Ischemic stroke is a well-recognized disease of aging, yet it is unclear how the age-dependent vulnerability occurs and what are the underlying mechanisms. To address these issues, we perform a comprehensive RNA-seq analysis of aging, ischemic stroke, and their interaction in 3- and 18-month-old mice. We assess differential gene expression across injury status and age, estimate cell type proportion changes, assay the results against a range of transcriptional signatures from the literature, and perform unsupervised co-expression analysis, identifying modules of genes with varying response to injury. We uncover downregulation of axonal and synaptic maintenance genetic program, and increased activation of type I interferon (IFN-I) signaling following stroke in aged mice. Together, these results paint a picture of ischemic stroke as a complex age-related disease and provide insights into interaction of aging and stroke on cellular and molecular level.
- MeSH
- ischemická cévní mozková příhoda metabolismus MeSH
- ischemie mozku genetika metabolismus MeSH
- modely nemocí na zvířatech MeSH
- mozek metabolismus MeSH
- myši MeSH
- reperfuzní poškození metabolismus MeSH
- stárnutí fyziologie MeSH
- zvířata MeSH
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- myši MeSH
- zvířata MeSH
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- časopisecké články MeSH
- práce podpořená grantem MeSH
