Fifty-five strains of enterococci isolated from the piglet intestine were characterized in vitro for probiotic activity. Identification of the isolates revealed Enterococcus faecium as the predominant species (84 %). Forty strains (73 %) were found to produce bacteriocin-like substances (only into solid media) with activity almost only toward Gram-positive genera. Thirty-eight % of strains were resistant to tetracycline, 27 % to chloramphenicol, 18 % to erythromycin and 16 % to vancomycin. In addition to control of strain safety, 6 % of isolates were beta-hemolytic and 16 % produced gelatinase. Seven strains selected for further probiotic assays exhibited sufficient survival rate at pH 3.0 after 3 h, in the presence of 1 % ox-bile and lysozyme after 1 d (over 107 CFU/mL in all tests). The adhesion of tested strains to porcine and human intestinal mucus was found in a similar range (1.4-14.0 % and 1.4-17.6 %, respectively). In accordance with current research effort to use and/or to combine various health promoting substances, the sensitivity of all isolates toward plant extracts and toward bacteriocins produced by animal and environmental strains was determined. All enterococci were sensitive toward oregano and sage extracts and toward one (E. faecium EF55--chicken isolate, activity of 25 600 AU/mL) of ten bacteriocin substances. It means that a similar anti-enterococcal potential of some bacteriocin substances may be observed as for certain plant extracts.
- MeSH
- antibakteriální látky farmakologie MeSH
- antibióza MeSH
- bakteriální adheze MeSH
- bakteriální léková rezistence MeSH
- bakteriociny biosyntéza farmakologie MeSH
- dobromysl (rod) chemie MeSH
- Enterococcus MeSH
- financování organizované MeSH
- gastrointestinální trakt mikrobiologie MeSH
- kyseliny toxicita MeSH
- mikrobiální testy citlivosti MeSH
- mikrobiální viabilita MeSH
- muciny metabolismus MeSH
- muramidasa MeSH
- prasata mikrobiologie MeSH
- probiotika farmakologie MeSH
- rostlinné extrakty farmakologie MeSH
- žlučové kyseliny a soli toxicita MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
Corrosive injuries of the upper gastrointestinal tract in children are among the most serious and relatively frequent conditions with complicated treatment and long-term or permanent consequences. We observed a set of patients at our department (hereinafter referred to as KDCHOT FN Brno) between 01/01/2003 and 31/12/2007. During the period of 5 years, we treated 120 children with various degrees of corrosive injury of the upper digestive tract (oral cavity, pharynx, oesophagus, and stomach) or with suspected injuries of this type. In more than one third of the patients out of a total of 120, following ingestion of an allochthonous substance no signs of damage to the skin or the mucous membrane were found on ear, nose and throat examination. Fifty-nine patients exhibited 1st-degree burns according to the classification below, 15 patients exhibited 2nd-degree burns of the oesophagus or the stomach, and only in 2 patients we observed 3rd-degree burns of the oesophagus with complicated treatment. Complete healing occurred in all patients; patients with 3rd-degree burns achieved complete healing after a series of oesophageal dilatations. With regard to our experience showing that minimal damage to the oral cavity, pharynx, and proximal oesophagus is frequently associated with significantly more serious burns of the distal oesophagus and stomach, we regard oesophagogastroscopy and verification of the condition of oesophageal and gastric mucosa as necessary in terms of diagnostic standards for this type of injury in children.
- MeSH
- antacida terapeutické užití MeSH
- antibakteriální látky terapeutické užití MeSH
- chemické popálení etiologie komplikace terapie MeSH
- detergenty škodlivé účinky toxicita MeSH
- dezinficiencia škodlivé účinky toxicita MeSH
- endoskopie trávicího systému využití MeSH
- ezofagitida diagnóza etiologie komplikace MeSH
- ezofágus patologie zranění MeSH
- farmakoterapie metody využití MeSH
- hormony kůry nadledvin terapeutické užití MeSH
- horní gastrointestinální trakt patologie zranění MeSH
- kyseliny škodlivé účinky toxicita MeSH
- lidé MeSH
- louh škodlivé účinky toxicita MeSH
- předškolní dítě MeSH
- žaludek patologie zranění MeSH
- Check Tag
- lidé MeSH
- předškolní dítě MeSH
