. All the isolates lacked ctxAB, tcp, zot, rfbO1 and rfbO139 genes. The strains could be grouped into two main clusters colligating the isolates from ground water and surface water samples. The results suggest that surface water harbors various virulent V. cholerae strains that contaminate the ground water due to rain or poor hygienic practices, and result in the emergence of new toxigenic strains for cholera.
- MeSH
- bakteriální proteiny genetika MeSH
- cholera epidemiologie mikrobiologie MeSH
- DNA fingerprinting MeSH
- DNA vazebné proteiny genetika MeSH
- endemické nemoci MeSH
- faktory virulence genetika MeSH
- genotyp MeSH
- hemolyziny genetika MeSH
- lidé MeSH
- mikrobiologie vody MeSH
- proteiny vnější bakteriální membrány genetika MeSH
- roční období MeSH
- shluková analýza MeSH
- techniky typizace bakterií MeSH
- transkripční faktory genetika MeSH
- Vibrio cholerae izolace a purifikace klasifikace patogenita MeSH
- virulence MeSH
- Check Tag
- lidé MeSH
- Geografické názvy
- Indie MeSH
- MeSH
- cholera diagnóza MeSH
- epidemiologické studie MeSH
- lidé MeSH
- Vibrio cholerae izolace a purifikace MeSH
- Check Tag
- lidé MeSH
- Geografické názvy
- Slovenská republika MeSH
- MeSH
- cholera epidemiologie mortalita MeSH
- hlášení nemocí MeSH
- Světová zdravotnická organizace MeSH
- Vibrio cholerae izolace a purifikace MeSH
- Geografické názvy
- Afrika MeSH
- Asie MeSH
- MeSH
- cestování MeSH
- cholera diagnóza etiologie terapie MeSH
- dospělí MeSH
- lidé MeSH
- terapie metody MeSH
- Vibrio cholerae fyziologie izolace a purifikace klasifikace MeSH
- všeobecná preventivní opatření MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- Geografické názvy
- Indie MeSH
