The effect of three-day fasting on cardiac ischemic tolerance was investigated in adult male Wistar rats. Anesthetized open-chest animals (pentobarbitone 60 mg/kg, i.p.) were subjected to 20-min left anterior descending coronary artery occlusion and 3-h reperfusion for infarct size determination. Ventricular arrhythmias were monitored during ischemia and at the beginning (3 min) of reperfusion. Myocardial concentrations of beta-hydroxybutyrate and acetoacetate were measured to assess mitochondrial redox state. Short-term fasting limited the infarct size (48.5+/-3.3 % of the area at risk) compared to controls (74.3+/-2.2 %) and reduced the total number of premature ventricular complexes (12.5+/-5.8) compared to controls (194.9+/-21.9) as well as the duration of ventricular tachycardia (0.6+/-0.4 s vs. 18.8+/-2.5 s) occurring at early reperfusion. Additionally, fasting increased the concentration of beta-hydroxybutyrate and beta-hydroxybutyrate/acetoacetate ratio (87.8+/-27.0) compared to controls (7.9+/-1.7), reflecting altered mitochondrial redox state. It is concluded that three-day fasting effectively protected rat hearts against major endpoints of acute I/R injury. Further studies are needed to find out whether these beneficial effects can be linked to altered mitochondrial redox state resulting from increased ketogenesis.
- MeSH
- acetoacetáty metabolismus farmakologie MeSH
- infarkt myokardu metabolismus veterinární MeSH
- komorová tachykardie komplikace MeSH
- krysa rodu rattus MeSH
- kyselina 3-hydroxymáselná metabolismus farmakologie MeSH
- mitochondrie metabolismus MeSH
- oxidace-redukce MeSH
- potkani Wistar MeSH
- reperfuzní poškození myokardu metabolismus veterinární MeSH
- srdeční arytmie patofyziologie veterinární MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- finanční podpora výzkumu jako téma MeSH
- hematopoetické kmenové buňky cytologie MeSH
- infarkt myokardu patofyziologie veterinární MeSH
- králíci anatomie a histologie MeSH
- modely nemocí na zvířatech MeSH
- železo diagnostické užití MeSH
- zvířata MeSH
- Check Tag
- králíci anatomie a histologie MeSH
- zvířata MeSH
