Meconium aspiration syndrome (MAS) is meconium-induced respiratory failure of newborns associated with activation of inflammatory and oxidative pathways. For severe MAS, exogenous surfactant treatment is used which improves respiratory functions but does not treat the inflammation. Oxidative process can lead to later surfactant inactivation; hence, surfactant combination with antioxidative agent may enhance the therapeutic effect. Young New Zealand rabbits were instilled by meconium suspension and treated by surfactant alone, N-acetylcysteine (NAC) alone or by their combination and oxygen-ventilated for 5 h. Blood samples were taken before and 30 min after meconium application and 30 min, 1, 3 and 5 h after the treatment for evaluating of oxidative damage, total leukocyte count, leukocyte differential count and respiratory parameters. Leukocyte differential was assessed also in bronchoalveolar lavage fluid. NAC alone had only mild therapeutic effect on MAS. However, the combination of NAC and surfactant facilitated rapid onset of therapeutic effect in respiratory parameters (oxygenation index, PaO(2)/FiO(2)) compared to surfactant alone and was the only treatment which prevented neutrophil migration into the lungs, oxidative damage and lung edema. Moreover, NAC suppressed IL-8 and IL-beta formation and thus seems to be favorable agent for improving surfactant therapy in MAS.
- MeSH
- acetylcystein farmakologie terapeutické užití MeSH
- bronchoalveolární lavážní tekutina imunologie MeSH
- cytokiny metabolismus MeSH
- edém prevence a kontrola MeSH
- expektorancia farmakologie terapeutické užití MeSH
- kombinovaná farmakoterapie MeSH
- králíci MeSH
- látky reagující s kyselinou thiobarbiturovou metabolismus MeSH
- náhodné rozdělení MeSH
- novorozená zvířata MeSH
- pilotní projekty MeSH
- plíce účinky léků metabolismus MeSH
- plicní surfaktanty farmakologie terapeutické užití MeSH
- počet leukocytů MeSH
- preklinické hodnocení léčiv MeSH
- respirační funkční testy MeSH
- syndrom aspirace mekonia imunologie metabolismus prevence a kontrola MeSH
- testy migrace leukocytů MeSH
- tyrosin analogy a deriváty metabolismus MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
