- MeSH
- diabetes mellitus 1. typu farmakoterapie MeSH
- hypoglykemie prevence a kontrola MeSH
- inzulin aplikace a dávkování normy MeSH
- inzulinové infuzní systémy normy trendy MeSH
- lidé MeSH
- selfmonitoring glykemie * metody přístrojové vybavení MeSH
- senzitivita a specificita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- multicentrická studie MeSH
- randomizované kontrolované studie MeSH
Léčba inzulinovou pumpou je dnes už běžnou možností podávání inzulínu pro pacienty s diabetes mellitus. Převážně jsou takto léčeni pacienti s diabetem 1. typu. Práce shrnuje praktický postup při indikaci a zahájení léčby. Podává přehled dostupných přístrojů. Současně ukazuje i možnosti využití kontinuální monitorace při léčbě pumpou.
Insulin pump treatment is now a common option for insulin delivery for patients with diabetes mellitus. Patients with type 1 diabetes are predominantly treated. The paper summarizes the practical procedure for indicating and initiating treatment. Provides an over view of available devices. At the same time, its hows the possibilities of using continuous monitoring during pump treatment.
- MeSH
- diabetes mellitus farmakoterapie MeSH
- inzulin aplikace a dávkování normy MeSH
- inzulinové infuzní systémy * normy škodlivé účinky MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- práce podpořená grantem MeSH
Criteria for the evaluation of the insulin tolerance test (ITT) and Synacthen test are still a matter of debate. The objective of the study was to make a comparison of serum and salivary cortisol during four stimulation tests. Sixty four healthy volunteers underwent the ITT, the Synacthen test with 1 (LDST), 10 (MDST) and 250 (HDST) microg dose of ACTH. Maximum serum cortisol response was observed at the 90 min of the ITT (49 %), HDST (89 %) and MDST (56 %) and at the 40 min of the LDST (44 %). Results expressed as 95 % confidence intervals: 408.0-843.6 and 289.5-868.1 nmol/l in the IIT at 60 and 90 min. In the HDST and the MDST serum cortisol reached the maximum at 90 min 542.6-1245.5 and 444.2-871.3 nmol/l. Levels of salivary cortisol followed the same pattern as serum cortisol. Salivary cortisol reached the maximum response in the HDST and the MDST at 90 min and at 40 min in the LDST. We confirmed good reliability of all tests with respect to timing of response and maximum response compared to the ITT. We proved that the MDST test can provide the similar response in serum cortisol to the HDST. Measuring either salivary cortisol or ACTH levels did not provide any additional benefit then measuring serum cortisol by itself.
- MeSH
- dospělí MeSH
- hydrokortison analýza krev MeSH
- inzulin aplikace a dávkování normy MeSH
- inzulinová rezistence fyziologie MeSH
- kosyntropin aplikace a dávkování normy MeSH
- lidé MeSH
- referenční standardy MeSH
- reprodukovatelnost výsledků MeSH
- sliny chemie metabolismus MeSH
- systém hypofýza - nadledviny účinky léků metabolismus MeSH
- systém hypotalamus-hypofýza účinky léků metabolismus MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- pozorovací studie MeSH
- srovnávací studie MeSH
The type of the stationary phase for reversed-phase liquid chromatography significantly affects the sample elution. Hydrodynamic properties, efficiency and gradient elution of proteins were investigated on five commercial C18 columns with wide-pore totally porous particles, with superficially porous layer particles, non-porous particles and a silica-based monolithic bed. The efficiency in the terms of reduced plate height is higher for low-molecular ethylbenzene than for proteins, but depends on the character of the pores in the individual columns tested. The superficially porous Poroshell and the non-porous Micra columns provide the best efficiency for proteins at high mobile phase flow rates, probably because of similar pore architecture in the stationary phase. The Zorbax column with similar pore architecture as the Poroshell active layer, i.e. narrow pore distribution of wider pores shows better efficiency than the packed column with narrow pores and broad pore distribution. The monolithic column shows lower efficiency for proteins at high flow rates, but it performs better than the broad-pore distribution totally porous particulate columns. Different pore architecture affects also the retention and selectivity for proteins on the individual columns. The retention times on all columns can be predicted using the model for reversed-phase gradient elution developed originally for low-molecular compounds. Consideration of the limited pore volume accessible to the biopolymers has negligible effect on the prediction of retention on the columns packed with non-porous or superficially porous particles, but improves the accuracy of the predicted data for the totally porous columns with broad pore distribution.
- MeSH
- algoritmy MeSH
- chemické modely MeSH
- financování organizované MeSH
- hydrofobní a hydrofilní interakce MeSH
- inzulin normy MeSH
- lidé MeSH
- peptidy analýza chemie izolace a purifikace MeSH
- poréznost MeSH
- proteiny analýza chemie izolace a purifikace MeSH
- reprodukovatelnost výsledků MeSH
- sérový albumin normy MeSH
- trypsin normy MeSH
- velikost částic MeSH
- vysokoúčinná kapalinová chromatografie metody přístrojové vybavení MeSH
- Check Tag
- lidé MeSH
- MeSH
- inzulin farmakologie normy zásobování a distribuce MeSH
- lékové formy MeSH
- Geografické názvy
- Česká republika MeSH
