The Francisella tularensis strain LVS phagosome disintegrates during the first few hours after bacterial entry and microbes are released to the cytosol. Within 12 h both rapid multiplication of microbes and a steep increase of apoptosis of infected macrophages occur. We searched for signals involved in the death of macrophages and detected molecules associated with the autophagy machinery cathepsin D, PTEN, p53 and LC3, whose levels or modification were influenced by ongoing in vitro tularemic infection. The sequestration of cytoplasmic F. tularensis LVS into autophagosomes was confirmed by co-localization of the LVS strain containing vacuoles with LC3 (an autophagosomal marker). We also demonstrated the presence of MHC II antigens in these autophagosomes, indicating that they might act as a source of endogenous tularemic antigens for presentation to CD4+ T lymphocytes.

• MeSH
• buněčná smrt genetika   imunologie   MeSH
• cytosol fyziologie   imunologie   mikrobiologie   MeSH
• financování organizované využití   MeSH
• fluorescenční mikroskopie využití   MeSH
• fosfohydroláza PTEN genetika   imunologie   izolace a purifikace   MeSH
• Francisella tularensis imunologie   izolace a purifikace   MeSH
• imunoblotting metody   využití   MeSH
• kathepsin D genetika   imunologie   izolace a purifikace   MeSH
• makrofágy imunologie   mikrobiologie   MeSH
• tularemie etiologie   imunologie   mikrobiologie   MeSH

• MeSH
• anafylaxe MeSH
• kathepsin D imunologie   MeSH
• modely u zvířat MeSH
• morčata MeSH
• prezentace antigenu MeSH
• Check Tag
• morčata MeSH