Astroviruses are a major cause of gastroenteritis in humans and animals. Recently, novel groups of astroviruses were identified in apparently healthy insectivorous bats. We report the detection of diverse novel astrovirus sequences in nine different European bat species: Eptesicus serotinus, Hypsugo savii, Myotis emarginatus, M. mystacinus, Nyctalus noctula, Pipistrellus nathusii or P. pygmaeus, P. pipistrellus, Vespertilio murinus, and Rhinolophus hipposideros. In six bat species, astrovirus sequences were detected for the first time. One astrovirus strain detected in R. hipposideros clustered phylogenetically with Chinese astrovirus strains originating from bats of the families Rhinolophidae and Hipposideridae. All other Czech astrovirus sequences from vesper bats formed, together with one Hungarian sequence, a separate monophyletic lineage within the bat astrovirus group. These findings provide new insights into the molecular epidemiology, ecology, and prevalence of astroviruses in European bat populations.
- MeSH
- Astroviridae genetika izolace a purifikace MeSH
- Chiroptera virologie MeSH
- fylogeneze MeSH
- gastroenteritida epidemiologie veterinární virologie MeSH
- genetická variace * MeSH
- genom virový genetika MeSH
- infekce viry z čeledi Astroviridae epidemiologie veterinární virologie MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- sekvence nukleotidů MeSH
- sekvenční analýza DNA MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
Small, non-enveloped RNA viruses belonging to the genera Sapovirus, Kobuvirus, and Mamastrovirus are usually associated with gastroenteritis in humans and animals. These enteric pathogens are considered potential zoonotic agents. In this study, the prevalence and genetic diversity of sapoviruses (SaVs), kobuviruses (KoVs), and astroviruses (AstVs) in asymptomatic pigs were investigated using a PCR approach. KoV was found to be the most prevalent virus (87.3 %), followed by AstV (34.2 %) and SaV (10.2 %). Interestingly, the intra- and inter-cluster distances between porcine SaV capsid sequences revealed one strain (P38/11/CZ) that formed a new genotype within genogroup III of porcine SaVs, and it is tentatively called "P38/11-like" genotype. Moreover, this is the first report of porcine kobuvirus detection on Czech pig farms. The high prevalence rate of gastroenteritis-producing viruses in clinically healthy pigs represents a continuous source of infection of pigs, and possibly to humans.
- MeSH
- Astroviridae genetika MeSH
- fylogeneze MeSH
- gastroenteritida epidemiologie veterinární virologie MeSH
- genetická variace * MeSH
- genotyp MeSH
- infekce viry z čeledi Astroviridae epidemiologie veterinární virologie MeSH
- infekce viry z čeledi Caliciviridae epidemiologie veterinární virologie MeSH
- Kobuvirus genetika MeSH
- nemoci prasat epidemiologie virologie MeSH
- pikornavirové infekce epidemiologie veterinární virologie MeSH
- prasata MeSH
- RNA virová genetika MeSH
- Sapovirus klasifikace genetika MeSH
- sekvenční analýza RNA MeSH
- Sus scrofa MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
- MeSH
- gastroenteritida epidemiologie veterinární MeSH
- nemoci skotu epidemiologie MeSH
- skot MeSH
- virové nemoci epidemiologie veterinární MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Geografické názvy
- Československo MeSH
