- MeSH
- adenosindifosfát biosyntéza fyziologie metabolismus MeSH
- ATP-synthetasa (komplexy) biosyntéza fyziologie metabolismus MeSH
- energetický metabolismus MeSH
- kosterní svalová vlákna fyziologie metabolismus MeSH
- kosterní svaly * cytologie fyziologie metabolismus MeSH
- kreatinkinasa, mitochondriální forma MeSH
- kreatinkinasa * biosyntéza fyziologie metabolismus MeSH
- lidé MeSH
- myofibrily fyziologie metabolismus MeSH
- myosiny MeSH
- svalové mitochondrie fyziologie metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Endotoxin administration is frequently used as a model of systemic inflammatory response which is considered the important pathogenetic factor in muscle wasting development in severe illness, such as sepsis, cancer, injury, AIDS and others. The main purpose of this study was determining the effect of various doses of endotoxin on protein and amino acid metabolism in two types of rat skeletal muscle. Sepsis was induced by intraperitoneal administration of endotoxin in a dose of 1, 3 and 5 mg/kg body weight (bw); control animals received a corresponding volume of the saline solution. After 24 h, extensor digitorum longus (EDL) and soleus (SOL) muscles were isolated and used for determination of total and myofibrillar proteolysis, protein synthesis, activity of cathepsins B and L, chymotrypsin-like activity of proteasome and amino acid release. The endotoxemia induced the body weight loss, the rise of total cholesterol and triglyceride plasma concentration and the protein catabolic state in skeletal muscle, which was caused by a higher increase in protein breakdown (due to activation of the proteasome system) than protein synthesis. The more significant effect of endotoxin was seen in EDL than SOL. The dose of 5 mg of endotoxin/kg bw induced the most significant changes in parameters of the protein and amino acid metabolism measured and could be therefore considered appropriate for studies of protein catabolism in young rat skeletal muscle at 24 h after endotoxin treatment.
- MeSH
- endotoxiny farmakologie MeSH
- kathepsin B metabolismus MeSH
- kosterní svaly metabolismus MeSH
- krysa rodu rattus MeSH
- myofibrily metabolismus MeSH
- potkani Wistar MeSH
- proteasomový endopeptidasový komplex metabolismus MeSH
- sepse metabolismus MeSH
- svalové proteiny metabolismus MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
We examined the effects of the unilateral heterochronous isotransplantation on the fiber type composition and myosin heavy chain (MyHC) isoform content of unoperated slow soleus and fast extensor digitorum longus muscles of female inbred Lewis strain rats. Comparison was made between "control" unoperated muscles of experimental rats (after intramuscular transplantation surgery) with the corresponding muscles of completely naive (unoperated) rats of three age groups (5-, 8- and 14-month-old). This was done in order to ascertain whether these muscles can be used as reliable controls to the transplanted and host muscles for our ongoing grafting experiments. The fiber type composition was determined by assessing the histochemical reaction for myofibrillar adenosine triphosphatase, the MyHC isoform content was determined immunocytochemically using monoclonal antibodies specific to different MyHC isoforms and by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Our experiments show that the heterochronous intramuscular isotransplantation procedure had no significant effect on the fiber type composition and MyHC isoform content of the "control" unoperated muscles of the experimental rats when compared to the corresponding muscles of the naive animals. Furthermore, the duration and type of isotransplantation did not also lead to differences among corresponding "control" muscles of experimental animals. We conclude that the unoperated muscles of the experimental rats can be used as controls in our current transplantation project dealing with long-term grafting experiments.
- MeSH
- adenosintrifosfatasy metabolismus MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- financování organizované MeSH
- homologní transplantace MeSH
- imunohistochemie MeSH
- isomerie MeSH
- kosterní svaly cytologie transplantace MeSH
- krysa rodu rattus MeSH
- myofibrily metabolismus MeSH
- potkani inbrední LEW MeSH
- přežívání štěpu MeSH
- svalová vlákna typu I cytologie metabolismus MeSH
- svalová vlákna typu II cytologie metabolismus MeSH
- těžké řetězce myosinu chemie metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- MeSH
- chronické selhání ledvin patologie terapie MeSH
- lidé MeSH
- myofibrily metabolismus MeSH
- nemoci srdce metabolismus terapie MeSH
- prediktivní hodnota testů MeSH
- troponin I analýza krev MeSH
- troponin T analýza krev MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
- MeSH
- myofibrily fyziologie metabolismus MeSH
- svalová kontrakce fyziologie MeSH
- vápníkové kanály MeSH
- Publikační typ
- přehledy MeSH
