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19 záznamů v Medvik Filtry

Článek

Transgenic mouse model expressing tdTomato under involucrin promoter as a tool for analysis of epidermal differentiation and wound healing

Transgenic research. 2012 ; 21 (3) : 683-9.

Transgenic Res
ISSN 1573-9368
Medvik
Zdroj

The epidermis is a stratified tissue composed of different keratinocyte layers that create a barrier protecting the body from external influences, pathogens, and dehydration. The barrier function is mainly achieved by its outermost layer, the stratum corneum. To create a mouse model to study pathophysiological processes in the outermost layers of the epidermis in vivo and in vitro we prepared a construct containing red fluorescent td-Tomato reporter sequence under the control of involucrin promoter and its first intron. Transgenic mice were generated by pronuclear injection and the expression and regulation of the transgene was determined by in vivo imaging and fluorescent microscopy. The promoter targeted the transgene efficiently and specifically into the outermost epidermal layers although weak expression was also found in epithelia of tongue and bladder. The regulation of expression in the epidermis, i.e. fluorescence intensity of the reporter, could be easily followed during wound healing and dermatitis. Thus, these transgenic mice carrying the tdTomato reporter could be used as a valuable tool to study impact of various genes dysregulating the epidermal barrier and to follow effects of therapeutic agents for treatment of skin diseases in vivo.

MeSH
buněčná diferenciace MeSH
dermatitida metabolismus patofyziologie MeSH
epidermis metabolismus patofyziologie MeSH
epitel metabolismus patofyziologie MeSH
fluorescenční mikroskopie MeSH
genový targeting metody MeSH
hojení ran MeSH
imunohistochemie MeSH
introny MeSH
jazyk metabolismus patofyziologie MeSH
luminescentní proteiny metabolismus MeSH
myši inbrední C57BL MeSH
myši transgenní MeSH
myši MeSH
promotorové oblasti (genetika) MeSH
proteinové prekurzory genetika MeSH
regulace genové exprese MeSH
reportérové geny MeSH
Solanum lycopersicum genetika MeSH
transgeny MeSH
zvířata MeSH
Check Tag
myši MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH

Článek

The role of autophagy in the midgut epithelium of Eubranchipus grubii (Crustacea, Branchiopoda, Anostraca)

Arthropod structure and development. 2012 ; 41 (3) : 271-279. [pub] 20120322

Arthropod Struct Dev
ISSN 1873-5495
Medvik
Zdroj

Eubranchipus grubii (Crustacea, Branchiopoda, Anostraca) is an omnivorous filter feeder whose life span lasts no more than 12 weeks. Adult males and females of E. grubii were used for ultrastructural studies of the midgut epithelium and an analysis of autophagy. The midgut epithelium is formed by columnar digestive cells and no regenerative cells were observed. A distinct regionalization in the distribution of organelles appears - basal, perinuclear and apical regions were distinguished. No differences in the ultrastructure of digestive cells were observed between males and females. Autophagic disintegration of organelles occurs throughout the midgut epithelium. Degenerated organelles accumulate in the neighborhood of Golgi complexes, and these complexes presumably take part in phagophore and autophagosome formation. In some cases, the phagophore also surrounds small autophagosomes, which had appeared earlier. Fusion of autophagosomes and lysosomes was not observed, but lysosomes are enclosed during autophagosome formation. Autophagosomes and autolysosomes are discharged into the midgut lumen due to apocrine secretion. Autophagy plays a role in cell survival by protecting the cell from cell death.