Identification of genes that are important for normal preimplantation development is essential for understanding the basics of early mammalian embryogenesis. In our previous study, we have shown that CENPF (mitosin) is differentially expressed during preimplantation development of bovine embryos. CENPF is a centromere-kinetochore complex protein that plays a crucial role in the cell division of somatic cells. To our best knowledge, no study has yet been done on either bovine model, or oocytes and preimplantation embryos. In this study, we focused on the fate of bovine embryos after injection of CENPF double-stranded RNA (dsRNA) into the zygotes. An average decrease of CENPF mRNA abundance by 94.9% or more and an extensive decline in immunofluorescence staining intensity was detected relative to controls. There was no disparity between individual groups in the developmental competence before the 8-cell stage. However, the developmental competence rapidly decreased then and only 28.1% of CENPF dsRNA injected 8-cell embryos were able to develop further (uninjected control: 71.8%; green fluorescent protein dsRNA injected control: 72.0%). In conclusion, these results show that depletion of CENPF mRNA in preimplantation bovine embryos leads to dramatic decrease of developmental competence after embryonic genome activation.
- MeSH
- blastocysta cytologie metabolismus fyziologie MeSH
- buněčné dělení genetika fyziologie MeSH
- chromozomální proteiny, nehistonové antagonisté a inhibitory genetika metabolismus MeSH
- embryonální vývoj genetika fyziologie MeSH
- fertilizace in vitro veterinární MeSH
- kontrolní body buněčného cyklu genetika fyziologie MeSH
- kultivované buňky MeSH
- mikrofilamentové proteiny antagonisté a inhibitory genetika metabolismus MeSH
- skot embryologie genetika fyziologie MeSH
- stadium rýhování vajíčka metabolismus fyziologie MeSH
- umlčování genů fyziologie MeSH
- vývojová regulace genové exprese MeSH
- zvířata MeSH
- Check Tag
- skot embryologie genetika fyziologie MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
